Spectrophotometric Determination of Naproxen in Pure and Pharmaceutical Preparations (original) (raw)
Related papers
Current Chemistry Letters, 2013
A highly sensitive, accurate and simple spectrophotometric method was established for determination of naproxen (NAP). The method involved ion-pair complex formation between naproxen and bromophenol blue (BPB). The colored product was measured at 432 nm. The reaction conditions were optimized. The absorbance was found to increase linearly with increase in concentration of NAP which was corroborated by correlation coefficient value. Beer's law was obeyed in the concentration range of 1-110 µg mL -1 with molar absorptivity of 9.756×10 3 L mol -1 cm -1 . The limits of detection (LOD) and quantitation (LOQ) for the proposed method are 0.292 and 0.973 µg mL -1 , respectively. Recovery of the method was carried out by standard addition method. Recovery studies and statistical data proved the accuracy, reproducibility and the precision of the proposed method. The common excipients did not interfere in this analysis. Hence the method is useful for routine estimation of naproxen in pharmaceutical formulation and human serum samples.
Spectrofluorometric determination of naproxen in tablets
Journal of Pharmaceutical and Biomedical Analysis, 2002
A rapid, selective, sensitive and simple fluorescence method was developed for the direct determination of naproxen in tablets. The tablets were triturated, dissolved in either NH 3 or NaOH solution, sonicated, filtered and then direct fluorescence emission was read at 353 nm (exciting at 271 nm). In order to validate the method the results were compared with those obtained by the USP XXIV NF 19 Pharmacopeia reference method (high performance liquid chromatography). The slope, intercept and variances which are associated with the regression coefficient calculated with bivariate least square (BLS) regression indicate that both methods are statistically comparable. The recoveries were excellent, except in tablets containing the antibiotic tetracycline. In this latter case a correction procedure is necessary.
A concise review on analytical profile of naproxen
IP innovative publication pvt. ltd, 2019
Naproxen (NAP) is a Non-steroidal anti-inflammatory drugs (NSAID) used in the treatment of pain or inflammation caused by situations such as arthritis, ankylosing spondylitis, tendinitis, bursitis, gout, or menstrual cramps. Nap is available in isolated dosage from with various similar anti-inflammatory drugs, esomeprazole, pantoprazole, paracetamol, ranitidine, sumatriptan and ibuprofen. The present exploration evaluates the various method for analysing of NAP in bulk drugs and formulated products. A summarizing review characterizes the gathering and conversation of about more than 62 analytical methods which includes HPLC, HPTLC, UV-Spectrophotometry, capillary electrophoresis, electrochemical methods. HPLC technique are provided in Table03 and Table 04 for NAP alone and combination, including parameters such as matrix, stationary phase, mobile phase, wavelength detection etc. and HPTLC methods are reported in Table 05 with parameters like stationary phase, mobile phase combination, R F etc. Method of UV-Spectrophotometry applied for examination of NAP in biological mediums, bulk sample and in various dosage formulation. Spectrometric methods for NAP alone and in mixture are given in Table 08 which includes parameters like λ max, solvent, matrix etc.
Bangladesh Pharmaceutical Journal, 2015
A simple, sensitive and precise reversed phase high performance liquid chromatographic (RP-HPLC) method has been developed for the estimation of naproxen in pharmaceutical dosage forms. The method was developed using the mobile phase comprising of dibasic sodium phosphate buffer (Na 2 HPO 4 ) at pH 7.80 (adjusted by sodium hydroxide) and acetonitrile in the ratio of 70:30 (v/v) over C-18 column (250 x 4.6 mm, 5µm, Phenomenex Inc.) at ambient temperature. The flow rate was at 0.7 ml/min and the column washing was monitored by UV detector at 225 nm. The retention time of naproxen was 4.8 ± 0.1 min. The recovery was found to be >97% which is demonstrative of accuracy of the protocol. Inter-day and intra-day precision of the newly developed method were less than the maximum allowable limit (RSD% ≤ 2.0) according to ICH, USP and FDA guidelines. The method showed linear response with correlation coefficient (r 2 ) value of 0.9991. Therefore, the method was found to be accurate, reproducible, sensitive and less time consuming and can be successfully applied for routine analysis of naproxen in pharmaceutical formulations.
1999
A simple, selective and sensitive heavy atom-induced room temperature phosphorimetric method (HAI-RTP) is described for the determination of naproxen (NAP) in pharmaceutical preparations. The phosphorescence signals are a consequence of intermolecular protection when analytes are, exclusively, in presence of a heavy atom salt and sodium sulfite as an oxygen scavenger to minimize RTP quenching. These variables selection constitute the basis of a HAI-RTP method for the determination of naproxen (detection limit 17.6 ng ml − 1 ; 1.71% relative standard deviation at 250 ng ml − 1). The method has been applied satisfactorily to the analysis of pharmaceutical preparations.
