Preliminary Phytochemical Screening and Antimicrobial Studies of Aqueous and Alcoholic Extracts of Mangifera Indica (Anacardiaceace) Stem Bark (original) (raw)
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In vitro antibacterial activity of crude leaf extracts of Mangifera indica Linn
The active components of leaves of Mangifera indica L. were extracted using cold water and organic solvents (acetone and methanol) and were tested against Staphylococcus aureus, Streptococcus pyogenase, Streptococcus pneumoniae, Bacillus cereus, Escherichia coli, Pseudomonas aerugenosa, Proteus mirabilis, Salmonella typhi and Shigella flexnerri using the agar well (cup plate) diffusion method. Both the acetone and methanol extracts inhibited the growth of gram positive bacteria, with acetone extract exerting more activities on all the gram positive bacteria with zone of inhibition between 15 - 16 mm, and a gram negative bacterium S. typhi (14 mm) at 250 mg/ml. Whereas, water extract was not active on any of the bacterial pathogens tested at any of the concentration of the extract used. The activities of the plant extracts on the inhibited pathogens using the zone of inhibition were not as effective as the standard commercial antibacterial disks of gentamicin and erythromycin (t = 2.23, p < 0.05). Increased temperature (60 and 100°C for 1 h) had a multiplier effect on the activity of the extracts, but alkaline pH decreased the activity. Preliminary phytochemical analysis revealed the presence of tannins, glycosides, saponins and phenols. The MIC and MBC of the extracts was in the range of 12.5 - 75 and 25 - 175 mg/ml respectively. There is a basis for the traditional use of the plant as a local health remedy. Key words: Antibacterial activity, MIC, MBC, gram-positive bacteria, gram-negative bacteria, Mangifera indica, extract.
Phytochemical and Antimicrobial Properties of Mangifera indica Leaf Extracts
2018
There have been reports of increasing development of drug resistance among human pathogens as well as undesirable side effects of certain antimicrobial agents.It is therefore necessary to search for new agents that are better, cheaper and without side effects for treating infectious diseases especially in developing countries. In this study, phytochemical composition and antimicrobial activities of aqueous and ethanolic extracts of leaves of Mangifera indica were investigated. Standard methods were employed to screen for the phytochemicals. Agar well diffusion method was used to determine the antimicrobial effects of aqueous and ethanolic extracts of M. indica leaves against seven different clinical isolates namely:Stapylococcus aureus, Micrococcus virians, M. leteus, Escherichia coli, Klebsellia pneumoniae, Pseudomonas aeruginosa and a fungus, Candida albicans. Phytochemical screening showed the presence of active pharmacological components such as tannins, saponins, cardiac glycos...
Antibacterial activity of Mangifera indica (L.)
African Journal of Ecology, 2007
Leaf extracts of Mangifera indica (L.), a medicinal and horticultural plant were investigated for antibacterial activity against Staphylococcus aureus, Esherichia coli and Pseudomonas aeruginosa. Using the agar-well diffusion and the gradient serial dilution methods the extracts showed weak antibacterial activity against the study organisms compared with the positive control (gentamycin). The ethanolic extract was most active with minimum inhibitory concentration ranging from 5481.0 to 43750.0 lg ml )1 . Chemical tests showed the presence of saponins, steroids and triterpenoids in the ether fraction, alkaloids, anthracenocides, coumarins, flavonones, reducing sugars, catechol and gallic tannins, saponins, steroids and triterpenoids in the ethanolic portion and anthracenocides, flavonones, reducing sugars in the aqueous fraction of the plant extract. These results shows that leaf extracts of M. indica possess some antibacterial activity that could be the basis for their medicinal use in Uganda.
Phytochemical Screening and Antibacterial Activity of Mangifera indica Extracts
UMYU Journal of Microbiology Research (UJMR), 2016
Mangifera indica leaves are used for the treatments of various ailments in folklore medicine. This research was aimed to determine phytochemical composition and antibacterial activity of leaves extracts of Mangifera indica. Powdered leaves of Mangifera indica were extracted with water, ethanol and chloroform solvents via percolation method. The extracts were tested for antibacterial activity against clinical isolates of Salmonella Typhi, Salmonella Paratyphi A and Salmonella Paratyphi B using aga rwell diffusion method. The extracts were further subjected to phytochemical tests for the presence of secondary metabolites using standard procedures. Results of the sensitivity test showed that highest zone of inhibition was observed in ethanolic extract with 13mm for S. Paratyphi A, 11mm for S. Paratyphi B, and 10mm for S. Typhi, followed by aqueous extract with 11mm for S. Paratyphi B and 10mm for S. Typhi. S. Paratyphi A, was resistant to both aqueous and chloroform extract of M. indica while S. Paratyphi B was only resistant to chloroform extract. Results of phytochemical screening indicated the presence of alkaloids, flavonoids, steroids, tannins and phenols.
