Sex Ratio of Calves Resulted from Artificial Insemination Implementation using Sexed Semen with Percoll Gradient Density Centrifugation Method in Ongole Crossbred Cows (original) (raw)
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IOP Conference Series: Earth and Environmental Science, 2019
The technology of artificial insemination is very popular to increase the population of cattle in Indonesia. This technology could be more valuable with sexing sperms in order to get the offspring with expected sex. The aim of the research is to know the proportion of male calves that was born from crossbred Ongolecows which were inseminated using double dosesemen sexed for Y sperm. The materials were 43 calves from Artificial Insemination Center Singosari. Twenty four calves was born from cows inseminated with non-sexed semen and nineteen calves with sexed semen. The percentage of sperm carrying X and Y chromosomes was performed by measuring the size of the sperm’s head by using a microscope. The result showed that the proportion of Y sperm in sexed semen was 80.79 %, while the X sperms was 19.21%. Furthermore, the percentages of the male calves that born from double dose sexed Y sperm was 78.95 %, while from the non sexing-semen was 21.05%. It can be conluded that the artificial i...
The Effect of the Use of Sexed Semen on Reproductive Traits and Sex Ratio of Black and White Calves
2015
Sperm sexing is a relatively new biotechnological procedure which offers us the possibility to choose the offspring sex. This method is widely used today in the insemination of the dairy cattle breeds and it is based on the difference in total DNA quantity between X and Y chromosome spermatozoa. In this research paper we have analysed the fertility, sex ratio and characteristics of female Black and White breed calves inseminated by the sexed sperm produced by 6 bulls on 6 commercial farms of the PK Belgrade. Conception rate was 44% with average gestation length of 274.9 days. Average calf birth weight was 36.75 kg. The stillbirths and twinning rates were 7.16 and 1.13%, respectively. Artificial insemination by sexed sperm assessed by c2 test highly significantly (P0.05) gestation length. Regardless the low conception rate, the application of sexed sperm in dairy cattle breeding is very significant since it enables the breeders to use the best replacement female animals what may resu...
Artificial Insemination using liquid sperm Filial Ongole Bull after sexed with different methods
Journal of Physics: Conference Series, 2019
The purpose of this study was to determine the success of artificial insemination (AI) using liquid sperm Filial Ongole Bulls after sexed by the percoll density gradient centrifugation (PDGC) and egg white sedimentation (EWS) sexed methods. The study materials were 135 cattle were inseminated using non sexed semen, PDGC and EWS sexed methods. Non-return rate (NRR) and the conception rate (CR) are observed. The research design used for the success of AI using chi square. The result of pregnancy examination at the age of 3 months found 21 head of pregnant cows from 45 cattle in AI with without sexed (control), 14 head of pregnant cattle from 45 cattle in the AI with EWS method, 18 head of pregnant cattle from 45 cattle in AI with PDGC method. The result showed that CR value using liquid sperm result of EWS, PDGC and without sexed were very significant (P <0.01). CR value is still higher sperm without sexed (46.67%) followed by PDGC (40%) and EWS (31.11%). The value of conception ra...
Review on process, advantages and disadvantage of artificial insemination in cattle
2018
The objective of this paper mainly aimed to review the AI and its advantage over natural breeding. Artificial insemination (AI) with semen collected from genetically superior sires is the most efficient and economical method for the genetic improvement of economically important traits in the beef industry. Semen used for artificial insemination is collected by using artificial vagina, electro-ejaculation, or massaging ampullae of the ductus difference through rectal wall. The objective of this paper is to review bull fertility assessment, semen collection, evaluation, processing, and insemination techniques in cow, and oestrus detection of cow and to review the advantages and disadvantages of AI as compared to Natural breeding. Once the semen is collected the semen is evaluated for its appearance, volume, motility, concentration, morphology, by macroscopic and microscopic tests. Semen is diluted to increase its volume and its lifespan of spermatozoa under cooling, freezing and ambie...
