Water Fraction Of Sambiloto (Andrographis Paniculata Nees) Ethanol Extract Efficacy In Inducing The Number Of Macrophage, Neutrophil, And The Level Of TNF-α On Wistar Rats (original) (raw)

Background. The study of the effect of water fraction of Sambiloto (Andrographis paniculata Nees) ethanol extract on the number of macrophage, neutrophil, and levet of TNF-α in the body fluid of male Wistar rats after Staphylococcus aureus induction. Methods. Animals were divided into 3 groups, namely: negative control (NC), treatment (T), and positive control (PC) group. The NC, T and PC groups were administered by 0,9% NaCL, water fraction of Sambiloto ethanol extract of 40mg/200g BW dose, and ibuprofen of 7.2 mg/200g BW dose, respectively. The treatment was performed for 7 consecutive days. The number of macrophages, neutrophils, was identifed using light microscope whereas the TNF-α. level was identified using ELISA. Outcome Measured. The number of macrophage, neutrophil, and level of TNF-α in the body fluid of male Wistar rats after Staphylococcus aureus induction. Results. The number of macrophages in the KC, the T, and the PC groups were 2.33 ± 1,58; 3.80 ± 4.06; and 2.67 ± 1...