Bioanalytical Validated LC-MS Method for Determination of Naproxen in Human Plasma
Naproxen is an anti-inflammatory drug belongs to the category of analgesics and antipyretics. Naproxen has the ability to bind and inhibit the synthesis of prostaglandins and produces anti-inflammatory effect. Naproxen also inhibits COX-II which is involved in the inflammation. Bioanalytical method for naproxen has been developed using human plasma. Standard stock solution of naproxen was prepared using methanol. Zidovudine was used as internal standard. The linearity of the proposed method was developed in the range between 100 and 10000 ng/ml. The slope of the curve was found to be 111.46 and intercept was 6395.2 and correlation coefficient was 0.999. The proposed method was evaluated with validation parameters like accuracy, precision and stability. The developed method is suitable for pharmacokinetic studies.
Voltammetric and Chromatographic Determination of Naproxen in Drug Formulation
Journal of Scientific Perspectives, 2019
In this work, the electrochemical oxidation of naproxen (NAP) was studied at an ultra-trace graphite electrode (UTGE). The cyclic voltammetry (CV) technique was used to determine the optimum conditions and the effect of pH on the electrochemical oxidation of NAP. Acetate buffer (pH 4.50) was selected as the support electrolyte due to obtaining the highest electronic signal increase during oxidation of NAP at UTGE. The differential pulse voltammetry (DPV) technique was performed for electrochemical determination of NAP. In the optimum conditions, the limits of detection (LOD) and quantification (LOQ) were determined to be 8.66 10-8 M and 2.88 10-7 M. In addition, the amount of NAP was determined in drug tablets. The recovery studies of NAP from the drug tablet were completed in order to check the accuracy and precision of the applied voltammetric method. Furthermore, the determination of NAP was performed with the high-performance liquid chromatography (HPLC) method. These two methods were compared in terms of accuracy, precision and recovery studies.
2014
Purpose: To determine naproxen levels in human plasma using a new liquid chromatography-Mass spectroscopy/Mass spectroscopy (LC-MS/MS) method that involves a simple and single step extraction procedure using low-cost reagents. Method: A novel liquid chromatography‐tandem mass spectrometry method for the quantitative determination of naproxen in human K2-EDTA plasma in negative ion mode was employed and validated using zidovudine as internal standard (IS). Sample preparation was accomplished by liquidliquid extraction technique. The eluted samples were chromatographed on Zorbax Eclipse XDB phenyl 4.6 ◊ 75 mm, 3.5 µm column (Agilent Technologies) using a mobile phase consisting of acetonitrile: 20 mM ammonium acetate (90:10 v/v).The injection volume was 15 µL and the total run time was 3.0 min. The method was validated for all parameters for naproxen. Results: The method showed selectivity and linearity over a concentration range of 500.1 ng/mL to 100028.5 ng/mL The validation data in...
Stereoselective determination of S-naproxen in tablets by capillary electrophoresis
Journal of Pharmaceutical and Biomedical Analysis, 1998
A capillary electrophoresis (CE) method was developed for the stereoselective determination of the non-steroidal anti-inflammatory drug (NSAID), S-naproxen, in tablets. Several i-cyclodextrin derivatives (CDs) were tested as chiral selectors, including sulfobutyl-i-CD (SBCD), carboxymethyl-i-CD (CMCD), dimethyl-i-CD (DMCD) and trimethyl-i-CD (TMCD), in a phosphoric acid/triethanolamine pH 3 buffer. Under these conditions, the analyte was mainly present in an uncharged form and therefore, the use a neutral CD (DMCD or TMCD) alone could not lead to enantiomeric separation. On the contrary, by addition of a charged CD (SBCD or CMCD) to the running buffer, giving the analyte enantiomers an adequate mobility, chiral resolution could be achieved, although the resolution values obtained in this case were not quite satisfactory (RsB 1.5). Dual systems, based on the use of mixtures of charged and neutral CDs, were then investigated. The SBCD/TMCD system was found to be particularly well suited to the enantioseparation of naproxen and after optimisation of the concentrations of both CDs, a resolution value of 5.4 could be obtained. The method was validated for the determination of R-naproxen (enantiomeric impurity) in the 0.1-2% range, using the racemic mixture of the analyte. A second validation was performed in the 50 -150% range for the quantitation of S-naproxen. In both cases, good results with respect to linearity, precision and accuracy were obtained.