Anticancer Activity of Papaver Somniferum (original) (raw)
Related papers
Isolation and Characterization of Antitumor Alkaloid from Poppy Capsules (Papaver somniferum)
Journal of Chemistry, 2013
Noscapine, a phthalideisoquinoline alkaloid derived from opium, has been used in the treatment of various cancer types. Its lowtoxicity pro�le has increased attention to this alkaloid. �ith regard to increasing demand for this compound, we developed a new method for isolation of noscapine from dried capsules of Papaver somniferum. Noscapine was successfully isolated from poppy capsules for the �rst time and the purity of the isolated compound was determined to be over 99.59% by HPLC analysis. e structure of noscapine was con�rmed by 1 H NMR, 13 C NMR, FT-IR, elemental analysis, and HR-ESI-MS methods.
Journal of Drug Delivery and Therapeutics
Papaver somniferum commonly known as Khashkhash /Afyon, belongs to family Papaveraceae. It is one of those traditional plants, which have a long history of usage as medicine. The opium poppy (Papaver somniferum) produces some of the most widely used medicinal alkaloids like morphine, codeine, thebain and porphyroxine which are the most important component of this plant. Apart from these alkaloids, opium poppy produces approximately eighty alkaloids belonging to various tetrahydrobenzylisoquinolinederived classes. It has been known for over a century that morphinan alkaloids accumulate in the latex of opium poppy. According to Unani literature, it possesses most important theurapeutic values as modern literature and research studies also prove its therapeutical importance. It is used as analgesic, narcotic, sedative, stimulant as well as nutritive, etc. It is also useful in headache, cough, insomnia, cardiac asthma, and biliary colic. In this paper we have provide a review on habitat...
Phytochemical, GC-MS and FT-IR Analysis of Papaver somniferum L
IP innovative publication pvt. ltd, 2019
The present study was aimed to analysis of bioactive constituents of Papaver somniferum (Poppy seed). The ethanol extract of the seeds were subjected to Phytochemical Screening, Gas chromatography- mass spectroscopic (GC-MS) and Fourier transform infrared spectroscopy (FTIR) analysis. GC-MS analysis of the seeds was performed using a Scion 436- GC Bruker model nd Interpretation on mass spectrum GC-MS was conducted using the database of National Institute Standard and Technology (NIST) and IR spectrum was recorded in spectrophotometer (Shimadzu, IR Affinity1, Japan). Phytochemical screening for seeds extracts indicated the presence of various secondary metabolites like Alkaloid, Cardiac Glycosides, Flavonoid, Phytosterols and Terpenoids. GC-MS analysis of compounds with totally, Thirty Nine volatile compounds major chemical compounds were identified, such as 9-Octadecynoic acid(30.72%), 9-Tetradecen- 1-ol, acetate, (E)- (24.02%), 9,12-Octadecadienoic acid, methylester, (E,E)- (7.82%), cis-9,10-Epoxyoctadecan-1-ol (7.43%) and Undec- 10-ynoic acid(4.36%). FT-IR analysis of peak values with various functional compounds such as alcohols, phenols, carboxylic acids, aldehydes, amides, amino acids, anhydrides, esters, ketones, Unsaturated aliphatics, aromatics, unsaturated heterocycles, amines, Nitro compound, Alkanes, alkenes, sugars,Sulphur, phosphorus, and fluorine compounds. The present results concluded that the phytochemicals was observed in ethanol extract which revealed that the Papaver somniferum (Poppy seed) is potential use in different fields namely medical and pharmaceuticals and greatly valuable in medicinal practice for the treatment of several human aliments. Keywords: GC-MS, FT-IR, Papaver somniferum L and NIST.
African Journal of Traditional, Complementary and Alternative Medicines, 2016
Background: The purpose of this study is to determine the effect of in vitro anticancer activity and cytotoxicity of 13 Papaver alkaloids (amurine, armepavine, berberine, isocorydine, isothebaine, macranthine, mecambrine, mecambridine, narkotine, orientalidine, oripavine, salutaridine and thebaine) against the human cervical cancer cell line (HeLa) compared to the normal African green monkey kidney epithelial cell line (Vero) using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Materials and Methods: The Vero and HeLa cell lines were treated with various concentrations (1-300 μg/mL) of alkaloids for 48 h. Values for cytotoxicity measured by MTT assay were expressed as the concentration that causes a 50% decrease in cell viability (IC50) (μg/mL). Results: Berberine and macranthine were the most active alkaloids. Salutaridine exhibited no cytotoxic activity against two types of cell lines. Dose-dependent studies presented IC50 of 12.08 μg/mL and IC50 of 71.14 μg/mL for berberine and IC50 of 24.16 μg/mL and IC50 of >300 μg/mL for macranthine on the HeLa cells and the Vero cells respectively. Conclusion: The degree of selectivity of the compounds can be expressed by its Selectivity Index (SI) value. High SI value (>2) of a compound gives a selective toxicity towards cancer cells (SI = IC50 for normal cells/IC50 for cancer cells). Two alkaloids showed significant SI values, which are 12.42 for macranthine and 5.89 for berberine. Hence, macranthine and berberine display potential to be further exploited in the discovery and development of new anticancer agents.
