Bioremediation Treatment of Polyaromatic Hydrocarbons for Environmental Sustainability (original) (raw)
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Journal of Experimental Biology and Agricultural Sciences
The term "hydrocarbon" is self-explanatory and refers to solely carbon and hydrogen compounds. Hydrocarbons play an important role in our everyday lives. Hydrocarbons, particularly polycyclic aromatic hydrocarbons, harm biota. The relatively fast introduction of xenobiotic compounds, as well as the enormous movement of natural materials to various environmental compartments, can often overwhelm the self-cleaning capabilities of the recipient ecosystem, resulting in pollution and accumulation of hazardous or even lethal levels. Bacteria capable of hydrocarbon degradation are frequently used in the bioremediation of fuel oil-contaminated sites. Presently, multiple sophisticated methodologies, transcriptomics, proteomics and are effectively utilized for the depiction of hydrocarbons degrading microorganisms. These expertises are highly developed, and its integration with bioinformatics tools makes it even more efficient. Though health science and biological science are the ma...
One of the main methods for removing petrol and other hydrocarbon chemical pollutants from the environment is through the use of microorganisms that are already present in contaminated soils. Exploring the frequency of isolated bacteria from oil and measure the ability of modified bacteria to hydrocarbon degradation. Twelve bacterial isolates from different oil factories. The samples were routine culture methods and test utilized to detect and as well they further confirmed using Vitek 2. Furthermore, hydrocarbon degradation test done by depended on the color change in the nutrient medium as an indicator of. Three isolates were obtained from the screening these isolates, namely Pseudomonas aeruginosa, Escherichia coli, and Sphingomonas paucimobilis. The E. coli and Ps.aeruginosa were high frequencies isolated from oil felid (60%, 30%, respectively). Furthermore, based on the Biodegradation Productivity, results after twenty-eight days the top-performing bacterial isolate for phenanthrene degradation were (Sphingomonas paucimobilis) wild type (43.6%). In terms of naphthalene breakdown, the highest degradation effectiveness was observed in (E. coli) type with a ratio of (97.7%). As for acenaphthene, (E. coli) wild type exhibited excellent degradation efficiency (88.6%).
International Journal of Environmental Science and Technology, 2018
Oil pollution is a major global environmental concern. Bioremediation is considered as a suitable approach for remediation of oil-contaminated environments. In this study, two crude oil-contaminated soils were collected to isolate and identify some native and active oil-degrading bacteria to be used in remediation of contaminated sites. Five isolates were selected according to "hole-plate diffusion method" and were grown in crude oil. They were cultured in a mineral salt medium in which crude oil was employed as the sole carbon source. Biochemical, morphological and genomic identifications demonstrated the bacteria species as Pseudomonas resinovorans, Plantibacter auratus, Bacillus subtilis, Staphylococcus pasteuri and Bacillus atrophaeus. These bacteria were able to degrade 86.0%, 61.3%, 81.1%, 55.0% and 76.2% of aliphatic compounds and 58.6%, 39.4%, 55.5%, 39.0% and 49.9% of aromatic hydrocarbons in a medium containing crude oil (1% v/v) over 21 days, respectively. The degradation rates of aromatic compounds from 14 to 21 days were higher than of aliphatic hydrocarbons. This rate was 28.4% by Bacillus subtilis, 30.9% by Bacillus atrophaeus, 27.2% by Staphylococcus pasteuri and 21.3% by Pseudomonas resinovorans. In Plantibacter auratus, this rate was 16.19% which is less than aliphatic hydrocarbons. To our knowledge, it seems this is the first time to report Pseudomonas resinovorans and Plantibacter auratus as crude oil degraders. Results of this study indicated that the isolated bacteria could have a high potential to be used in bioremediation of oil-contaminated environments.
An Approach for the Biodegradation of Polycyclic Aromatic Hydrocarbon
Journal of Experimental Biology and Agricultural Sciences, 2021
Environmental pollution not only alters the environment but also changes the growth rate of various flora and fauna. Due to the irresponsible disposal of waste materials, the environment is going to be more and more polluted. Discharge of hydrocarbons in the water bodies is contaminating the water sources. These hydrocarbons are affecting the living organism. The solution to this problem has been found too expensive with little effects. To overcome this problem, some biological methods are introduced, in biological method; microbial degradation of hydrocarbons is the most promising method. The proposed study aimed to isolate and identify hydrocarbon-degrading bacterial species from the Mathura refinery, Mathura U.P. India. These species were isolated from petroleum-contaminated refinery water and grew on nutrient agar media, identified according to their distinct morphological, and biochemical characteristics, with16s rRNA sequencing. Results of this study confirmed the presence of ...
