Utility of ITS1 as a molecular tool for reliable identification of Henosepilachna pusillanima and H. septima (Coleoptera: Coccinellidae) (original) (raw)
Related papers
Annals of the Entomological Society of America, 1996
The Rice Weevil, Sitophilus oryzae (L.), and the maize weevil, S. zeamais Motschulsky, are sibling species of grain weevils that are usually distinguished by grain preferences and subtle differences in morphology. Previous findings of successful laboratory hybridization, genetic similarity of allozymes and chromosomes, and identity of aggregation pheromones raised questions about the validity of S. oryzae and S. zeamais as reproductively isolated biological species. We used molecular techniques to test the hypothesis that individuals assigned as S. oryzae or S. zeamais by morphological criteria represent members of 2 distinct gene pools, and hence are reproductively isolated species. Weevils from 18 different localities, which were collected from Africa, Australia, Asia, the south Pacific, and North America, were studied. All individuals were scored for the presence or absence of morphological characters that have been used historically for species determination. In most cases, determinations for the same individual were not consistent, depending on the morphological character used. The polymerase chain reaction (PCR) was used on the same specimens to analyze randomly amplified polymorphic DNA (RAPD-PCR) markers and to amplify selectively regions of mitochondrial DNA for analysis of restriction site polymorphisms with restriction endonucleases (RFLP-PCR). Both methods yielded markers that were consistently associated with either presence or absence of specific genitalic characters in both males and females. This correlation of molecular markers with genitalic morphotypes was consistent in all specimens studied, whether collected sympatrically from the same farms or from widely separated geographic populations, and supports a model of 2 reproductively isolated species. Other morphological characters involving pronotal punctures proved unreliable as correlates with genetic markers and are not useful for species diagnosis.
TJPRC, 2013
Results revealed that a single female of Epilachna vigintioctopunctata laid 211 to 328 eggs during life span. Eggs were yellowish elongated and cigar shaped often in a group of 5 to 45. Eggs were hatched into grubs within 5 to 11 days. Newly hatched 1st instar was yellowish in color and had six rows of long branched spines. The full frown 4th instar grub spent 1 to 3 days in pre-pupal stage. The pupal period lasted for 3 to 5 days with an average of 4.4 ± 0.89 days. The newly emerged adult was shiny yellow in color later changed to copper brown color mottled with black spots. Body was spherical with convex ventral surface and flat dorsal surface and gives appearance of ‘D’ when viewed from side. The average longevity of male and female was 51.4 and 64.8 days respectively. The pre-oviposition, oviposition and post-oviposition period were 21.4, 26.6 and 21.6 respectively. However present investigation clears that it is a pest of cucurbitaceous hosts also and is a serious pest of Momordica charanti. It causes defoliation and stunted growth and finally reduces yield of it.
Deutsche Entomologische Zeitschrift, 2019
Leaf-and fruit-feeding chrysomelids (Coleoptera) on bananas and plantains (Musaceae, Zingiberales) cause major losses to banana growers in the northern and northeastern regions of India, Bangladesh, and other parts of Southeast Asia. The species composition of these beetles has not been studied so far in India and wrong names or wrong name combinations in the literature have caused confusion. Most particularly, the Central and South American species of Colaspis hypochlora Lefèvre (Chrysomelidae, Eumolpinae) has been erroneously reported as occurring in India and Bangladesh, and this name has been used for the Indian species. Based on extensive surveys for leaf-and fruit-feeding chrysomelids in the northern and northeastern regions of India from 2015 to 2019, three species of banana-feeding chrysomelids, namely, Basilepta subcostata (Jacoby) (Eumolpinae), Bhamoina varipes (Jacoby), and a new species, Sphaeroderma cruenta sp. nov. (Galerucinae, Alticini), are documented. Of these, the latter two are recorded as pests of banana in India for the first time. An illustrated diagnostic account of these three species is given to facilitate their identification by economic entomologists. COI sequences of populations of B. subcostata from Assam and Uttar Pradesh showed 98-100% homology, indicating that these populations are conspecific and that COI sequences can be used for rapid species determination. Brief notes on the biology and available management options for these pests are also given.
