Effect of the Concentration and the Type of Dispersant on the Synthesis of Copper Oxide Nanoparticles and Their Potential Antimicrobial Applications (original) (raw)
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Characterisation of copper oxide nanoparticles for antimicrobial applications
International journal of …, 2009
Copper oxide (CuO) nanoparticles were characterised and investigated with respect to potential antimicrobial applications. It was found that nanoscaled CuO, generated by thermal plasma technology, contains traces of pure Cu and Cu 2 O nanoparticles. Transmission electron microscopy (TEM) demonstrated particle sizes in the range 20-95 nm. TEM energy dispersive spectroscopy gave the ratio of copper to oxygen elements as 54.18% to 45.26%. The mean surface area was determined as 15.69 m 2 /g by Brunau-Emmet-Teller (BET) analysis. CuO nanoparticles in suspension showed activity against a range of bacterial pathogens, including meticillin-resistant Staphylococcus aureus (MRSA) and Escherichia coli, with minimum bactericidal concentrations (MBCs) ranging from 100 g/mL to 5000 g/mL. The ability of CuO nanoparticles to reduce bacterial populations to zero was enhanced in the presence of sub-MBC concentrations of silver nanoparticles. Studies of CuO nanoparticles incorporated into polymers suggest release of ions may be required for optimum killing.
Synthesis, Characterization, and Antimicrobial Activity of Copper Oxide Nanoparticles
Journal of Nanomaterials, 2014
We studied the structural and antimicrobial properties of copper oxide nanoparticles (CuO NPs) synthesized by a very simple precipitation technique. Copper (II) acetate was used as a precursor and sodium hydroxide as a reducing agent. X-ray diffraction patter (XRD) pattern showed the crystalline nature of CuO NPs. Field emission scanning electron microscope (FESEM) and field emission transmission electron microscope (FETEM) demonstrated the morphology of CuO NPs. The average diameter of CuO NPs calculated by TEM and XRD was around 23 nm. Energy dispersive X-ray spectroscopy (EDS) spectrum and XRD pattern suggested that prepared CuO NPs were highly pure. CuO NPs showed excellent antimicrobial activity against various bacterial strains (Escherichia coli,Pseudomonas aeruginosa,Klebsiella pneumonia,Enterococcus faecalis,Shigella flexneri,Salmonella typhimurium,Proteus vulgaris,andStaphylococcus aureus). Moreover,E. coliandE. faecalisexhibited the highest sensitivity to CuO NPs whileK. p...
Nano Biomedicine and Engineering
The antibacterial activity of various nanoparticles is gaining increasing interest due to its potential medical applications. In this work, we presented the synthesis of copper oxide nanoparticles prepared by chemical reduction from aqueous solutions of copper sulfate (CuSO 4) with sodium borohydride (NaBH 4) and hydrazine hydrate (N 2 H 4) as reductant and polyvinylpyrrolidone (PVP) as stabilizer. The X-ray diffraction spectra showed the formation of tenorite (CuO) and cuprite (Cu 2 O) nanoparticles when different ratios of CuSO 4 /NaBH 4 and CuSO 4 /N 2 H 4 were used. Photographs obtained by transmission electron microscopy (TEM) showed agglomerates of grains with a narrow size distribution (from 20 to 70 nm), whereas the radii of the individual particles were between 2 and 20 nm. Smaller nanoparticles and narrower particle size distributions were obtained when NaBH 4 was used. The results of antibacterial activity using the Kirby-Bauer method showed that nanoparticles obtained with NaBH 4 presented a reasonable bactericidal activity. Pseudomonas aureginosa and Staphylococcus aureus were more susceptible to the particle size than Escherichia coli. In addition, with small amounts of Cu 2 O in samples of CuO nanoparticles, the antibacterial susceptibility against Pseudomonas aureginosa was improved. Finally, nanoparticles of CuO incorporated into cotton by applying ultrasound waves remained impregnated after five washes.
CuO NANOPARTICLES: SYNTHESIS, CHARACTERIZATION AND THEIR BACTERICIDAL EFFICACY
International Journal of Applied Pharmaceutics, 2017
Objective: In the present study copper oxide (CuO) nanoparticles were synthesized and characterized. The antibacterial activity of CuO nanoparticles was carried out against Escherichia coli, Proteus vulgaris, Staphylococcus aureus and Streptococcus mutans. Methods: The synthesis was carried out by coprecipitation method using copper sulfate and sodium hydroxide as precursors. The synthesized copper oxide nanoparticles were characterized by using X-ray diffraction (XRD), Fourier transforms infrared spectroscopy (FT-IR), UV-vis spectroscopy and scanning electron microscope (SEM) with
Synthesis, Characterization and Antibacterial Activity of Cuo Nanoparticles
International Journal of Applied Pharmaceutics, 2019
Objective: The present study was done to see the effect of biologically synthesized CuO-NPs (Copperoxide nanoparticles) on the growth of bacterial strains. Methods: Physico-chemical characterization of CuO-NPs was done by UV-Vis-spectrophotometer, XRD, FE-SEM, and EDS. The disc plate diffusion assay was used to evaluate the anti-bacterial effect of CuNPs. Results: This study has shown a promising anti-bacterial activity of biosynthesized CuO-NPs at different concentrations ranging from 10 to 100 µg/ml against Escherichia coli and Staphylococcus aureus bacteria. Conclusion: Nanoparticles (NPs) are small size particles between range 1 to 100 nm which expand their physical and chemical properties due to high surface area. The present study reveals that there may be possible utilization of biosynthesized CuO NPs for the treatment of bacterial infectious disease in near future.
