Study of Immune Response and Malondialdehyde Levels in Irradiated Rats Supplemented with Curcuma xanthorriza Roxb Extract (original) (raw)
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Gravity: Jurnal Ilmiah Penelitian dan Pembelajaran Fisika, 2020
The interaction of radiation with cells will form free radicals. Free radicals can cause cell damage. The presence of free radicals contained in cells could be detected using Electron Spin Resonance. Antioxidant compounds could minimize free radicals. This study analyzed curcumin extract and gamma radiation exposure on the area of free radical curves in the lungs of mice. Eighty male mice were used in this study and were divisible into three groups, namely the negative control group, the non-extract radiation group, and the extract plus radiation group. Curcumin extract was given at a dose of 1,4 gr/kgBB; 2,0 gr/kgBB; 2,6 gr/kgBB; 3,2 gr/kgBB and 3,8 gr/kgBB. The mice were exposed to gamma radiation for 10, 20, 30, 40, and 50 minutes. The results showed that curcumin extract, which was given as prevention from gamma radiation, reduced the area of the free radical curve from 0,630 cm 2 to 0,004 cm 2 .
The effect of curcumin on lipid peroxidation and selected antioxidants in irradiated rats
Acta Veterinaria Brno, 2018
The effect of irradiation on oxidants and antioxidants in selected tissues and a possible protective effect of curcumin on these indices were investigated. A total of 28 rats were divided into 3 groups; group 1 was control; group 2 was the irradiation group, saline was administered intraperiotenally (i.p.) for three days and then, 9 Gy gamma irradiation was applied; group 3 was the irradiation + curcumin group: curcumin was given i.p. for three days at 200 mg/kg body weight and then the same dose of irradiation was applied. A significant increase in malondialdehyde (MDA) was detected in the liver, kidney, and brain tissues of the rats as a result of irradiation (P < 0.01). Glutathione peroxidase (GSH-Px) activity in all the tissues (except for kidneys) decreased (P < 0.01), liver SOD (superoxide dismutase) activity decreased (P < 0.05), and GSH (glutathione) levels in kidney and ovary tissues (P < 0.001) significantly increased. While curcumin administration returned the...
TheScientificWorldJournal, 2014
Curcuma xanthorrhiza (CX) has been used for centuries in traditional system of medicine to treat several diseases such as hepatitis, liver complaints, and diabetes. It has been consumed as food supplement and "jamu" as a remedy for hepatitis. Hence, CX was further explored for its potential as a functional food for liver related diseases. As such, initiative was taken to evaluate the antioxidant and hepatoprotective potential of CX rhizome. Antioxidant activity of the standardized CX fractions was determined using in vitro assays. Hepatoprotective assay was conducted against carbon tetrachloride- (CCl4-) induced hepatic damage in rats at doses of 125, 250, and 500 mg/kg of hexane fraction. Highest antioxidant activity was found in hexane fraction. In the case of hepatoprotective activity, CX hexane fraction showed significant improvement in terms of a biochemical liver function, antioxidative liver enzymes, and lipid peroxidation activity. Good recovery was observed in the...
Inflammopharmacology, 2008
A comparison of crude curcuminoid extract and purifi ed curcumin was made to evaluate hepato-and immunoprotective effect of Curcuma longa (turmeric) Zingiberaceae. Carbon tetrachloride (CCl 4) induced cellular hepatic damage was evaluated by transmission electron microscopy, hepatic enzymes and thiobarbituric acid reactive species (TBAR) values. A selective cytolytic effect of CCl 4 was observed among immature (PNA +) thymocytes and peripheral helper (CD4 +) T lymphocytes in spleen and was paralleled by a signifi cant reduction in CD25, CD71 and Con A receptor expression. Treatment with curcuminoid crude extract at two different doses, showed a signifi cant cellular recovery among hepatocytes, which was refl ected in a reduction of hepatic enzymes and TBAR values. A signifi cant restoration of lymphocyte viability and CD25, CD71 and Con A receptor expression in both immature (PNA +) thymocytes and splenic helper (CD4 +) T lymphocytes was observed. Turmeric crude extract, at both low and high dose, was found to be more effi cient as compared to purifi ed curcumin.
Food and Chemical Toxicology, 1993
Curcuma xanthorrhiza Roxb., a medicinal plant used in Indonesia, has been shown to exert diverse physiological functions. However, little attention has been paid to its effect on lipid metabolism. We have investigated the effects of C. xanthorrhiza on serum and liver lipids, serum high density lipoprotein (HDL)-cholesterol and apolipoprotein (apo) A-I, and liver lipogenic enzymes in rats. In rats given a cholesterol-free diet, C. xanthorrhiza decreased the concentrations of serum triglycerides and phospholipids, and liver cholesterol, and increased serum HDL-cholesterol and apo A-I. The activity of liver fatty acid synthase, but not glycerophosphate dehydrogenase, was decreased by the medicinal plant. In rats on a high-cholesterol diet, C. xanthorrhiza did not suppress the elevation of serum cholesterol, although it did decrease liver cholesterol. Curcuminoids prepared from C. xanthorrhiza had no significant effects on the serum and liver lipids. These studies, therefore, indicate that C. xanthorrhiza contains an active principle(s) other than curcuminoids which can modify the metabolism of lipids and lipoproteins.
