Extended Spectrum β-Lactamase Mobile Gene Encoded Variants CTX-M, TEM, SHV, AmPC and FOX among Serratia marcescens, Ile- Ife South Western Nigeria (original) (raw)
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Transplantation Proceedings, 2009
Background. Serratia marcescens is an important pathogen in hospital infections since organisms resistant to multiple antimicrobials pose a special threat particularly among transplant patients. The aim of this work was to assess the number of strains producing -lactamases with extended spectrum (ESBL) among S. marcescens isolated from our patients. Materials and Methods. We investigated S. marcescens isolated from 2005 to 2008 for ESBL. The phenotype methods were applied and additionally we chose strains for polymerase chain reactions using primers for the most popular types of ESBL. Results. Over the investigated time, 257 patients were infected with S. marcescens with 188 (73%) displaying an ESBL-positive phenotype. A Molecular analysis showed that most of them produced both CTX-M and TEM -lactamases. In the last year, the percentage of ESBL-producing strains decreased, but also in the last year, we isolated S. marcescens resistant to carbapenems from three patients. Conclusions. The CTX-M type of ESBL predominated among ESBLs produced by strains of S. marcescens. The appearance of strains resistant to carbapenems is alarming.
Iranian journal of microbiology, 2013
Nowadays, the presence of extended-spectrum β-lactamases (ESBLs) producing strains in Serratia genus causes the emergence of resistance to many antibiotics. So, the lack of proper diagnosis of ESBLs strains can lead to failure in the treatment. The objective of the present study was to investigate ESBLs production in Serratia strains isolated from the clinical blood samples in Shiraz, Iran. In this study, 39 Serratia strains isolated from the patients referred to Namazi Hospital, during a 2 year period were tested. The antimicrobial resistance of the isolates to 21 antibiotics was evaluated using Kirby-Bauer disk diffusion method. Combination disk method was used to determine the ESBL phenotype among the isolates. PCR was performed to investigate the presence of ESBL genes of SHV, OXA and TEM types. The lowest antibiotic resistance rates belonged to meropenem (7.69%) and imipenem (5.12%). Overall, positive ESBL phenotype was identified in 69% (n = 27) of the isolates, 70.37% (n = 19...
International Journal of Research in Pharmaceutical Sciences
Serratia marcescens is an important nosocomial pathogen that causes a variety of infections, especially urinary tract and bloodstream infections. The emergence and spread of multidrug-resistant Serratia marcescens producing extended-spectrum beta-lactamases is a threat to public health worldwide at present. Extended-spectrum β-lactamases (ESβLs) including TEM, SHV, and CTX-M are the predominant types that confer resistance to beta-lactam group of antibiotics. Many reports have been investigated ESBL-producing isolates of Enterobacteriaceae in Iraq. However, there are few studies concerned about ESβL-producing Serratia marcescens particularly, detection of ESBLs encoding genes. Therefore this study aimed to identify ESβLs encoding genes in Serratia marcescens isolates from a neonatal intensive care unit. Fifty isolates were identified phenotypically using the VITEK® 2 compact system. For confirming the identification of bacterial strains, molecular detection of housekeeping LuxS gene...
Serratia marcescens Isolated in 2005 From Clinical Specimens From Patients With Diminished Immunity
Transplantation Proceedings, 2007
Serratia marcescens is an important agent in hospital infections. The aim of this paper was to compare the resistance patterns of S. marcescens strains isolated during 1 year from patients of various wards of the Institute of Transplantology. The mechanisms of beta-lactam antibiotic resistance were of especial interest. We investigated the 81 strains of S. marcescens, isolated during 2005 from patients on 3 wards and 1 clinic of the Transplantation Institute. An unusually high resistance to most antibiotics was observed among S. marcescens strains. Extended spectrum beta-lactamases (ESBLs) were probably produced by 63.2% to 84.6% of strains, depending on the ward. Additionally, about 30% of them were probably derepressed AmpC producers. The patterns of resistance indicated that at least 2 resistant clones of S. marcescens spread among the patients. One of the clones demonstrated both ESBL and derepressed AmpC production and was susceptible only to carbapenems. The second, producing ESBL, was susceptible to pipercillin/ tazobactam and carbapenems. All investigated strains were resistant to nitrofurantoin. Strains of the second group were rarely susceptible to other antibiotics: aminoglycosides, ciprofloxacin, cotrimoxazole, or fosfomycin.
International Journal of Environmental Research and Public Health, 2021
Serratia marcescens (SM) is a Gram-negative bacterium that is frequently found in the environment. Since 1913, when its pathogenicity was first demonstrated, the number of infections caused by SM has increased. There is ample evidence that SM causes nosocomial infections in immunocompromised or critically ill patients admitted to the intensive care units (ICUs), but also in newborns admitted to neonatal ICUs (NICUs). In this study, we evaluated the possible genetic correlation by PFGE between clinical and environmental SM strains from NICU and ICU and compared the genetic profile of clinical strains with strains isolated from patients admitted to other wards of the same hospital. We found distinct clonally related groups of SM strains circulating among different wards of a large university hospital. In particular, the clonal relationship between clinical and environmental strains in NICU and ICU 1 was highlighted. The identification of clonal relationships between clinical and envir...
