Phosphate binding to Escherichia coli alkaline phosphatase. Evidence for site homogeneity (original) (raw)

Phosphate binding to Escherichia coli alkaline phosphatase has been investigated, revealing evidence for homogeneous site binding. Contrary to previous reports of anticooperative binding, results suggest that observed anti-cooperativity may be an artifact of experimental methods. The study utilizes equilibrium dialysis to clarify the binding process, indicating that the two active sites of the enzyme bind inorganic phosphate uniformly, independent of temperature and ionic strength, challenging prior findings of site heterogeneity.