Effect of time of artificial insemination on embryo sex ratio in dairy cattle
Animal Reproduction Science, 2006
Prospective and retrospective study was conducted to evaluate and compare the sex ratio of calves following artificial insemination and natural mating. In the present study, animals that are inseminated from 8-18h from the onset of estrus were considered as early insemination; while animals inseminated >18h were also considered as late inseminated animals. Questionnaire survey, observation and secondary data were used to collect the information in the present study and out of the total number of calves (N=307) born by artificial insemination; 147(47.9%) animals were female and the rest 160 (52.1%) were male calves. whereas the number of animals calved by natural mating were 193 in which 107 of them were females and 86 were males and the sex ratio (female/male) was 55:45. Similarly, based on the findings of the evaluation and comparison of sex ratio conducted on farm type, the total number of animals from small scale dairy farms were 250 out of this 121 calves were females and 129 were males with this result the sex ratio (female/male) in small scale dairy farm were 48:52 and out of the total number of 250 calves recorded from large scale dairy farm 133 were females and the rest 117 animals were males and the sex ratio (female/male) was 53:47. The present result showed that the sex ratio of calves, by both the service type (AI or natural) and farm type (small or large scale), though numerically slight effect was appreciated, it was not statistically significant (P>0.05). On the other hand, of the total number calves (407) born following early (the first 8-18h) AI/bull service, 225 were females and the rest 182 were males and the sex ratio (female/ male) of this animals was 55:45, whereas out of the total number of 93 calves, that are calved through late insemination/service, 26 (28%) of them were female and 67 (72%) of them were males. This result showed that the sex ratio of calves were significantly affected (P<0.5) by the time of insemination in relative to estrus onset. In conclusion, the chance of female calves were increased when performing the AI/bull service within the first 8-18h from the onset of estrus, whereas delaying the AI time increases the probability of male calves.
Some Factors Affecting Pregnancy Rate During Artificial Insemination in Cattle
Annals of Tropical Medicine and Public Health, 2019
This study was conducted in the northern regions of Basra province / Iraq from March 2017 to February 2018. Nine-hundred cow age ranges between 2 to 5 years, weights from 200 to 400 kg and have crossbreeding and local breeds.Prior to initiating the artificial insemination, the rectal palpation of the cows was performed to determine the estrous phase and palpation the ovaries to determine the ovarian state and ovulation site. The cows was inseminated artificially using three different sources of frozen semen (Holland, Iranian and Iraqi) source with two different straw volume (0.5 or 0.25) ml., and during the insemination; the deposition of semen was performed in three different sites of female genitalia (uterine horn, uterine body and cervix).Depending on the source or origin of the frozen semen, the results showed there are no effect on successful insemination when use of frozen semen with a Holland, Iraqi or Iranian source, there are no significant differences at p < .05. (F = 1.1877).Depending on the size of the straw used (0.25 ml or 0.5 ml); the results showed there are no significant differences between the two volumes at (p < .05).Depending on the site of semen deposition within the female reproductive system; the results showed there are a significant differences at (p < .05) and (F = 8.73035). The highest percentage of successful inseminations showed when the deposition of semen in the uterine horn, compared to the deposition in the body of the uterus or cervix. Depending on the number of inseminations, the results showed that there are no significant differences between the study groups at (p < .05) and (F = 60.54751).The total percentages of successful inseminations in this study were 785/900 (87%), while the failed inseminations were 115/900 (12%).After third insemination; the cases were excluded and considered as a Repeated Breeders cows.
