Investigation of the presence of slime production, VanA gene and antiseptic resistance genes in Staphylococci isolated from bovine mastitis in Algeria (original) (raw)
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Detection of Some Virulence Factors in Staphylococci Isolated from Mastitic Cows and Ewes
Folia Veterinaria
About 150 million families around the world are engaged in milk production. However, inflammation of the mammary gland (mastitis) remains a major problem in dairy ruminants that affects the quality of milk worldwide. The aim of this study was the examination of udder health with detection of contagious and environmental pathogens causing mastitis in 960 and 940 dairy cows and ewes, respectively. The presence of selected virulence factors such as: the formation of haemolysis, gelatinase, biofilm, hydrolyse DNA, and resistance to antibiotics with detection of methicillin resistance gene (mecA), were determined in selected virulence factors associated with isolated staphylococci. These isolated staphylococci with selected virulence factors can have untoward effects on the severity of mastitis. The results of our study indicated that, in addition to the major udder pathogens (S. aureus, S. uberis, and S. agalactiae) causing mastitis, non-aureus staphylococci (NAS), is a major risk to da...
Alexandria Journal of Veterinary Sciences, 2015
Coagulase positive staphylococci (CPS) and coagulase negative staphylococci (CNS) are the most common cause of bovine intramammary infection. In this study out of 318 mastitic animals examined, 22.6 % are infected with staphylococcus aureus and 12.5% of mastitic cases were infected with CNS. Concerning subclinical mastitis cases, CPS was isolated from48.2% of cases while CNS was isolated from lower percentage 8.9%. High rates of resistance to antimicrobial agents was observed in CPS and CNS. PCR was performed to detect genes Sea, sec, see and tst on 19 staphylococci isolated. As shown in fig (1) 14 samples were positive for SEC toxin.. All samples were negative for sea, see and tst toxin. This study concluded the high significant role of staphylococci in bovine mastitis in Behera Governorate and its high resistance to antimicrobial. No specific virulence factor or combination of factors has been strongly associated with the severity of mastitis. The objective of this study is to investigate the role of staphylococci in bovine mastitis and to study some of its virulence genes.
Kafkas Universitesi Veteriner Fakultesi Dergisi, 2015
The study aimed to investigate the phenotypic and genotypic identification of in vitro antimicrobial susceptibility of 21 Staphylococci (10 Staphylococcus aureus and 11 Coagulase Negative Staphylococci) isolated from bovine mastitis to 12 antimicrobial drugs frequently using in veterinary medicine in Algeria. Isolates of staphylococci from bovine mastitis were tested for antibiotics with disc-diffusion method according to the National Committee for Clinical Laboratory Standards guidelines in the Mueller-Hinton agar, and resistant genes mecA, blaZ, aac-aph, ermA, ermC, tetK and tetM were detected by PCR. Staphylococci isolates showed high resistance to penicillin (95.23%), oxacillin (80.95%), clindamycine (80.95%), and erythromycin (76.19%) but, no resistance in all these strains was detected for gentamicin. Among 21 isolates of Staphylococci, 20 were found to be methicillin and multidrug resistant. Multidrug resistant strains exhibited several antibiogram patterns (antibiotic I to XIII). The distribution of antibiotic-resistant genes was mecA (100%) and tetM (100) followed by blaZ (42.85%). In the present study, the significant determination was the high prevalence of methicillin-resistant Staphylococci, which were resistant to multiple antibiotics. The finding of methicillin-resistant staphylococci from bovine mastitis is the first report in Algeria and revealed the status of resistant isolates in herd that might be helpful in treatment, controlling of resistant strains and for deciding culling of cows.
Distribution of antiseptic resistance genes in Staphylococcus spp. from bovine mastitis
Veterinární Medicína
The purpose of this study was the determination of antiseptic resistance genes (qacA/B and qacC) from staphylococcal mastitis in cattle in various regions of Turkey. In total, 283 isolates (Burdur: 36, Hatay: 47 and Van: 200) were studied, and the antiseptic resistance genes were detected using simplex PCR. The distribution of the qacA/B and qacC genes, mediating resistance against quaternary ammonium compounds, was found to vary among the different isolates. The qacA/B genes were found in three of the Burdur isolates, six of the Hatay isolates and seven of the Van isolates. The qacC gene was found in two of the Burdur isolates, none of the Hatay isolates and two of the Van isolates. The presence of these genes and transmission among Staphylococcus spp. strains may pose risks in the control of mastitis, as well as to public health.