International Journal of Zoology and Applied Biosciences, 2022
This work was carried out to investigate the antibacterial activity of CuO nanoparticles (CuONPs), which were produced by synthetic methods of the precipitation technique of the precursor material copper acetate and sodium hydroxide as reducing agents. The purity of the nanoparticles was confirmed by various characterizations. Techniques including X-ray diffraction, Scanning Electron Microscopy (SEM), Ultraviolet (UV), EDAX. The antibacterial activity of nanoparticles was examined with various microorganisms from Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Klebsiella pneumonia.
International Research Journal of Pharmacy, 2017
Copper oxide (CuO) nanoparticles are known as multifunctional inorganic nanoparticles with a wide range of applications. This work mainly concentrated on the comparative study of antibacterial properties of CuO nanoparticles using two different methods. The green CuO nanoparticles were synthesized from Cajanus Cajan plant leaf extract. The CuO nanoparticles were visually confirmed by the change of color after addition of leaf extract into the copper acetate solution. The synthesized CuO nanoparticles were also characterized by using UV-Vis analysis, Fourier Transform Infrared analysis (FTIR), and X-ray diffraction analysis (XRD), Field Emission Scanning Electron Microscopy (FESEM) Energy Dispersive X-Ray (EDX) analysis. From the results, it is suggested that bio-CuO nanoparticles show more enhanced biocide activity against various human pathogens when compared to chemical CuO nanoparticles.
Asian Journal of Pharmaceutical and Clinical Research
Objective: To determine antimicrobial efficacy of copper oxide nanoparticles (CuO NPs) against Streptococcus sp. and Staphylococcus sp. Methods: CuO NPs were synthesized using chemical precipitation method. The reducing agent, 0.1 M NaOH, was used along with 100 mM CuSO4 precursor for the synthesis of CuO NPs. The characterization of CuO NPs was done by ultraviolet-visible spectroscopy and scanning electron microscopy (SEM) to study optical and morphological characteristics, correspondingly. The identification of bacterial cultures was done through microscopic and biochemical studies. Antibacterial efficacy of CuO NPs was determined against Streptococcus sp. and Staphylococcus sp. by qualitative and quantitative methods through anti-well diffusion assay and broth dilution method, respectively. Results: The absorption spectrum and band gap were found to be at 260 nm and 4.77 eV, respectively. The SEM image of CuO NPs shows cluster of nanostructures having width of individual clusters...
SYNTHESIS, CHARACTERIZATION AND ANTIBACTERIAL ACTIVITY OF CUO NANOPARTICLES Original Article
International Journal of Applied Pharmaceutics, 2020
Objective: The present study was done to see the effect of biologically synthesized CuO-NPs (Copperoxide nanoparticles) on the growth of bacterial strains. Methods: Physico-chemical characterization of CuO-NPs was done by UV-Vis-spectrophotometer, XRD, FE-SEM, and EDS. The disc plate diffusion assay was used to evaluate the anti-bacterial effect of CuNPs. Results: This study has shown a promising anti-bacterial activity of biosynthesized CuO-NPs at different concentrations ranging from 10 to 100 µg/ml against Escherichia coli and Staphylococcus aureus bacteria. Conclusion: Nanoparticles (NPs) are small size particles between range 1 to 100 nm which expand their physical and chemical properties due to high surface area. The present study reveals that there may be possible utilization of biosynthesized CuO NPs for the treatment of bacterial infectious disease in near future.
Green synthesis and morphology dependent antibacterial activity of copper oxide nanoparticles
Journal of Nanostructures, 2019
Cupper oxide nanoparticle (CuO-NPs) has been widely utilized in biomedical application due to their antibacterial function. It is well known that antibacterial characteristics of ma-terials could be controlled using the size, shape and composition of the particles. The aim of this paper is to green synthesis CuO-NPs with various morphologies, using Aloe Vera extract as reducing agent and investigate the effect of particle size and shape on the anti-bacterial properties. Results demonstrated the formation of pure CuO-NPs with crystallite size in range of 9-23 nm, depending on the precursor type and concentration as well as aging time. Furthermore, increasing the concentration of copper precursor from 6 mM to 1M altered the morphology from rod shape to spherical. We also examined the inhibitory effects of CuO-NPs toward the gram-negative bacterium, Escherichia coli and a gram-positive bacterium, Staphylococcus aureus cultures throughout a 24 hr period. Based on our data, while CuO-NPs...