Bio-impedance detector for Staphylococcus aureus exposed to magnetic fields (original) (raw)
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Magnetic Field Effect on Growth and Antibiotic Susceptibility of Staphylococcus aureus
Journal of Al-Nahrain University Science, 2017
It is aimed to investigate the effect of exposure of different magnetic fields (400, 800.1200 and 1600 Gauss for 2 to 24 hours) on the growth rate and antibiotic sensitivity of Staphylococcus aureus. The experiments were done in Hawler Medical Research Centre Erbil, Iraq. The bacteria were isolated from medical case in Rizgary hospital and identified using API STAPH system. The antibiotic susceptibility of Staphylococcus aureus measured according to Kirby-Bauer disc diffusion technique. Results showed a significant decrease in the logarithm in the number of Staph. aureus (41.4 to 27 X 10 6) treated with high frequency magnetic field. Sensitivity of Staph.aureus to antibiotic increase during a short period (4-6 hours) and increase its resistance to same antibiotic at log term of exposure (18-24 hours). Some results of biochemical tests also showed positive effects of magnetic fields on the biochemical properties according to API STAPH results. The bacterial enzymes MAL (Maltose), LAC (Lactose), TRE (D-Trehalose), MAN (D-Mannitol), SAC (Sucrose) and NAG (N-acetyl-glucosamine) were affected by magnetic field at 24 hours of incubation. It is concluded that the cellular membrane of the microorganism had been affected by the magnetic field, also the response increased when the field intensity increased. So the magnetic field effects on bacteria are considered bactericidal, and hence, a change in the number of the cells per ml or the measured change in the membrane sensitivity to antibiotic demonstrated also the change in the internal structure of the cells.
Effect of Frequency Magnetic field on Gram Positive and Gram Negative Bacteria
2019
Objective It is necessary to evaluate the harmful or useful effects of electromagnetic waves on living organisms and determine the threshold of these radiations. In this research, the effect of magnetic fields on the growth of gram-positive ( Staphylococcus aureus ) and gram-negative ( Escherichia coli ) bacteria has been evaluated.Results In Gram-negative bacteria such as E. coli in both magnetic fields 1mT and 2mT at different frequencies, an additive effect was seen on the growth of bacteria. When the frequency increased the trend of increasing bacterial growth, slowed. In Gram-positive bacteria such as Staphylococcus, this effect was less. In 1mT magnetic field, the growth of bacteria was seen but the 2mT field was virtually ineffective and the differences between two groups at different frequencies were not significant. Also, significant changes didn't observe with increasing frequency. Study of bacterial growth in terms of frequency in both case and control groups showed a...
Electromagnetic biology and medicine, 2015
The impact of different types of extremely low-frequency electromagnetic fields (ELF-EMF) on the growth of Staphylococcus aureus and Escherichia coli O157:H7 was investigated. The cultures of bacteria in broth media were exposed to sinusoidal homogenous ELF-EMF with 2 and 4 mT magnetic intensities. Each intensity for each bacteria was combined with three different frequencies (20, 40 and 50 Hz), and four different exposure times (1, 2, 4 and 6 h). A cell suspension of each experiment was diluted for the appropriate range and inoculated to Mueller-Hinton Agar (MHA) plates after exposure to ELF-EMF. The number of colony forming units (CFU) of both strains was obtained after incubation at 37 °C for 24 h. Data were statistically evaluated by one-way analysis of variance (ANOVA), statistical significance was described at p < 0.05 and data were compared with their non-exposed controls. Magnetic intensity, frequency and exposure time of ELF-EMFs changed the characteristic responses for ...
The Effect of Static Magnetic Field on E. coli, S. aureus and B. subtilis Viability
Journal of Natural Sciences Research, 2015
This study investigated the effects of low static magnetic field on the growth of three bacterial strains ( Escherichia coli, Staphylococcus aureus and Bacillus subtilis ) that plays a versatile role in infecting wounded tissues. The viability of these bacteria was measured with and without different magnetic fields (30, 50, and 80) mT after 24 hours. Results illustrate that magnetic field decreased the growth rate of Escherichia coli and Staphylococcus aureus bacteria, while increased the growth rate of Bacillus subtilis after 24 hours of exposure. Keywords: Magnetic field, Bacillus subtilis , Escherichia coli , Staphylococcus aureus .
