Revisiting Cytochemical Myeloperoxidase (MPO) Stain for Early Diagnosis of Acute Promyelocytic Leukemia (APML) in Areas with Limited Resources: An Experience from a Tertiary Care Center in India (original) (raw)

Blood, 2017

Abstract

Introduction- Acute Promyelocytic leukemia (APML) needs to be suspected early so that All-Trans Retinoic Acid (ATRA) therapy can be started as soon as possible. Flow cytometry shows absence of CD34 and HLADR in most cases and so can aid in diagnosis along with the usual morphological clues. However, it may have confusing patterns as in hypogranular APML and sometimes may be positive for HLA- DR and CD34. RT-PCR for detection of characteristic APML translocations (t(15;17),t(17;17) and others) is gold standard for APML diagnosis which is time consuming and often not possible especially in resource limited areas. Cytochemical MPO stain is a robust laboratory test that can be a valuable aid to morphology in early diagnosis of APML. In this study, the role of cytochemical MPO was evaluated for early diagnosis of APML. Method- This study included all the cases of APML that were diagnosed in a tertiary care center in India from May 2011 to May 2014. Cytochemical stains (MPO, Non- specific...

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