The Influence of Nasturtium officinale R. Br. Agar and Agitated Microshoot Culture Media on Glucosinolate and Phenolic Acid Production, and Antioxidant Activity (original) (raw)
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Transactions on Science and Technology, 2018
Watercress (Nasturtium officinale), a green vegetable belongs to the Brassicaceae, contains considerable amount of vitamins, minerals and secondary metabolites such as glucosinolates (GS). Watercress contains phenyl ethyl glucosinolate (gluconasturtiin), a precursor of phenyl ethyl isothiocyanate, which is widely reported to restrain the growth of cancer cells. The content of secondary metabolites and other compounds in plants is affected by different growth conditions such as pH, temperature, light intensity and nutrient supply. Thus, the aim of the current study is to evaluate the concentration of gluconasturtiin (and other GS) from in vitro grown watercress under non-elicited and elicited plant culture with wild plant of N. officinale. The samples were collected from watercress growing wild in a spring in Kundasang area, Ranau Sabah and subjected to sterilization to establish N. officinale in vitro culture under laboratory condition. The sterilization was done by using Chlorox® s...
Plant Cell Tissue and Organ Culture, 2021
Cultures of Nasturtium officinale were cultivated in vitro under illumination with different wavelengths of light-emitting diode (LED) light (white LED light-WLED, blue light-B, red light-R, 70% red and 30% blue light-RB, 50% green, 35% red and 15% blue light-RBG, 50% yellow, 35% red and 15% blue light-RBY, 50% far red, 35% red and 15% blue light-RBfR, 50% UV, 35% red and 15% blue light-RBUV, darkness-D), and under white fluorescent light (WF)-control conditions. The study investigated the influence of the applied lighting conditions on biomass growth and the production of glucosinolates, phenolic compounds, as well as photosynthetic pigments, and soluble sugars. The study showed a significant beneficial effect of the RBG light on biomass growth (Gi = 11.81 after 20 days) and the production of glucosinolates. The total glucosinolate content under these conditions increased 5.8 and 1.4 times in comparison with the WF light and D condition, respectively, reaching 237.92 mg 100 g -1 DW. The production of phenolic compounds, sugars, and photosynthetic pigments was comparable to the production under the control conditions. The antioxidant potential of extracts from the cultivated biomass was assessed by the CUPRAC, DPPH, and FRAP assays. Extracts obtained from the biomass of cultures grown under the RBG light had an antioxidant potential similar to that of the control cultures. This is the first report providing evidence of the stimulating effect of light quality on the biomass yield and production of glucosinolates by N. officinale microshoot cultures in vitro. The studies of different LED light quality on Nasturtium officinale microshoot cultures were proven that combination of 50% green, 35% red and 15% blue lights stimulated the production of glucosinolates, phenolic compounds, photosynthetic pigments, and influenced on the antioxidant potential of biomass extracts.
Phenolic profile and antioxidant potential of wild watercress (Nasturtium officinale L.)
SpringerPlus, 2015
Phenolic profile, antioxidant potential and pigment contents of wild watercress (Nasturtium officinale L.) were studied to assess the potential for future studies and its applications in neutraceuticals and bioactive functional ingredients. Different extracts of watercress (roots, stem and leaves) were analysed for pigment composition, total phenolic contents, and radical scavenging activity. The phenolic profile of the leaves and roots was studied using reversed phase HPLC-DAD. Results showed that total phenolic compounds in all samples were higher in the methanolic extracts than its corresponding aqueous extracts. The RSA of methanolic extracts was higher than aqueous extracts. Fourteen phenolic compounds were identified in the leaves, where coumaric acid and its derivatives, caftaric acid and quercetin derivatives were present in higher amounts. In roots, a total of 20 compounds was tentatively identified, with coumaric acid and its derivatives, sinapic acid, caftaric acid and quercetin derivatives were the major phenolic compounds. In conclusion, watercress has significant antioxidant activity and contains important phenolic compounds, which could be of potential biological interest.
Adaptation of the in vitro Culture of Origanum majorana L. For Production of Phenolic Acids
Origanum majorana L. (Lamiaceae) has a potent antioxidant activity due to its phenolic metabolites. Improving the accumulation level of the phenolic contents using plant tissue culture technique is outstanding aim. Seedlings were cultured on Murashige and Skoog (MS) medium with 6-benzyl amino purine (BAP) for shoot micro-propagation. The multiplied shoots were rooted on MS medium with 0.1 mg/L indole acetic acid (IAA) to obtain axenic plantlets. Root tips were sub-cultured in MS liquid medium with naphthalene acetic acid (NAA) and indole butyric acid (IBA) to initiate adventitious root suspension culture. The highest biomass obtained from the experiment with NAA; 0.5 mg/L (34.4 g, fresh weight). Callus grown on media with sucrose 3%, had the heaviest fresh and dry weight but the highest phenolic acids [caffeic, rosmarinic and chlorogenic acids] accumulation was with 60 g/L sucrose (HPLC analysis). Quantification of phenolic acids content in shoots and roots of field cultivated plant, in-vitro shoots culture (BAP, 1mg/L), in-vitro roots culture (IAA, 0.1 mg/L) and four treatments of adventitious root culture (0.1 and 0.5 NAA, 0.1 and 0.5 IBA mg/L), revealed that the sum of phenolic acids content was higher in in vitro shoot and root cultures (4.27 and 3.1 mg/g, respectively), while the adventitious root culture grown on media supplemented with 0.5 mg/L NAA, resulted in the highest sum of the target phenolic acids (45.01 mg/g). Elicitation with 200 µM methyl jasmonate (MJ) gave the highest callus biomass and the highest accumulation level of the phenolic acids, 105.82 fold of the field shoots contents.