The Journal of Basic and Applied Zoology
Background: More than 350 K described species, Coleoptera (beetles) represent the most diverse order from Class Insecta in the entire animal kingdom. However, their phylogeny is highly controversial due to their morphological crypticness and multiple markers used previously for sequence homology. Although many studies suggest that their diversity currently relies majorly on morphological analysis, nevertheless DNA barcodes may provide a functional, standardized tool for their unique identification. In the present report, a fragment of the mitochondrial cytochrome c oxidase I (COI) gene has been proposed as standard DNA barcoding marker for the identification of organisms. Result: To evaluate this hypothesis, a random sampling was conducted in and around Vadodara, Gujarat, where 2690 individual of 65 species belonging to 16 families were reported from different sites, and diversity indices were employed to unravel the species composition of that habitat. Further, 12 beetles from dominant families were selected for sequence analysis using various bioinformatics tools and were compared with the rest of the beetles to obtain a more robust phylogeny which is not reported earlier in previous studies. Conclusion: Hence, the present study suggests that Scarabaeidae tends to be more diverse in and nearby of Vadodara compared to all other families of Coleoptera and while in contrast Chrysomelidae showed maximum diversity of pest species. DNA barcoding and nucleotide analysis resolves the phylogeny of controversial taxa; (Adephaga(Gyrinidae+ (Dytiscidae++ Carabidae)) (Polyphaga(Histeridae+Buprestidae+Lampyridae+Elateridae+(Scarabaeidae+(Coccinelidae+ Apionidae+Curculionidae+ (Meloidae+Tenebrionidae+(Cerambycidae+Chrysomelidae)))))).
Taxonomic changes within the tribe Hyperini (Coleoptera: Curculionidae)
2008
The taxonomic status of three genera (Donus Jekel, 1865, Brachypera Capiomont, 1868 stat. restit., and Hypera Germar, 1817 in the tribe Hyperini is clarifi ed, including the identity of the type species of each genus, and problematic groups in each genus are commented. Neoglanis , is recognized as a junior synonym of Donus, because its type species, Phytonomus velutinus Boheman, 1842, is congeneric with Rhynchaenus philanthus Olivier, 1807, the type species of Donus. Subsequently, Brachypera is resurrected from synonymy and used to accomodate species previously assigned to Donus and not congeneric with Rhynchaenus philanthus; Phytonomus crinitus Boheman, 1834, is designated as the type species of Brachypera. Pseudhypera Capiomont, 1868, is a new junior synonym of Brachypera; Hypera reichei Capiomont, 1868, is designated as the type species of Pseudhypera. Antidonus Bedel, 1886, is transferred to Brachypera and included as a subgenus. A list of species belonging to the genera Donus and Brachypera is given, including 146 new combinations. The nomenclatoric situation of Phytonomus Schoenherr, 1823, previously recognized as an unnecessary replacement name for Hypera Germar, 1817, is reviewed. Capiomont, 1868; Dapalinus Capiomont, 1868; Boreohypera Korotyaev, 1999; Kippenbergia Alonso-Zarazaga 2005; Hypera s. str.) Hypera Germar, 1817 = Donus Dejean, 1821 (nomen nudum) = Ixus Dejean, 1821 (nomen nudum) = Glanis Dejean, 1821 (not Rafi nesque, 1818) (nomen nudum) = Phytonomus Schoenherr, 1823 (unjustifi ed replacement name) = Pytonomus: REDTENBACHER (1845) (incorrect subsequent spelling) = Phytonomidius Capiomont, 1868 = Dapalinus Capiomont, 1868 (valid subgenus) = Eririnomorphus Capiomont, 1868 (valid subgenus) = Erirhinomorphus: CAPIOMONT (1868b) (incorrect original spelling of Eririnomorphus rejected by NEAVE (1939)) = Erirrhinomorphus: WEISE (1891) (incorrect subsequent spelling of Eririnomorphus) = Tigrinellus Capiomont, 1868 (valid subgenus) = Boreohypera Korotyaev, 1999 (valid subgenus) = Kippenbergia Alonso-Zarazaga, 2005 (valid subgenus)
International Journal of Current Research in Biosciences and Plant Biology, 2016
This paper deals with a total of 44 species of Coleopterans (Insecta), under 35 genera, belonging to 9 families. These insect species are associated with 7 spp. of cereal crops (of 2 families), 10 spp. of vegetable crops (5 families) and 35 spp. of medicinal plants (14 families), occurring in diverse habitats in vast localities of Jammu, Kashmir and Ladakh regions of J & K State. The Coleopteran species, infesting cereal crops, vegetable crops and medicinal plants accounts for 25%, 36.36% and 50 % respectively of the total Coleopteran-fauna associated with crops and medicinal plants studied. The highest number of species of Coleopterans i.e. 14, pertaining to the family Curculionidae, is associated with host crops (cereals, vegetables) and medicinal plant species. This is followed by family Chrysomelidae and Scarabaeidae, with 11 spp. and 9 spp. respectively. Rest of the Coleopteran families show number of species either 2 spp. or 1 sp., associated with the vegetable crops and medicinal plant species. The family Solanaceae, including vegetable crops and medicinal plants, is affected by highest number of Coleopteran species, 13 (29.54 %). This is followed by cereal crop families, Fabaceae and Poaceae, affected by 6 spp. (13.63 %) each of Coleopteran. An updated systematic checklist of Coleopteran-fauna associated with host cereal and vegetable crops, and medicinal plant species has been given. Apart from this, a Catalogue on Host species-Coleopteran species complex, has been added.