Molecular Characterization of Staphylococci Isolated from Cattle with Mastitis
This study was carried out in order to investigate the occurrence of some virulence genes of Staphylococci isolated from cattle with mastitis. A total number of 133 milk samples (45 from clinical mastitis and 88 from subclinical mastitis) were collected from dairy cattle in Kafr El-Sheikh and EL Gharbia Governorates, Egypt. The samples were examined for the presence of Staphylococci by classical bacteriological methods and were further characterized geno-typically. A total of 41 Staphylococcus isolates were recovered from cattle with mastitis with an incidence of 30.8%. Among the isolates, 21(15.8%) of S. aureus [6 from clinical mastitis (13.3%) and 15 from subclinical mastitis (17%)] and 20 (15%) isolates of CNS [8 from clinical mastitis (17.7%) and 12 from subclinical mastitis (13.6%)] were identified phenotypically. All isolates were screened for the detection of binding protein A (spa-X), haemolysine type A (hla), Haemolysine type B (hlb), and toxic shock syndrome (tsst-1) by PCR. The obtained results revealed that the spa Xgene was detected in all Staphylococcus isolates recovered from subclinical mastitis while in clinical mastitis was detected with an incidence of 42.9%. Haemolysine type A was detected in clinical and subclinical mastitis with an incidence of 71.4% and 70% respectively, while haemolysine type B was detected in clinical and subclinical mastitis with an incidence of 28.5% and 40% respectively. Toxic shock syndrome was not detected in any of the isolates. The data in the study provided an overview on the distribution of some virulence genes related to Staphylococci isolated from cattle with mastitis in Egypt.
Indian Journal of Comparative Microbiology, Immunology and Infectious Diseases, 2016
To understand the prevalence and characteristics of Staphylococci causing goat mastitis in Algeria, mastitis milk samples were collected and cultured on both blood agar and mannitol salt 2 agar medium. Staphylococci isolates were identified by API-Staph gallery and tested for antibiotic susceptibility by disk diffusion method. DNA microarrays analysis was performed on S. aureus strains in order to detect virulence factors, including toxins, and to assign the isolates to theirs MLST clonal complexes. Overall, 86/266 (32.3%) goats suffered from mastitis, whose 32.5% (28/86) infected with Staphylococci. Most of strains were susceptible to many antibiotics, except for penicillin, tetracycline and fusidic acid, and the resistance incidence of which were 21.4%, 25%, and 10.7% respectively. Only twelve (42.8%) isolates were resistant to utmost two antibiotics and bi-drug resistance was associated to four Coagulase negative Staphylococci (CNS) strains. Nine different species were identified in CNS isolates while S. aureus strains were clustered within CC130-agr3-[lukF-P83, lukM+] (four isolates; 50%), CC6-agr1 (three isolates; 37.5%) and one agr4 non-typables cluster. Enterotoxins genes were found in 62.5% of the S. aureus strains. Of which sec, sel were the most prevalent (62.5%: n=5) followed by sea (12.5%: n=1). CC130 showed edinB, tst and tetK (2/4) genes and CC6 were positive to cna and fosB genes. The current investigation provide the data for prevalence of Staphylococci in goats in Tizi-Ouzou region and S. aureus characterization which will help in tracking evolution of epidemic strains and their control methods.
Pakistan Veterinary Journal, 2015
Received: Revised: Accepted: April 21, 2014 February 06, 2015 March 19, 2015 The objective of this study was to determine the antibiotic resistance in different Staphylococcus isolates using conventional and molecular methods. A total of 61 subclinical mastitis isolates of Staphylococci were evaluated for oxacillin, erythromycin, tetracycline, nitrocefin, and cefoxitin using the Kirby-Bauer disc diffusion method. The same isolates were also subjected to the multiplex PCR technique to detect mecA, femA and ermA, ermC, tetK, and tetM genes. Of the isolates, (Staphylococcus aureus, n=34 and coagulase negative Staphylococcus (CoNS), n=27) 26, 29, and 8 were resistant to erythromycin, tetracycline, and oxacillin, respectively in phenotypical evaluation. The genotypical evaluation indicated that of the strains, 34 carried erm genes in erythromycin-resistant strains and 10 carried tet genes in tetracycline-resistant strains. Agreement rates between genotypic and phenotypic evaluation for e...
