Aryl hydrocarbon (Ah) receptor DNA-binding activity. Sequence specificity and Zn2+ requirement (original) (raw)

Aryl hydrocarbon (Ah) receptor demonstrates sequence-specific DNA binding requiring Zn2+ and possibly other divalent metal ions. Analysis of synthetic oligonucleotides reveals a consensus sequence for DNA recognition. The receptor's binding activity is inhibited by metal-chelating agents, and restoration of activity depends on the presence of Zn2+. Findings align with the hypothesis that the Ah receptor is part of the steroid and thyroid hormone receptor superfamily, necessitating further investigation through cloning and gene transfer to confirm its membership.