Immunomodulatory Effect of Novel Electrospun Nanofibers Loaded with Doxycycline as an Adjuvant Treatment in Periodontitis (original) (raw)
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Journal of Applied Oral …, 2010
OBJECTIVES: The aim of this study was to test the efficacy of a locally applied 8.5% nanostructured doxycycline (DOX) gel in preventing alveolar bone loss in experimental periodontal disease (EPD) in rats by using the tapping mode atomic force microscopy (AFM). MATERIAL AND METHODS: EPD was induced in 24 Wistar rats. Animals were treated with the doxycycline gel topically, immediately after EPD induction, and 3 times a day during 11 days. Four groups (n=6) were formed as follows: Naïve group (animals not subjected to EPD nor treated); non-treated (NT) group (animals subjected to EPD, but not treated); vehicle gel (VG) group (animals subjected to EPD and treated with topical gel vehicle); and DOX group (test group): animals subjected to EPD and treated with the 8.5% DOX gel. In order to investigate topographical changes in histological sections, a novel simple method was used for sample preparation, by etching sections from paraffin-embedded specimens with xylol. RESULTS: Comparing the AFM images, several grooves were observed on the surface of the alveolar bone and other periodontal structures in the NT and VG groups, with significantly greater depths when compared to the DOX group (p<0.05). CONCLUSIONS: Periodontal structures were brought into high relief confirming to be a simple and cost-effective method for AFM imaging with ultrastructural resolution. The doxycycline gel was able to afford periodontal surface preservation, with flatter grooves.
Egyptian Dental Journal, 2017
Background & Objective: Nanoparticles have received considerable attention in recent years. This study was conducted to compare between doxycycline loaded nanoparticles (DLNs) and conventional doxycycline gel (CDG) in management of chronic periodontitis (CP). Materials and Methods: This randomized, split mouth study was conducted on 40 sites among 20 patients with chronic periodontitis. The sites were randomly allocated into a control group, receiving scaling and root planing (SRP) plus CDG, and an experimental group, receiving SRP plus DLNs. Clinical parameters and levels of matrix metalloproteinase 1 (MMP1) were assessed at baseline, 1, 3 and 6 months post treatment. Bone density was also assessed at baseline and 6 months after treatment. Results: The results, following the use of both treatment gel formulas, showed a statistically significant reduction in all tested clinical parameters and levels of MMP1 at 1, 3 and 6 months post treatment along with significant increase in bone density after 6 months. However, DLNs treatment achieved a significant reduction not only in clinical parameters but also in MMP1 as compared to CDG treated group. In addition, The DLNs showed a significant bone formation as compared to CDG group. Conclusion: DLNs shows promise as adjunctive local therapy for management of patients with chronic periodontitis. Clinical Relevance: Scientific Rationale for Study: Evaluating the effect of doxycycline loaded nanoparticles gel and conventional doxycycline gel on treatment of chronic periodontitis. Principal Findings: Findings from this randomized, split mouth study of 40 sites among 20 patients with chronic periodontitis supported the potential of doxycycline loaded nanoparticles and conventional doxycycline gel for providing an effective therapy for chronic periodontitis, along with improving clinical parameter, bone density and decreasing matrix metalloproteinase 1. Practical Implications: Doxycycline loaded nanoparticles are proved to be more effective than conventional doxycycline gel as an adjunctive treatment of chronic periodontitis.
GLOBAL JOURNAL FOR RESEARCH ANALYSIS, 2020
Introduction: Various chemical agents such as nonsteroidal, anti-inflammatory drugs and antimicrobial agents has gained popularity in treatment of periodontal disease but simultaneously lead to condition such as drug resistance and drug allergy. Hence , the topical application of herbal agents such as propolis, aloevera, green tea extracts, Neem reduces the potency and effectiveness to prevent progression of periodontal disease. NanoBioFusion(NBF)gel contains the natural antioxidant power of propolis,vit C,vit E which allows the ultrafine antioxidant to surpase the moist intraoral environment to enter the cells and rejuvenate,revitalize,support,protect and optimize gum and soft oral tissue.Hence the present study is aimed to evaluate the clinical effect of locally delivered NBF gel as an adjunctive therapy to scaling and polishing in the treatment of Periodontitis. Materials and Methods: Chronic Periodontitis patients with 40 sites and probing pocket depth (PD) between 5 and 7 mm we...
Nanocomposite based drug delivery for periodontal disease
International journal of health sciences
Periodontal disease is the term used to describe some pathological conditions characterized by degeneration and inflammation of gums, periodontal ligaments, alveolar bone, and dental cementum. Periodontal disease involves destruction of alveolar bone around the teeth leading to defects or rather loss of the tooth if left untreated. This condition is caused by a chronic, mixed infection of Gram-negative and Gram-positive bacteria. Chlorhexidine (CHX) gluconate is an antiseptic, antifungal and bactericidal agent. CHX inhibits plaque formation by binding to anionic salivary glycoproteins and bacteria. Nano Hydroxyapatite (nHAP) is calcium phosphate in morphology and composition like the human hard tissues. Delivery of medications directly into the periodontal pockets alters the inflammatory response and reduces the pathogenic microbiota, an aspect which has attracted great interes. Result: The CHX Nanocomposites were non-toxic to human cells and promoted cell adhesion, proliferation, a...
