In vitro antioxidant, antimicrobial and cytotoxic activities of the various extracts of Ganoderma lucidum available in Bangladesh (original) (raw)
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2018
Ayurvedic treatment is one of the most ancient treatment systems against various diseases in Indian subcontinent and the western countries have also begun to take this alternative natural therapy as their major treatment system. The medicine of ayurvedic treatment came from various plants or plant parts. Ganoderma lucidum is one of fungus that has been extensively used as various therapeutic agents and contains approximately 400 different bioactive compounds which have been reported to have a number of pharmacological effects. But the biochemical composition of G. lucidium can be changed from its native origin. The biochemical composition, antioxidant activity, cytotoxic activity and antibacterial activity of the G. lucidium cultivated in Bangladesh were detected in this experiment. The phytochemical screening of aqueous extract of G. lucidium was determined using standard methods, the antioxidant activity was evaluated by DPPH free radical scavenging assay, the cytotoxic effect was...
Emirates Journal of Food and Agriculture, 2015
Ganoderma lucidum (Fr.) Krast, a basidiomycete belonging to the Ganodermataceae family is one of the most famous traditional Chinese medicinal herbs, used as a healthy food and in medicine. In Brazil, some people produced also in the same process and is indicated that is a nutriceutical product. The dry Ganoderma lucidum of three different states of Brazil were milled and extracted with hot water, water and ethanol, and ethanol only. The results obtained demonstrated that extracts with HotWater and Water-ethanolic presented more concentration of beta-glucan and proteins. Extract ethanolic presents higher concentration of free sugar and increased concentration of phenols when compared with others extracts but doesnt have protein. When make a mixture of water extract with alcohol, there is a higher concentration of beta-glucan with protein and phenol. When compared samples from three states, the one from Brasília presented more beta-glucan, phenols, proteins and free sugar in all extractions. The hydroethanolic extract showed tannins, flavonoids, terpens, steroid nucleus. It showed an anti-inflammatory and antimicrobial activity when tested with specific reaction for each group. It showed an antiinflammatory activity when tested by cotton pellets implantation in rats and antimicrobial activity in pour plate assay for Staphylococcus aureus and Candida albicans. It did not exhibit acute toxicology in acute model in mouse.
International journal of applied research, 2016
Medicinal mushrooms are widely used as traditional medicinal ingredients for the treatment of different diseases and related health problems. Ganoderma lucidum provides bioactive compounds that claim to possess antibacterial activity. The fruiting bodies of Ganoderma lucidum were collected from various places in Bilaspur district of Himachal Pradesh, India. The aim of this research is to know antibacterial activity of methanolic and acetone extracts of Ganoderma lucidum. Antibacterial activity of Ganoderma lucidum was done against four human pathogenic bacteria i.e. Yersinia pestis, Pseudomonas aeruginosa, Listeria monocytogenes and Bacillus cereus using agar well diffusion assay. The result showed that the extracts had antibacterial activity and methanolic extract had higher antibacterial activity than acetone extract.
International Journal of Food Sciences and Nutrition, 2014
A detailed comparative study on chemical and bioactive properties of wild and cultivated Ganoderma lucidum from Serbia (GS) and China (GCN) was performed. This species was chosen because of its worldwide use as medicinal mushroom. Higher amounts of sugars were found in GS, while higher amounts of organic acids were recorded in GCN. Unsaturated fatty acids predominated over saturated fatty acids. GCN revealed higher antioxidant activity, while GS exhibited inhibitory potential against human breast and cervical carcinoma cell lines. No cytotoxicity in non-tumour liver primary cell culture was observed for the different samples. Both samples possessed antibacterial and antifungal activities, in some cases even better than the standard antimicrobial drugs. This is the first study reporting a comparison of chemical compounds and bioactivity of G. lucidum samples from different origins.
Scientific Reports
Ganoderma lucidum methanolic extract (GLME) has attracted tremendous attention due to its exceptional antimicrobial and anticancer properties that can be delicately tuned by controlling the initial extraction's content and concentration. Herein, we detailed the characterization, antimicrobial, and cytotoxic performance of GLME as a potential multi-functional therapeutic agent. Accordingly, FTIR, XRD, FESEM, EDX, and HPLC analyses were employed to assess the samples, followed by disc diffusion and microdilution broth methods to test its antibacterial effects against four Gram-positive and Gram-negative bacterial strains, viz., Enterococcus faecalis, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. MTT assay was applied to determine the cytotoxic activity of GLME against PDL and Hek-293 normal cell lines and MCF-7 and K-562 cancer cell lines. The IC50 values of 598 µg mL-1 and 291 µg mL-1 were obtained for MCF-7 and K-562 cancer cell lines, which confirmed the ...
