Spectrum of melanoma antigens on cultured human malignant melanoma cells as detected by monkey antibodies (original) (raw)
To characterize the antigen present on the surface of cultured human malignant melanoma, three monkey xeno geneic antisera were raised. After appropriate absorption with pooled human erythrocytes, peripheral blood leuko cytes, and liven homogenate, no blood group or HLA neac tivity was detectable. Analysis of melanoma antigenic spec ificity was performed by the mixed hemadsonption microas say on live monolayer cells in conjunction with quantitative microabsorption analysis. To eliminate reactivity against nonmelanoma lines, 2 of the 3 antisera required further absorption with cells of the KB oral carcinoma line. The absorbed antisera reacted with 9 of 10 long-term estab lished lines, 3 of 3 short-term cultures of human malignant melanoma, and i of 10 nonmelanoma epithelial and fibro blastic cell lines. Absorption experiments using a variety of cultured cells and fresh tissue homogenates of adult human melanoma and nonmelanoma sources further substantiated the results obtained with the direct tests. While each mela noma line showed a differing degree and pattern of neactiv ity, the antisera were most reactive against their respective immunizing lines as revealed by both the direct tests and absorption analysis. By quantitative absorption analysis, no evidence for individually specific melanoma antigens was obtained. The only positive absorption with nonmelanoma adult tissues was obtained with a retinoblastoma cell line, indicating the presence of antigens shared by tumors of common neunoectodermal origin. Extensive absorption with two xenogeneic malignant melanoma (munine and porcine) homogenates, normal human adult skin, and spleen tissues, or Bacillus Calmette-Guéninfailed to reduce the reactivity against melanoma-associated antigens. Fur then absorption with human fetal tissues of 8 to 20 weeks of gestation removed part but not all of the reactivity. These studies with xenogeneic monkey antisera provide evidence for the existence of common melanoma-associated anti gens as well as distinct but shared human fetal antigens on human melanoma cells. , This is Paper 8 of the series, â€oeCharacterization of Human Malignant MelanomaCell Lines.― The work was supportedby the Medical Research Council of Canada and the Ontario Cancer Treatment and Research Foun dation.
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