Polymorphism of Cheilomenes sexmaculata (Fabricius) (Coleoptera: Coccinellidae) in Haryana, India
Journal of entomology and zoology studies, 2016
The study of polymorphism related to Cheilomenes sexmaculata (Fabricius) (Coleoptera: Coccinellidae) was conducted during the year 2015-16 in Haryana, India. The family Coccinellidae is known for the ladybird beetle species having different morphs showing variations of elytral colour patterns within and between the species. A large number of specimens were collected by sweep nets or handpicking from the agricultural as well as wild areas of the Haryana and six different morphs of this species were identified, comprising of three melanic and three non-melanic forms. The different morphs of the species were identified on the basis of the external characters and the shape of male genitalia. The pattern of the pronotum was similar while the pattern of the elytra was quite variable in all the six types of morphs. Thus the present study documents variable elytral colour polymorphism within C. sexmaculata in
A molecular phylogenetic study of Cucujidae s.l. (Coleoptera: Cucujoidea)
Systematic Entomology, 2015
Of all the superfamilies within the megadiverse order Coleoptera (Insecta), Cucujoidea (Cucujiformia) is arguably the most problematic taxonomically. The families comprising Cucujidae s.l. (Silvanidae, Laemophloeidae, Passandridae and Cucujidae s.s. represent a large portion of cucujoid diversity. Herein we present the results of a rigorous molecular phylogenetic analysis of Cucujidae s.l. using maximum-likelihood and Bayesian analyses of seven genes. Representatives of over half of the families of Cucujoidea (excluding the cerylonid series), as well as a broad sampling of Silvanidae and Laemophloeidae, were analysed. The monophyly of Cucujidae s.l. is rejected but a subgrouping of taxa that may form the core of a natural cucujoid lineage is recovered. This clade consists of two large monophyletic groups including several families each. Relationships among these smaller cucujoid groups are discussed, including several novel phylogenetic hypotheses, whereas morphological characters considered significant for classification in Cucujidae s.l. are evaluated in light of these phylogenetic hypotheses. Silvaninae, Telephanini, Brontini and Brontinae are recovered as monophyletic in the Bayesian analysis, but the former two are recovered as paraphyletic in the maximum-likelihood analysis. Our results support the placement of Psammoecus Latreille within Telephanini and also recover a paraphyletic Telephanus Erichson. Silvaninae is divided into three lineages, each representing a potential tribal lineage. Laemophloeidae is rendered paraphyletic in all analyses by Propalticidae and the latter is herein formally transferred to Laemophloeidae stat.n. Several suprageneric laemophloeid clades are recovered and discussed as potential higher-level groups. Laemophloeus Dejean is not recovered as monophyletic.
2011
Morphological and molecular analyses of Ips bark beetles collected in the eastern and northwestern Himalayan area resulted in the diagnosis of three species-Ips stebbingi, I. longifolia and I. schmutzenhoferi. These three species can be distinguished by morphological characters, including the frontal setae, the body size and the shape and position of the spines on the elytral declivity. DNA sequences of the mitochondrial Cytochrome Oxidase I gene were analyzed to detect haplotype variation within each of the species. A phylogenetic analysis was performed on our data plus data from Gen-Bank. Considerable amount of intra-specific sequence divergence was found in I. longifolia, ranging between 0.2%−2.5%. Intra-specific sequence divergence in I. stebbingi ranged from 0.2%−1.1% and in I. schmutzenhoferi it varied by 0.2%. Interspecific sequence divergence was high among the three species: 7.4−8.6% between I. longifolia and I. stebbingi, 11.9−12.2% between I. stebbingi and I. schmutzenhoferi, and 11.5−12.6% between I. longifolia and I. schmutzenhoferi. Phylogenetic analyses revealed Himalayan Ips species to be monophyletic and unrelated to other Asian species. Ips stebbingi and I. longifolia were sister taxa and I. schmutzenhoferi was distinguished from I. stebbingi.