Food Chemistry, 2016
The impact of conventional cooking and processing methods on total phenols, antioxidant activity, carotenoids and glucosinolates of watercress was evaluated. Boiling significantly decreases phenolic content, antioxidant activity and recoverable glucosinolates, however it increases the carotenoid concentrations of watercress as compared to the raw vegetable. Cooking by microwaving and steaming maintains the majority of phytochemicals in comparison to the fresh material, suggesting that they should be used as the preferred methods of watercress preparation. Boiling of watercress should be avoided to ensure maximum ingestion of watercress-derived beneficial phytochemicals.
Mini-Review on Plant In vitro Culture to Obtain Natural Chemicals
In Vitro Cellular and Developmental Biology-Plant, 2025
Natural plant metabolites, also known as secondary metabolites, are crucial for the survival and adaptation of plants in their environment. For modern society, these metabolites can be produced under uncontrolled or controlled environments. Alkaloids, flavonoids, terpenoids, phenols, lignans, saponins, coumarins, and glucosinolates are among the most important natural products from plants. Proteases from pineapple cultures in temporary immersion bioreactors have been obtained. . Additionally, the effects of sodium chloride, mannitol, and sodium azide on pineapple shoots propagated in temporary immersion bioreactors were recorded, with levels of chlorophylls, carotenoids, aldehydes, and phenolics measured. This short review summarizes the main results obtained.
2016
Asparagus officinalis as a valuable medicinal plant has a low multiplication rate using the conventional methods. This study was carried out to establish an efficient in vitro propagation protocol and also to compare some biological activities of in vivo and in vitro grown Asparagus. The nodal explants were cultured on MS medium supplemented with different concentrations of 6-benzylaminopurine (BAP) and 1-Naphthaleneacetic acid (NAA) or kinetin (Kn) and Indolebutyric acid (IBA), under light and dark conditions. After 6 weeks of culture, the highest percentage (100%) of callus formation was found in 17 of treatments under dark condition and 3 treatments under light condition. Also between the two groups of hormones, Kn +IBA showed better results in promoting callus formation. The highest average number of shoots (4.25) of size 4 mm or more per explant, formed under dark condition using 1.5 mg/L BAP mixed with 0.05 mg/L NAA. Rooting was best induced in shoots excised from shoot cultur...
Molecules, 2014
Ammonium nitrate (NH 4 NO 3 ), an important nitrogen source (34% N), has been used as an elicitor to stimulate plant growth and development as well as induce secondary metabolites under controlled conditions. In the present paper, we investigated the enhancement of cell biomass, total phenolics, resveratrol levels, and antioxidant activity of Vitis vinifera cv. Pok Dum by nitrogen enrichment (MS medium supplemented with NH 4 NO 3 at 0, 500, 1,000, 5,000 and 10,000 mg/L). The highest accumulations of biomass, phenolics and resveratrol contents were observed at 8.8-fold (86.6 g DW/L), 15.9-fold (71.91 mg GAE/g DW) and 5.6-fold (277.89 µg/g DW) by the 14th day, in the medium supplemented with 500 mg/L NH 4 NO 3 . Moreover, the antioxidant activities of cultured grape cells estimated by the DPPH • and ABTS •+ assay were positively correlated with phenolics and resveratrol, and the maximum activity was also observed in cultured cells with 500 mg/L NH 4 NO 3 at 176.11 and 267.79 mmol TE/100 g DW, respectively.
Artificial Cells, Nanomedicine, and Biotechnology
Prunella vulgaris L. is one of the therapeutic herbs containing various polyphenolics, which is used for multiple medicinal purposes. In this study, plant growth regulators (PGRs)-induced calli cultures from seed-derived leaf explants were exploited for the production of stress enzymes and polyphenolics. A growth curve was plotted for each PGR for 49 days period, which showed a distinct lag, log and decline phases. Here, the combination of naphthalene acetic acid (NAA) and 6-benzyleadenine (BA; 0.5 and 2.0 mg l À1 ) produced maximum fresh (6.32 FW-g/100 ml) and dry biomass (0.75 DW-g/100 ml) in contrast to control. The maximum synthesis of SOD (0.0154 FW-nM/min/mg) was detected on media comprising mixture of NAA and BA (1.5 mg l À1 ), while POD enzyme (0.366 FW-nM/min/mg) was higher at 0.5 mg l À1 NAA and 2, 4-dichlorophenoxy acetic acid. Further, NAA and BA (1.5 and 2.0 mg l À1 ) boosted up the synthesis of phenolics (18.83 GAE-mg/g-DW) and flavonoids content (18.05 RE-mg/g-DW) than control. Moreover, NAA of 1.0 and 2.0 mg l À1 were found supportive for maximum antioxidant activity (87.4%) and total protein (716 mg BSAE/mg-DW). This study will contribute in the development of cell culture in fermenter and synthesis of antioxidant secondary metabolites for commercial uses.