In silico study to breaking mystery of bioluminescence protein structure of bacterial luciferase and firefly luciferase (original) (raw)

2014, Bulletin of Environmental Science and Sustainable Management (e-ISSN 2716-5353)

Bioluminescent proteins such as luciferase or green fluorescent protein become an interesting protein to be studied particularly on its structure, mechanism of light emission in certain wavelength and also its contribution in several applications. This study was conducted to understand more on the luciferase enzymes from bacteria and firefly based on in silico studies of their protein sequences, protein surface and interactions with different substrates. Protein crystal structures of bacterial luciferase and firefly luciferase that been deposited on Protein Data Bank (PDB) were used as model proteins for this study. Computer analysis of luciferase proteins such as pI prediction, multiple sequence alignment and molecular docking with different luciferase substrates (Flavin mononucleotide, L-luciferin, D-luciferin, vargulin, coelectrazine, Dinoflagellate luciferin, Latia luciferin) by using iGEMDOCK docking software had been conducted. Final analysis showed that, the bacterial lucifer...

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