Applied Microbiology: open access, 2015
Polycyclic aromatic hydrocarbons (PAHs) are common environmental pollutants and biodegradation using microorganisms is the preferred and major route of PAH removal from contaminated environments. This study investigated the bacterial degradation of petrol and diesel in liquid media that were isolated from oil contaminated soils by enrichment technique. The isolates could use petrol and diesel as their sole carbon and energy source in Bushnell Hass Mineral Salts (BHMS) medium at 2% (v/v) concentration. A total of eight isolates were selected and characterized by using a variety of phenotypic and morphologic properties. Two isolates each showed highest growth in petrol and diesel containing media during screening were selected and characterized using 16S RNA sequencing. Molecular identification of the isolates assigned them to Achromobacter sp. and Pseudomonas aeruginosa. The selected isolates degraded petrol and diesel up to 31.9% and 34.4% respectively. This study indicates that the contaminated soil samples contain a diverse population of PAH-degrading bacteria and the use of Achromobacter sp. and Pseduomonas aeruginosa has the potential for bioremediation of PAH contaminated sites.
Characterization of Hydrocarbon Degrading Bacteria Isolated From Hydrocarbon Contaminated Soil
Polycyclic aromatic hydrocarbons (PAHs) are of particular concern due to its toxic and carcinogenic properties. The fate of these PAHs and other associated compounds present in the environment is directly related to abiotic and biotic processes; including oxidation, bioaccumulation and degradation by microbes. This study was conducted for the isolation of bacteria with potential biodegradation abilities. Nineteen different bacterial isolates were identified from soils contaminated with PAHs with biodegradation capabilities of different hydrocarbon compounds (1 %) (Xylene, Benzene, Naphylamine, Diphenylamine) added to nutritional media (L. agar and MM 2 Agar). These bacterial isolates were found able to grow in the presence of different metals stress along with hydrocarbon at different temperature (25 to 45) and pH (5 to 9) conditions. Majority of the isolates were found belonging to Enterobacteriaceae family, while some of them were from Pseudomonas and Azotobacteriaceae. The bacterial isolates were capable of degrading a verity of different hydrocarbons with its ability to grow in different metals stress environment. Such bacteria can be successfully used for the bioremediation of industrial wastes.
Environmental Contamination: Petroleum Hydrocarbon and its Biodegradation
Agrobios Research: An Imprint of Agrobios (India), Jodhpur, 2021
Bioremediation of crude-oil contaminated soil is a successful process to a cleanup method for hydrocarbon contaminants from the ecosystem. In this study, the crude-oil degrading bacteria along with nutritional supplements and could revive crude-oil polluted soil very efficient in large-scale capacity. Today, one of the main ecological problems is hydrocarbon pollution consequential from the actions associated with the petrochemical industry. Accidental releases of petroleum products are of particular concern in the surrounding. Chemical and mechanical methods commonly used to remove hydrocarbons from contaminated sites have partial efficacy and can be very expensive. Bioremediation shows potential technology for the treatment of these contaminated sites since it is cost-effective and will lead to total mineralization into the water, inorganic substances, carbon dioxide and cellprotein or transformation of complex to simple form organic compounds pollutants. This review presents a comprehensive account on the petroleum hydrocarbon degradation through microorganisms under variable types of the ecosystems. It focuses on the degradation of PHs, emphasized the applicable biological and physicochemical aspects and the enzymatic methods influencing the processes.
Journal of Petroleum & Environmental Biotechnology, 2010
Five bacterial strains from genera Pseudomonas, Rhodococcus, Micrococcus and Bacillus isolated from petroleum-contaminated soils were selected for their capacity to grow in the presence of petroleum and some aromatic hydrocarbons. Their growth rates and biodegradation ability were investigated in mineral basic media supplemented with light oil, crude oil, aniline plus catechol, aniline, toluene and naphthalene. The results revealed the extents to which these strains could degrade different aromatic hydrocarbon that are toxic in the environment. The plasmid profi les of isolated bacteria were also determined and in some strains, biodegradation ability proved to be plasmid related.