Biological activities of polyphenols-enriched propolis from Argentina arid regions (original) (raw)
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Antimicrobial activity, phenolic profile and role in the inflammation of propolis
Food and chemical toxicology: an international journal published for the British Industrial Biological Research Association
Nowadays a great amount of information regarding chemical and biological aspects of bee products is available in the literature, but few data on its therapeutic uses are found. The aim of this study was to evaluate the phenolic profile, the in vitro antimicrobial activity and effect in the hyaluronidase enzyme (widely related with the inflammation process) of propolis harvested in Portugal. The efficacy of three extracts (hydro-alcoholic, methanolic and aqueous) was also compared. It was chosen the hydro-alcoholic extract, because this was the most effective for extracting phenolic compounds. The antimicrobial activity was accessed in Gram-positive and Gram-negative bacteria and yeasts, isolated from different biological fluids and the results were then compared with the obtained for reference microorganisms. The propolis from Bragança was the one that possessed the highest polyphenols' content. The sample from Beja showed the less significant inhibition of the hyaluronidase enz...
Propolis from stingless bees Tetragonisca fiebrigi found in Brazil is used in folk medicine by their nutritional and therapeutic properties. However, there are no scientific records evidencing such properties. The present study was designed to investigate the chemical composition and the biological properties of propolis from T. fiebrigi. For this, the chemical composition of the ethanol extract of propolis (EEP) was determined by GC-MS and presented phenolic compounds, alcohol, and terpenes as its major class compounds. The antimicrobial activity was accessed in gram-positive and gram-negative bacteria and in fungi, isolated from different biological fluids and reference strains. The EEP was active against all microorganisms and showed antioxidant activity by scavenging free radicals, inhibiting hemolysis and lipid peroxidation in human erythrocytes incubated with an oxidizing agent. The anti-inflammatory potential of the EEP was confirmed by inhibition of the hyaluronidase enzyme. The cytotoxic activity was concentration-dependent against K562 cells, with a predominance of death by necrosis. Taken together, these results show that propolis from T. fiebrigi has important therapeutic activities, which suggest its potential application in the pharmaceutical industry, as well as in health foods, beverages, and nutritional supplements.
Phytochemical Analysis and Antioxidant Properties of Amber and Red Honeybee Propolis
Honeybee Propolis has been used in ethno medicine as an emollient in the treatment of measles, chicken pox, ringworm, and it was also reported that biological activities, such as antitumor, antioxidant, immunomodulatory action and anti-inflammatory has positive effect to propolis. The aim of the study is to determine the Phytochemical constituents and antioxidant properties. Preliminary phytochemical screening of the ethanol extract propolis of both samples revealed the presence of flavonoids, phenolic compounds, saponins, carbohydrate, tannins, steroids and reducing sugar. The antioxidant activity of the two samples were determined using stable 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical assay and was found to be all positive. The percentage scavenging activity of amber ethanol extract propolis is between the range of 47.07-53.84% which is the lowest and that of red ethanol extract propolis is between 47.52-63.58% which is the highest. The 50% inhibitory concentration (IC50) of red propolis was found to be 60µg/ml and 140µg/ml for amber propolis. The low value of IC50 in red propolis can be attributed to high composition of flavoniods and phenolic nucleus which make it to have significantly more antioxidant and free radical scavenger activities than the amber propolis.
International Food Research Journal
The present work examined the in vivo antispasmodic and nephroprotective potentials of methanolic extracts obtained from Algerian native propolis and bee pollen. The in vivo antispasmodic activity was assessed by the intraperitoneal injection of acetic acid (1%) which induced long-lasting visceral pain in mice. The renal damage was modelled by intraperitoneal injection of a cisplatin (CP; 10 mg/kg) followed by histopathological changes in kidneys. In addition, the beehive by-products were screened for their bioactive content and in vitro antioxidant activities. The propolis and bee pollen are rich sources of bioactive compounds. The propolis showed the highest antioxidant potencies as evaluated by β-carotene bleaching system (87.16 ± 3.69%), DPPH (176.05 ± 0.20 µg/mL), and FRAP (0.61 ± 0.002 μmol Fe(II)/g) assays. The antispasmodic test revealed that propolis extract (250 mg/kg) significantly inhibited the number of spasms (61.04 ± 3.92%) induced by acetic acid. Based on histopathol...
Studies of the Biological and Therapeutic Effects of Argentine Stingless Bee Propolis
Journal of Drug Delivery and Therapeutics
Meliponids are native bees of the Americas, characterized by having no sting (ANSA). Some live in the Yunga forests of northern Argentina, a place rich in a diversity of this type of bees of which at least thirty-three species may be found. Propolis is a resinous substance that bees collect from plants exudates. Chemical composition and functional properties vary according to the flora of the place where the hives are. They have been valued by humans for their biological properties for centuries. This study is aimed at investigating the antinociceptive, antioxidant and anti-biofilm activities of propolis from the stingless bees Tetragonisca fiebrigi Schwarz and Scaptotrigona jujuyensis Schrottky found in Estación Experimental Agropecuaria Famaillá of INTA, Tucumán, Argentina. Analgesic activity of the extracts was estimated against acetic acid induced writhing, tail immersion method and formalin test. Antioxidant capacity was evaluated using DPPH free radical scavenging and β-carote...
ANALYSIS OF PROPOLIS STINGLESS BEE BIOACTIVE COMPOUNDS FROM SEVERAL REGIONS IN INDONESIA
International Journal of Applied Pharmaceutics, 2024
Objective: Propolis is a bee product collected by honeybees from a resinous substance of various plant sources. Its antioxidant activities are different from various geographic origins. This study aimed to analyze bioactive compounds by lC-MS/MS, compare antioxidant activity total phenolic and flavonoid contents in stingless bee propolis samples from several regions in Indonesia. Methods: The propolis samples were taken from stingless bee hives of Tetragonula clypearis from Sumbawa, Tetragonula laeviceps from Magelang, Tetragonula biroi from Bogor, and Geniotrigona thoracica from South Kalimantan). Analysis of bioactive compounds was identified by lC-MS/MS. The quantification of the chemical compound determined its total phenolics and flavonoid (TPC and TFC) contents. The antioxidant activity was determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging assay.
Biological activity of bee propolis in health and disease
Asian Pacific journal of cancer prevention : APJCP
Propolis is a natural product derived from plant resins collected by honeybees. It is used by bees as glue, a general-purpose sealer, and as draught-extruder for beehives. Propolis has been used in folk medicine for centuries. It is known that propolis possesses anti-microbial, antioxidative, anti-ulcer and anti-tumor activities. Therefore, propolis has attracted much attention in recent years as a useful or potential substance used in medicine and cosmetics products. Furthermore, it is now extensively used in foods and beverages with the claim that it can maintain or improve human health. The chemical composition of propolis is quite complicated. More than 300 compounds such as polyphenols, phenolic aldehydes, sequiterpene quinines, coumarins, amino acids, steroids and inorganic compounds have been identified in propolis samples. The contents depend on the collecting location, time and plant source. Consequently, biological activities of propolis gathered from different phytogeogra...