Synthesis, Properties and Clinical Applications of Electrospun Nanomaterials in Periodontics
Electrospinning is a technique used to fabricate polymeric fibres of nanometre dimensions by applying high voltage difference to a syringe loaded with polymeric solution. This technique has enabled production of highquality tissue engineering scaffolds for application in periodontal regeneration and other biomedical applications. Technique depends on various parameters like voltage difference, jet speed, distance between the collector plate etc. The electro spun nanofibers possess superior physical, chemical, biological and mechanical properties which are noteworthy. The clinical application of electro spun nanofibers span across all fields of biomedical science which includes tissue engineering and regenerative medicine, diagnostics, drug delivery and wound healing. Of these specific interest lies in the field of periodontal regeneration. The aim of this review is to familiarize the concept of electrospinning and its vast applications in the field of periodontics for its better clinical use in dentistry.
AAPS PharmSciTech, 2024
Antibiotic administration is an adjacent therapy to guided tissue regeneration (GTR) in the management of periodontitis. This is due to the major role of pathogen biofilm in aggravating periodontal defects. This study aimed to fabricate a GTR membrane for sustained delivery of doxycycline hydrochloride (DOX) while having a space-maintaining function. The membranes were prepared using a polymeric blend of polycaprolactone/polyvinyl alcohol/chitosan by the electrospinning technique. The obtained membranes were characterized in terms of physicochemical and biological properties. Nanofibers showed a mean diameter in the submicron range of < 450 nm while having uniform randomly aligned morphology. The obtained membranes showed high strength and flexibility. A prolonged in vitro release profile during 68 h was observed for manufactured formulations. The prepared membranes showed a cell viability of > 70% at different DOX concentrations. The formulations possessed antimicrobial efficacy against common pathogens responsible for periodontitis. In vivo evaluation also showed prolonged release of DOX for 14 days. The histopathological evaluation confirmed the biocompatibility of the GTR membrane. In conclusion, the developed nanofibrous DOX-loaded GTR membranes may have beneficial characteristics in favour of both sustained antibiotic delivery and periodontal regeneration by space-maintaining function without causing any irritation and tissue damage.
Biomedicines, 2021
The purpose of this study was to prepare poly (D-L) lactide-co-glycolide (PLGA) and poly ε-caprolactone (PCL) nanofibers containing metronidazole and amoxicillin using an electrospinning process as intrapocket sustained-release drug delivery systems for the treatment of periodontal diseases. Scanning electron microscopy showed that the drug containing PLGA and PCL nanofibers produced from the electrospinning process was uniform and bead-free in morphology. The obtained nanofibers had a strong structure and resisted external tension according to the tensiometry results. The cytotoxicity results indicated acceptable cell viability (>80%). Quantification by high-performance liquid chromatography showed almost complete in vitro drug release between 7 and 9 days, whereas 14 days were required for complete drug release in vivo. No significant signs of irritation or inflammatory reaction were detected after three weeks of subcutaneous implantation of nanofibers in the animal models, thu...
Dental Materials Journal, 2020
The objective of this study was to develop electrospun polycaprolactone (PCL) membranes blended with hydroxyapatite (HA) and evaluate its potential in differentiating inflamed dental pulp stem/progenitor cells (IDPSCs) into odontoblasts. Electrospun nanofibrous membrane consisting of PCL blended with 10 wt% and 15 wt% of HA were fabricated and the characterization was done by Scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and contact angle analysis. Cytocompatibility, cell adhesion and odontogenic differentiation ability of the membranes were assessed by MTT, Live/ Dead, SEM/DAPI and qPCR studies. The mineral deposition ability of the membranes with IDPSCs was estimated by SEM-EDS. The SEM analysis revealed a nanofibrous texture with an average fiber diameter of 140 nm for PCL, 220 nm for PCL10%HA and 250 nm for PCL15%HA. Among the membranes tested, PCL10%HA favored positive cell attachments, upregulated expression of DSPP and ALP gene and higher Ca/P ratio compared to PCL and PCL15%HA.
Journal of Advanced Periodontology & Implant Dentistry
Background. The aim of this study was the clinical and laboratory evaluation of using polycaprolactone (PCL) nanofibers loaded with oxytetracycline hydrochloride (OTC) and zinc oxide (ZnO) as an adjunct to mechanical therapy in managing stage II grade A periodontitis patients concerning GCF lipocalin2- levels. Methods. Fifty stage II grade A periodontitis patients (27 women and 23 men, with an age range of 30‒60) were enrolled in the study. The patients were randomly assigned to five equal groups and treated with scaling and root planing (SRP), followed by local application of PCL nanofibers: group I: SRP + PCL loaded with OTC and ZnO, group II: SRP + PCL loaded with OTC, group III: SRP + PCL loaded with ZnO, group IV: SRP + unloaded PCL, and group V: SRP alone. Additionally, 10 healthy subjects with healthy periodontium (group VI) (age- and gender-matched) served as the negative control. Nanofibers were applied in the selected pockets of periodontitis patients in groups I to IV onc...