Medicinal Efficacy of Methanol and Ethanol Crude Extracts of Mangifera indica Leaf
Journal of Microbiology Research, 2014
The aim of this study was to determine the antibacterial activity of methanol and ethanol leaf extracts of Mangifera indica against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Streptococcus pneumoniae, and Klebsiella pneumoniae. Antibacterial activity of the methanol and ethanol leave extracts of M. indica was determined by the agar well diffusion method at a concentration of 100 mg/ml while minimum inhibitory concentration (MIC) was determined by the agar dilution technique. The results obtained in this study showed that the ethanol and methanol crude extracts of M. indica leafhad antibacterial activities and inhibited the test pathogens substantially. However, methanol extract produced better zones of inhibition than the ethanol extracts. The zones of inhibition of both extracts ranged between 10-16 mm. Minimum inhibitory concentration (MIC) of the ethanol and methanol M. indica leaf was determined at 100 mg/ml and 50 mg/ml against all test isolates. In conclusion, this preliminary study has shown that M. indica possess potent antibacterial activity and this underscores the reason why the plant is used to meet a number of primary health care needs in most rural Nigerian communities.
Phytochemical Screening, and Antibacterial and Antioxidant Activities of Mangifera indica L. Leaves
Horticulturae
The bio-constituents of medicinal plants are greatly influenced by the environmental conditions and growing seasons. This study aimed to uncover the presence of different metabolites and to investigate the biological properties of the leaves of Mangifera indica during summer and winter seasons. The extract of M. indica leaves for summer and winter using different solvent extracts (hexane, chloroform, and methanol) showed the presence of phenols, flavonoids, tannins, terpenoids, alkaloids, phytosterol, saponins, steroids, and carbohydrates. Antibacterial activity of the methanolic leaf extracts for summer and winter were evaluated against the bacterial species Staphylococcus aureus (ATCC 43300) and Escherichia coli (ATCC 25922). For S. aureus (ATTC 43300), the summer crude extract displayed lower antibacterial activity than the control streptomycin, with zones of inhibition of 14.17 and 16.67 mm, respectively. Winter extracts had a zone of inhibition of 12 mm, while streptomycin had ...
The cold water, hot water, methanol suspension, Methanol column & methanol soxhelt extract of Mangifera indica peel powder was subjected to antimicrobial bioassay. Antibacterial activity was assayed against three pathogens namely Pseudomonas aeruginosa.E.coli and S.aureus for aqueous and methanolic extract of dry powder of M.indica peel. Among all hot water extract showed Maximum activity against S.aureus with 2.20 cm of zone of inhibition. Methanol suspension extract was best giving large zone of inhibitation against all the pathogens. The MIC of cold water was 4.28 mg/ml against Pseudomonas aeruginosa followed by methanol column was 10.71 mg/ml against E.Coli and S.aureus .
Natural Product Communications
Ethnobotanic surveys have revealed the use of Mangifera indica L. (Anacardiaceae) bark for the treatment of infectious diarrhea. The essential oil of M. indica bark is described for the first time for its chemical composition, radical scavenging activity (DPPH method) and antimicrobial properties. The total phenols content of its water and ethanol bark extracts as well as their radical scavenging and antimicrobial properties were also evaluated. Four commercial plant extracts were also studied for a comparison purpose. The antimicrobial activities were measured for all samples against three Gram (-): Escherichia coli, Salmonella enteritidis, Shigella and two Gram (+): Staphylococcus aureus and Bacillus cereus bacteria. The M. indica bark essential oil was characterized by the association of two major sesquiterpenes: ( E)-β-caryophyllene (60.3%) and α-humulene (36.7%). It presented the lowest ratio of concentration to inhibition zone diameter on all the strains. The aqueous and ethan...