Content of alkaloids in ornamental Papaver somniferum L. cultivars growing in Estonia
Proceedings of the Estonian Academy of Sciences
The alkaloid content in industrial opium poppies (Papaver somniferum L.) has been studied by numerous authors, but not in ornamental cultivars.The aim of the study was to investigate the content of morphine, codeine, and papaverine in P. somniferum cultivars by high performance liquid chromatography (HPLC) and capillary electrophoresis (CE). The studied poppy capsules were either grown from the ornamental poppy seeds purchased from different vendors or were unknown cultivars from home gardens in Estonia, cultivated as ornamental poppies. All dried capsules samples (n = 34) contained on average 362 mg/100g of morphine, 25 mg/100g of codeine, and 29 mg/100g of papaverine as determined by CE. The content of morphine determined by HPLC was 152-676 mg/100g, which is a remarkably high concentration allowing potential illegal use of ornamental poppies. Both analytical methods demonstrated similar results. Hence, CE is a suitable alternative to HPLC for quantitative analysis of opium alkaloids as it simplifies the sample preparation procedure.
Cytotoxic Activity of Alkaloids from Papaver rhoeas growing in Lebanon
2016
This paper represents the phytochemical properties of Lebanese Papaver rhoeas, from which five protopine alkaloids are isolated, namely; stylopine (1), canadine (2), sinactine (3), berberine (4), and epiberberine (5). This is the first report for the isolation of epiberberine (5) from the genus Papaver and canadine (2) from Papaver rhoeas, suggesting a new chemotype of Papaver rhoeas growing in Lebanon. The cytotoxic activity of the total ethanolic extract and the isolated alkaloids were determined by MTT assay on human colon cancer cells (HCT116), breast cancer cells (MCF7), human keratinocyte cell line (HaCaT), and non-cancerous colon cells (NCM460). The compounds showed dose-dependent inhibitory effect with highest activity for compound 4 against all cell lines. The activity of the alkaloids varied between the various cell lines indicating cell type specificity and suggesting different cell-compound interactions. IC50 values on normal cells was higher than cancer cell lines (>...
Alkaloids of Papaver libanoticum and their Cytotoxic Activity
Records of Natural Products
Papaver libanoticum (family Papaveraceae) is an endemic Lebanese plant that has not been investigated before. The plant extract was found to possess a potent opioid analgesic activity with characteristic profile that suggest its potential use for the treatment of opioid abuse and withdrawal symptoms. The present study aims at exploring the cytotoxic activities of the plant extract and the isolated alkaloidal compounds. Chromatographic separation of Papaver libanoticum extract resulted in the isolation of; dehydroremerine (1), roemerine (2), berberine (3), alborine (4), remrefidine (5), and mecambrine (6). Their chemical structures were determined based on different recorded spectroscopic data in addition to comparison with those published in literature. The cytotoxic activity of the plant extract and its alkaloids (2-5) were determined by MTT assay on human breast cancer (MCF7) and human colon cancer cells (HCT116). The results indicated that berberine exhibited the highest inhibitory effect against both cell lines with IC50 of 22 and 106 µM on MCF7 and HCT116 cells, respectively. Other compounds (including the crude extract) showed different dose-dependent inhibitory effects on the two cell lines indicating cell type specificity and suggesting different cell-alkaloid interactions. Further studies are needed to explore the structure-activity relationships (SAR) of alkaloids derived from P. libanoticum.
The aim of this work was to investigate the phytochemical and anti-proliferative activity in vitro of the aerial parts of lotus peregrinus L. The preliminary phytochemical analysis of the aerial parts of lotus peregrinus L. (Family; Fabacea) revealed the presence of phenols, flavonoids, terpenoids, steroids, tannins and carbohydrates. Protein amino acids analysis showed the presence of 17 amino acids as protein amino acids. Aspartic acid (8.526 mg/g) and Glutamic acid (6.069 mg/g) represented as the major components of protein amino acids, respectively. Combined and free sugars analysis showed the presence of 12 free sugars. L-Rhaminose (21.9136) and Glucuronic (21.656 %) represented as the major free sugars, meanwhile the Xylose (29.425%); followed by Glucuronic (27.899%) represented as the major combined sugars. Twelve fatty acids were estimated; the highest percentage was that of Linoleic acid (18.489). Fourteen known hydrocarbon were found, the highest percentage was that of Eicosane (8.027%) in addition to three sterols; the highest percentage was that of Stigmasterol. two triterpene: alpha-Amyrin and lupeol in addition to one acyclic diterpene; Neophytadiene were detected. Anti-proliferative activity was carried out on the successive extracts (diethyl-ether, chloroform, ethyl acetate and ethanol 70% extracts) of lotus peregrinus L aerial parts to evaluate the Anti-proliferative properties. Diethyl ether was found to be potential anti-proliferative extract and promising natural agent. The chemical composition of the diethyl ethyl extracts can be useful in the chemosystematics of this species. Further studies will be needed to clarify the exact mechanism of Lotus peregrinus L. most active extracts as anti-cancer agent.
2012
The free radical generation is related to the oxidation process in biological systems as well as in foods. It was found that oxidation is affected by antioxidants that can act as radical scavengers. Objective of the present work was to study the free radical scavenging capacity of opium poppy (Papaver somniferum L.) extract by using the DPPH test and to verify the suitability of the micellar electrokinetic capillary chromatography (MEKC) technique for analytical assessment and determination of three major poppy alkaloids (thebaine, morphine and papaverine). Because of its generally high separation efficiency, the MEKC is successfully used for analytical evaluation of biologically active substances usually without special claims for sample preparation. The results of DPPH test have shown that poppy contains components capable of terminating free radicals. We have confirmed that nature of the solvent used for the electrophoretic medium in MEKC has a strong influence on the separation efficiency. In our experiments, the most effective solvent was mixture of water to acetonitrile (ratio 4:6).