Growth Rate of Staphylococcus Aureus in weak magnetic field
2020
A comparative study of the growth of S.aureus in different types of weak magnetic field shows that the growth of the bacteria is initially suppressed after inoculation in a new medium and then increased in the same pattern with or without magnetic field. However, the rate of growth and death of bacteria depend on the variation in the magnetic field for comparable strength. Magnetic field effect on the ionic motion plays a key role in the growth rate. It is shown that the concentration of bacteria reduces initially and then it starts increasing after some time. Multiplication and death of bacterial cells is related to its interaction with the nutrients which is managed by the magnetic field direction, strength and variation at room temperature.
Frequency Electromagnetic field effects on Gram-Positive and Gram-Negative Bacteria
2019
Objective: The effects of electromagnetic waves on the growth of living organisms and the determination of the threshold of these radiations have remained elusive. Therefore, in this research, we have investigated the growth rate of gram-positive (Staphylococcus aureus) and gram-negative (Escherichia coli) bacteria that had been exposed to the different frequencies of electromagnetic fields. Results: The more frequency increased the slower bacteria grew; however, in gram-positive bacteria such as S. aureus, this effect was seen less. The effect of the 1mT electromagnetic field in the growth of S. aureus was significant between the two groups, nonetheless, in the 2mT electromagnetic field, the effect was not significant between the two groups at different frequencies. Noteworthy, no significant change was observed by increasing the frequency in S. aureus exposed bacteria in comparison to the control group. The study of bacterial growth in terms of frequency in both case and control g...
Use of electrical and magnetic fields in the control of bacterial biofilm
1997
Bacterial biofilm infections are less susceptible to antibiotic treatment than their planktonic counterparts. This has become a major health issue in many areas of a hospital and/or health care industry. The bioelectric effect was discovered by a team of Canadian scientists in 1994. The bioelectric effect is the treatment of the bacterial biofilm with antibiotic in the presence of an electrical current. This treatment has been found to produce a remarkable higher kill of the bacterial biofilm. This thesis replicated the original work done in Canada and from that base it goes into developing a dose response for the applied current in the presence and the absence of antibiotics. It shows that the effect is obtained with pure DC current and that the effect is not dependent on the direction of the current with respect to the nutrient flow. It also shows that the effect is only seen when the biofilm itself is treated with the DC current. We also have shown that an E-Field does not seem to have the same effect on the biofilm. Preliminary work was done using currents induced by AC fields and chemical manipulation of the nutrients. A mechanism for the bioelectric effect is proposed.
European Scientific Journal, 2014
In this work, the frequency of electric impulses that interfere with the bioelectric signals generated during staphylococcus aureus (S.aureus) cellular division is investigated. The experiment was expanded to in vivo study for the obtained data in which rats were infected with S.aureus and then whole body exposure were exposed to square electric pulses (SEP) that causes inhibition to the microbial cellular growth. Another group of animals was infected by previously inhibited bacteria with SEP then the histological and molecular structures of the liver were investigated for all the animal groups, Dielectric relaxation studies for the liver in the frequency range 42 KHz-5MHz was used to determined molecular structure changes. The results indicated a highly significant inhibition of cellular growth for S.aureus in addition to pronounced changes in the cellular morphology after the exposure of the microorganism to the resonance frequency of 0.8 Hz SEP for 120 minutes. From the histological and dielectric relaxation measurements and results it was indicated that the liver for animals infected by S.aureus and then exposed to SEP showed significant improvement in their health state as compared with infected and non exposed group. Moreover, the liver for the animals infected with previously treated bacteria with SEP showed highly significant decrease in cellular damage as S.aureus by 0.8 Hz SEP acts on the structure and biological activity of the bacteria and it is a promising methodology to control S.aureus activity in vivo and in vitro applications.