In vitro acylation of the transferrin receptor (original) (raw)

In vitro fatty acylation of the transferrin receptor in isolated sheep reticulocyte plasma membranes was investigated, demonstrating that although less than 5% of the receptor was labeled, acylated protein could be detected through electrophoresis. The study highlights that acylation is ATP-dependent and shows that fatty acid and its acyl-CoA derivative have different efficacies as acyl donors. Additionally, the transference of the labeled receptor to vesicles suggests a mechanism of retention of acyl groups during vesiculation, which may inform future research on membrane protein modifications.