Comparative analysis of 16S rRNA signature sequences of the genus Idiomarina and Idiomarina woesei sp. nov., a novel marine bacterium isolated from the Andaman Sea (original) (raw)

2014, Research in Microbiology

A Gram-negative, short rod, aerobic bacterium, designated W11 T , was isolated from seawater. Heterotrophic growth was observed at 10e45 C and pH 6e10. Optimal growth was observed at 30e37 C and pH 7e9. It can grow in the presence of 0.5e12% NaCl (w/v), and the optimal NaCl required for growth was 5e6%. 16S rRNA gene sequence similarity revealed that strain W11 T clustered within the radiation of the genus Idiomarina and showed 99.24% similarity with Idiomarina donghaiensis JCM 15533 T , 97.64% with Idiomarina marina JCM 15083 T , 97.37% with Idiomarina tainanensis JCM 15084 T and 97.16% with Idiomarina maritima JCM 15534 T. DNAeDNA similarities between strains W11 T with other closely related strains were below 70%. Polar lipids included a phosphatidylgylycerol, a diphosphatidylglycerol, a phosphatidylethanolamine, an unidentified phosopholipid, two unidentified aminolipids and two unidentified lipids. DNA G þ C content was 41.2 ± 0.1 mol%. Major fatty acids were iso-C 15:0 , iso-C 17:0 , iso-C 17:1 u9c, C 16:0 , iso-C 11:0 3OH and C 16:1 u7c/C 16:1 u7c. The isoprenoid ubiquinone was Q8. On the basis of the present polyphasic taxonomic study, strain W11 T is considered to represent a novel species of the genus Idiomarina, for which the name Idiomarina woesei sp. nov.