Fabrication of ultrafine powder from eri silk through attritor and jet milling (original) (raw)
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International Journal of Molecular Sciences, 2021
Silk fibroin has a high potential for use in several approaches for technological and biomedical applications. However, industrial production has been difficult to date due to the lengthy manufacturing process. Thus, this work investigates a novel procedure for the isolation of non-degraded regenerated silk fibroin that significantly reduces the processing time from 52 h for the standard methods to only 4 h. The replacement of the standard degumming protocol by repeated short-term microwave treatments enabled the generation of non-degraded degummed silk fibroin. Subsequently, a ZnCl2 solution was used to completely solubilize the degummed fibroin at only 45 °C with an incubation time of only 1 h. Desalting was performed by gel filtration. Based on these modifications, it was possible to generate a cytocompatible aqueous silk fibroin solution from degummed silk within only 4 h, thus shortening the total process time by 48 h without degrading the quality of the isolated silk fibroin s...
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Silk fibroin protein derived from Bombyx Mori silk cocoon is a natural polymer that widely used for application in tissue engineering. Silk Fibroin has remarkable properties that demonstrates biocompatibility, biodegradable and great mechanical properties. In this present study, regenerated silk fibroin can be obtained by multistep preparation which are degumming and solubilisation process. The small pieces of regenerated silk fibers were cut and then blended with Polyvinylalcohol (PVA) solution to enhance the mechanical property of scaffold. Electrospinning capable to fabricate scaffold with the high surface area. Result showed that by increased of concentration of solution silk/PVA, voltage and flowrate, more fibers were produced in the film. A thin white film was produced at film of silk/PVA at 14% (w/v) without addition of chloroform. Scanning Electron Microscope (SEM) investigated the morphology of silk and revealed that applied voltage of 15kV during electrospinning has narrow...