Outbreak of extended-spectrum β-lactamase producing Serratia marcescens in an intensive care unit
FEMS Immunology & Medical Microbiology, 1994
Serratia marcescens has recently been identified as an important etiological agent in nosocomial infections, and is considered to be an opportunistic pathogen agent in immunosuppressed patients undergoing long periods of intensive care. Research carried out in 1991 and 1992 showed that it was of epidemiological relevance in only 1-2% of clinical isolates at the Ospedale di Circolo, Varese, Italy. However, between 7 February and 11 October 1993, the incidence of cases attributable to S. marcescens had increased to 5%; 157 strains of Serratia marcescens were isolated from clinical specimens of 43 patients admitted to an intensive care unit; these strains, characterized by epidemic spread, showed the same pattern of multiresistance to antibiotics including monobactams and oxyimino-cephalosporins. During the same period 23 isolates were also recovered from 18 patients admitted to wards other than the intensive care unit; these strains, characterized by a wide range of antibiotic susceptibility, were also sensitive to/3-1actam antibiotics with the exception of first generation cephalosporins. The production of extended-spectrum /3-1actamases (ES/3Ls) and their genetic determinism were studied. All the epidemic strains of S. marcescens resistant to ceftazidime, cefotaxime, ceftriaxone and aztreonam produced three different/3-1actamases with pI 5.4, 5.5 and 8.4 respectively. In contrast, non-epidemic strains produced only a /3-1actamase with pl 8.4. The /3-1actamase with pl 5.5 was plasmid-mediated, hydrolizing ceftazidime and aztreonam, showing it to be an ES/3L; while the/3-1actamase with pl 5.4, although plasmid-mediated, did not hydrolize monobactams or oxyimino-cephalosporins. The /3-1actamase with pl 8.4 was found to be an inducible chromosomal enzyme capable of hydrolizing cefotaxime and ceftriaxone. Electrophoresis of the plasmid DNA indicated the presence of a similar plasmid of approximate size 54 kb in the resistant epidemic strains; this was found to be conjugative and mediating resistance also to aminoglycosides. Our data indicate that the plasmid-mediated production of ES/3Ls may contribute to the epidemic spread of Serratia marcescens in high-risk wards.
Journal of Hospital Infection, 2004
We investigated an outbreak of Serratia marcescens in the adult intensive care unit of the University Hospital of Napoli. The outbreak involved 13 cases of infection by S. marcescens over a nine-month period and was caused by a single pulsed-field gel electrophoresis clone. The epidemic strain was multiply antibiotic resistant, producing an inducible Amp C-type betalactamase enzyme and carrying the trimethoprim-resistance gene and the adenyltransferase gene, which confers resistance to streptomycin and spectinomycin, within a class 1 integron. Antimicrobial therapy with betalactams was associated with S. marcescens acquisition in the intensive care unit.
Microbial Drug Resistance, 2015
Serratia marcescens is one of the most important pathogens responsible for nosocomial infections worldwide. Here, we have investigated the molecular support of antibiotic resistance and genetic relationships in a series of 54 S. marcescens clinical isolates collected from Eastern Algeria between December 2011 and July 2013. The 54 isolates were identified by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS). Antibiotic susceptibility testing was performed by disc diffusion and E-test methods. Antibiotic resistance genes were detected by polymerase chain reaction (PCR). The genetic transfer of antibiotic resistance was performed by conjugation using azide-resistant Escherichia coli J53 as the recipient strain, and plasmid analysis was done by PCR-based replicon typing. The relatedness of our isolates was determined by phylogenetic analysis based on partial sequences of four protein-encoding genes (gyrB, rpoB, infB, and atpD) and then compared to MALDI-TOF MS clustering. Thirty-five out of 54 isolates yielded an extended-spectrum b-lactamase (ESBL) phenotype and carried bla CTX-M-15 (n = 32), bla TEM-1 (n = 26), bla TEM-71 (n = 1), bla SHV-1a (n = 1), and bla PER-2 (n = 12). Among these isolates, we identified a cluster of 15 isolates from a urology unit that coharbored ESBL and the 16S rRNA methyltransferase armA. Conjugation was successful for five selected strains, demonstrating the transferability of a conjugative plasmid of incompatibility group incL/M type. Phylogenetic analysis along with MALDI-TOF clustering likely suggested an outbreak of such isolates in the urology unit. In this study, we report for the first time the co-occurrence of armA methyltransferase with ESBL in S. marcescens clinical isolates in Eastern Algeria.
International Archives of Public Health and Community Medicine, 2021
This study investigated the antibiogram profile of Serratia marcescens among hospitalized individuals, hospital environments and halls of residence of Obafemi Awolowo University in Ile Ife, Osun state with a view to provide key information on resistance factors that are of therapeutic importance among the understudied pathogen. Two hundred and twenty samples from clinical and nonclinical sources were collected with ethical clearance approval (ERC/2018/09/02) from the hospital advisory committee. They were cultured on sorbitol Mcconkey agar infused with 200 U/ml of colistin for the selective isolation of Serratia species. Non-duplicate colony was picked from each cultures and characterized biochemically with the use of microbat 24E kit to identify various species isolated. The susceptibility profiles of the isolates against selected antibiotics were studied by the Kirby-baeur disc-diffusion technique. Results were interpreted according to CLSI (2019). Thirteen (61.9%) Serratia marces...