Microbial Physiology, 2015
The aim of this study was to conduct a molecular and biochemical characterization and to compare the antioxidant and antiproliferative activities of four Ganoderma isolates belonging to Ganoderma lucidum (Gl-4, Gl-5) and Ganoderma resinaceum (F-1, F-2) species. The molecular identification was performed by ITS and IGS sequence analyses and the biochemical characterization by enzymatic and proteomic approaches. The antioxidant activity of the ethanolic extracts was compared by three different methods and their flavonoid contents were also analyzed by high-performance liquid chromatography. The antiproliferative effect on U937 cells was determined by MTT assay. The studied mycelia differ both in the enzymatic activities and protein content. The highest content in total phenol and the highest antioxidant activity for DPPH free radical scavenging and chelating activity on Fe2+ were observed with the Gl-4 isolate of G. lucidum. The presence of quercetin, rutin, myricetin, and morin as ma...
Antioxidant and antimicrobial potential of Ganoderma lucidum and Trametes versicolor
Turkish Journal of Biochemistry
Objectives The majority of research programs had been focused on extract from the fruiting body. However, fungal mycelium obtained by submerged cultivation also has higher pharmacological potential. The aim of this study was investigation of the antioxidant and antimicrobial potential of Ganoderma lucidum and Trametes versicolor fungal mycelium biomass obtained by submerged cultivation and liquid fluid. Methods The antioxidant activity was evaluated by comparing the oxidation kinetics of the reduced form of 2,6-dichlorophenolindophenol with atmospheric oxygen in the presence/absence of biological samples. A criterion for evaluating for assessing the antioxidant activity was the values of the inhibition constant of the oxidation reaction. Antimicrobial activity of the fungal extracts was performed by agar disc diffusion method. Results A comparative analysis of antioxidant potential of G. lucidum and T. versicolor mycelium biomass and liquid fluid was conducted for the first time. It...
Antipathogenic efficacy of Ganoderma lucidum (Curtis) P. Krast
Disease and disorders are foremost cause of death in developing countries of the world. The present study has been carried out to evaluate the inhibitory impact of different extracts of Ganoderma lucidum on Staphylococcus aureus (MTCC 3160) and Salmonella typhi (MTCC 1249) by agar well diffusion and broth dilution method. The extracts contain proximate mycochemical such as phenols, alkaloids, saponines, flavonoids, terpenoids, polysaccharides etc. Methanolic extract showed higher MIC and ZOI (MIC: 50 μg/mL and ZOI: 2- 12mm) against S. aureus and S. typhi among all the tested extract in agar well diffusion method. In broth dilution method all extracts showed 100% inhibition.
Antioxidant Evaluation of Ganoderma lucidum Extracts
IOP Conference Series: Materials Science and Engineering, 2019
Ganoderma lucidum is one of the many mushrooms utilized by the people as a medicine for cancer, asthma, diabetes and hearth disease. The purpose of this experiment is to determine the antioxidant activity of the extracts from G. lucidum using different solvents. The simplicial powder of G. lucidum are subjected to maceration using water, methanol, ethyl acetate, methylene chloride, and n-hexane; and evaporated the solvent until a viscous extract was obtained. The methanol extract shows highest antioxidant activity with IC50 value of 251.95 μg/mL.
2020
Members of Ganoderma genus, belong to Basidiomycota, such as Ganoderma applanatum and Ganoderma lucidum have been noticed in traditional and modern medicine and pharmacology for their medicinal properties and bioactive compounds. The present study is undertaken to determine whether there is difference between chemical properties of G. applanatum and G. lucidum. The fresh mature fruiting bodies of fungi growing on common hornbeam (Carpinus betulus), were collected in Neka, a county in Mazandaran Province, Iran, and their antioxidant activity and bioactive compounds content were examined by spectrophotometer and HPLC method. According to the chemical analysis, the total phenols and flavonoids content, betulinic acid and also antioxidant activity measured by DPPH radical scavenging and FRAP methods in G. applanatum were higher than the other, but G. lucidum had higher content of total polysaccharides and proteins. Also the two other terpenoids (oleanolic acid and ursolic acid) were ver...