American Journal of Animal and Veterinary Sciences
One of the potencies of Madura cow genetics is its adaptation ability to the tropical climate. Yet in most cases, the reproduction efficiency of the Madura cows has not reached the optimum point. This research examines the use of double-dose artificial insemination compared to a singledose on Madura cows. The study was conducted in Daramista, Lenteng District, Sumenep Regency, Madura, East Java. This research utilized an experimental field method with purposive sampling on 100 Madura cows, with a body condition score of 3-4, aged about 2-6 years old. The treatments were divided into two groups, T0 and T1. T0 cows received single-dose artificial insemination treatment on the 8 th h after estrus and T1 cows received double-dose artificial insemination treatment on the 2 nd and 8 th h after estrus. The Singosari National Artificial Insemination Centre produced Madura bull frozen semen straws used in this research. Before inseminating, frozen semen straws were thawed at 28°C for 30 sec. This research observed some variables, including; Non-Return Rate (NRR1, NRR2, NRR3), Service per Conception (S/C), Conception Rate (CR), and estrus quality condition as supporting data. The researchers analyzed the data descriptively and through chi-square analysis. The result of the study showed that NRR1, NRR2, and NRR3 of T0 were 94, 84, and 70%, while NRR1, NRR2, and NRR3 T1 were 92, 86, and 82%. The CR of T0 was 64% compared to the CR of T1 at 76%. The double dose insemination method at different times on the 2 nd and 8 th h after estrus can increase the reproduction performance in Madura cows.
Sexed semen technology in cattle: A revolutionary technique in Indian dairy industry
Journal of Entomology and Zoology Studies, 2019
Desired sex either female or male which is produce from semen having X or Y bearing sperm is known as sexed semen. Sexed semen increase the genetic progress in a herd by increases the number of superior heifers and good male germplasm from elite bulls which is used for future breeding programme. Various methods are developed based on density gradient centrifugation or swim-up, sex specific antibodies, free flow electrophoresis and flow cytometry that efficiently separate bovine semen into fractions containing higher concentrations of X or Y chromosome bearing sperm. Flow cytometry is the only proven method for semen sexing to be commercially viable more than 90% accuracy to produce calves of desirable sex. Other methods for sex sorting of sperm (Albumin Gradient/ Percoll gradient/ Gradient swim down, Centrifugal counter current distribution, Free flow electrophoresis, Identification of H-Y antigen, Genetic approaches etc.) have also emerged though these techniques further needs fine tuning for commercial viability. This paper aimed to review the sexed semen methodology, utility of sex sorted semen, benefits and limitation of sex sorted semen.
Quality of Sexed Sperm of Bali Bull in Regional Artificial Insemination Center of Riau Province
Buletin Peternakan, 2019
This study aimed to evaluate the quality of frozen semen of Bali bull resulted from sexing procedure on calf or offspring production with desired sex. The tested sperm of Bali bull were collected from Bali bull raised at Regional Artificial Insemination Center of Riau Province (BIBD Riau). The study was carried out in 2 stages. The first stage was X and Y chromosome separation by albumin method. The extender used in the sexing procedure is trice citrate fructose and egg yolk. The second stage was mainly testing the sexed sperm collected in 60 Bali cow in Langkat Village, Bengkalis Regency. To determine the quality of post thawing frozen semen collected from the sexing procedure, the study evaluated motility, viability, mortality, abnormality and plasma membrane integrity of the spermatozoa. The pregnancy rate, calving rate, and birth accuracy of inseminated sexed sperm to offspring’ sex were also evaluated. The evaluation resulted in motility (66.3-75.3%), viability (70-78.5%), plas...
E3S Web of Conferences, 2022
The success of artificial insemination must be supported by a balance of nutrients for livestock. AI combined with sexing produces offspring according to wishes of the breeder. This study aims to determine the increase in concentration on the success of Artificial Insemination using frozen semen sexing. This research was conducted at the people's beef cattle farm, Sumber Pucung District, Malang Regency. The limousine crosser used was 30 cows. Cattle aged 1.8 – 5 years with BCS 3-5 (score 1-9) Treatment T0 : The usual feed given by farmers (Control). T1: Control+HQFS. Parameters NRR-1, NRR-2, Conception Rate (CR), Pregnancy Rate (PR). The additional feeding of concentrate did not significantly affect the reproductive parameters. The results showed that at T0 the results of NRR-1 and NRR-2 were 80% and 73.33%, respectively. while in T1 it was 73.3% and 66.67%. The results showed that the CR in both treatments was 26% and the results showed that the PR for T0 was 26% and for T1 was...