Antibiotic susceptibility of Staphylococci isolated from bovine mastitis in Algeria
Journal of Advanced Veterinary and Animal Research, 2019
This work aimed to determine the resistance and/or the susceptibility to antibiotics of staphylococci isolated from cattle with mastitis in the North of Algeria. Materials and Methods: The disk diffusion method was carried out to reveal the antibiotic resistance in accordance to the National Committee for Clinical Laboratory Standards guidelines in the Mueller-Hinton agar. Results: Coagulase-negative Staphylococci (CNS) isolates showed more resistance to Cefoxitin, Amoxicillin + Clavulanic Acid, Vancomycin, Trimethoprime Sulfamethoxazole, Clindamycine, Neomycin, and Erythromycin than Coagulase-positive Staphylococci (CPS). CPS were more resistant to Penicillin and Tetracycline as compared to CNS strains; however, all these strains presented sensitivity to Gentamicin and neomycin. Conclusion: The Staphylococci showed high resistance to the beta-lactam antibiotics. As far as the authors know, these molecules are used with or without control in different protocols to prevent and cure the mastitis in Algeria.
Introduction: Bovine mastitis is a frequent cause of economic loss in dairy herds. Coagulase-negative staphylococci (CoNS) are increasing in importance as cause of bovine intramammary infection throughout the world in recent years. CoNS have been isolated from milk samples collected from cows with clinical and subclinical mastitis in several countries. Identification of mastitis pathogens is important when selecting appropriate antimicrobial therapy. Methodology: A total of 93 strains of Staphylococcus spp isolated from bovine mastitis in Argentina between 2010 and 2013 were identified by PCR-restriction fragment length polymorphism (PCR-RFLP) using the gap gene and were tested for the presence of blaZ and mecA genes by PCR and for the susceptibility to penicillin and cefoxitin by disk diffusion. Results: The most common CoNS species was S. chromogenes 46.2% (43/93), followed by S. devriesei 11.8% (11/93) and S. haemolyticus 9.7% (9/93). The blaZ gene was detected in 19 (20.4%), but only 16 (17.2%) isolates were resistant to penicillin; the mecA was detected in 6 (6.5%) isolates but only 4 (4.3) were resistant to cefoxitin. The 6 mecA-positive isolates showed oxacillin MICs ≥ 0.5 μg/ml. Discussion: CoNS are important minor mastitis pathogens and can be the cause of substantial economic losses. The presence of methicillin resistant isolates emphasizes the importance of identification of CoNS when an intramammary infection is present because of the potential risk of lateral transfer of resistant genes among staphylococcal species.
Advances in Animal and Veterinary Sciences, 2021
M astitis is defined as inflammations of the udder tissues resulting in sever reduction of milk yield and quality and causing significant economic losses in the cattle worldwide (Gezgen and Seker, 2016). Also, mastitis defined as an inflammatory condition associated with high somatic cell count (SCC) levels and drop in milk production (Taponen et al., 2007). Staphylococci have been reported to be the most common bacterial cause of subclinical mastitis (Pitkälä et al., 2004). However, CoNS are typical common bovine pathogen involved in subclinical mastitis, particularly in heifer, and are research Article Abstract | Coagulase-negative Staphylococci (CoNS) have emerged as an important Staphylococci species implicated in bovine mastitis in dairy herds. This study was conducted to determine the prevalence, antibiogram, and antibiotic resistance genes among coagulase-negative Staphylococci recovered from cattle suffer from subclinical mastitis in Egypt. A total of 110 (36.7%) milk samples collected from 300 lactating cows were positive for the California mastitis test. On the Mannitol Salt Agar medium, out of 110 subclinical mastitis samples 62 (56.36%) were identified as CoNS isolates. Antibiotic sensitivity test conducted against nine types of antibiotics for CoNS strains that exhibited high susceptibility to most of the tested antibiotics, with particularly resistance pattern to oxicillin (41.9 %), and 11 (17.7%) CoNS isolate exhibited multidrug resistance (MDR). A total of 15 randomly selected isolates were subjected for detection of antibiotic-resistant genes among CoNS. The results indicated that mecA (73.3%) was the most identified gene, followed by tetK (60%) and ermB (13.3%) genes, whereas no detection for blaZ and vanA genes. In conclusion, our results indicate the importance of the regular surveillance of phenotypic and genotypic profiles of CoNS, isolates to ensure effective control measures and minimize the evolution of MDR strains and recommended the applying of antibiotic sensitivity test before treatment or random selection of antibiotics in field cases to avoid the emerging of resistance phenomena.