Cancer chemoprotective effects ofCurcuma xanthorrhiza
Phytotherapy Research, 2008
Asia. In particular, it is commonly used not only for food and medicinal purposes in Indonesia, but also for the topical treatment of acne and skin inflammations as Thai traditional medicine. It was found that the methanol extract of C. xanthorrhiza inhibited significantly 7,12-dimethylbenz[a]anthracene (DMBA)-induced bacterial mutagenesis of Salmonella typhimurium TA98 and TA100 in the presence of S9, and the mutagenesis induced by H 2 O 2 and tert-butylhydroperoxide in S. typhimurium TA102, respectively. In addition, 12-O-tetradecanolyphorbol-13-acetate(TPA)-induced mouse ear edema was markedly inhibited by pretreatment with C. xanthorrhiza extract. C. xanthorrhiza extract dose-dependently reduced ODC expression in mouse skin with TPA-induced acute inflammation. Furthermore, repeated treatment with 0.1% C. xanthorrhiza extract reduced the average number of tumors per mouse and the percentage of tumor-bearing mice in a multistage mouse skin carcinogenesis induced by DMBA and TPA. These results demonstrate that the methanol extract of C. xanthorrhiza possesses cancer chemopreventive potential.
Journal of Radiation Research, 2007
The et:fect of mononuclear copper (II) complex of curcumin in 1:1 stoiehiometry (hereafter referred to as complex) administered 30 min before y-irradiation (4.5 Gy) on alterations in antioxidant and Thiobarbituric acid reactive substances (TBARS) levels in iivers was studied in comparison to curcumin at a dose of 50 mgfkg. The different antioxidants like GSH, GST, catalase, SOD, TBARS and total thiois were estimated in the liver homogenates excised at different time intervals (1, 2 and 4 h) post irradiation using colorimetric methods. There wus a radiation-induced decrease in the levels of all the studied enzymes at 1 h post irradiation, while an increase was observed at later time points. Both curcumin and complex treatment in sham-irradiated mice decreased the levels of GSH and total thiols, whereas there was an increase in the levels of catalasc, GST and SOD compared to normal contre]. Under the infiuence of irradiation, both curcumin and complex treatment protected the deeline in the levels of GSH, GST, SOD, catalase and total thiols, and inhibited radiation-induced lipid peroxidation. Further, the complex was found to be more effective in protecting the enzymes at 1 h post irradiation compared to curcumin treated group. This may be due to the higher rate constants of the complex compared to curcumin fbr their reactions with various free radicals.
Experimental and Toxicologic Pathology, 2011
The effect of dried fruit extract from Xylopia aethiopica (Annonaceae) (XA) and vitamin C (VC) against ␥-radiation-induced liver and kidney damage was studied in male Wistar rats. XA and VC were given orally at a dose of 250 mg/kg, orally for 6 weeks prior to and 8 weeks after radiation (5 Gy). The rats were sacrificed after 1 and 8 weeks of single exposure to radiation. Results showed that all animals in un-irradiated group survived (100%), while 83.3% and 66.7% survived in XA-and VC-treated groups, respectively, and 50% survived in irradiated group. The levels of serum, liver and kidney lipid peroxidation (LPO) were elevated by 88%, 102% and 73% after 1 week of exposure, and by 152%, 221% and 178%, after 8 weeks of exposure, respectively. Treatment with XA and VC significantly (p < 0.05) decreased the levels of LPO in the irradiated animals. Also, ␥-radiation caused significant decreases (p < 0.05) in the levels of liver glutathione (GSH), glutathione-S-transferase (GST), catalase (CAT), superoxide dismutase (SOD), kidney GSH and SOD by 41%, 60%, 81%, 79%, 72% and 58% after 1 week of exposure. Similarly, ␥-radiation caused significant increases (p < 0.05) in the levels of serum alanine (ALT) and aspartate aminotransferases (AST) after 8 weeks of exposure. Precisely, ALT and AST levels were increased by 69% and 82%, respectively. These changes were significantly (p < 0.05) attenuated in irradiated animals treated with XA and VC. These results suggest that XA and VC could increase the antioxidant defence systems in the liver and kidney of irradiated animals, and may protect from adverse effects of whole body radiation.
Curcumin Stimulates the Antioxidant Mechanisms in Mouse Skin Exposed to Fractionated γ-Irradiation
Antioxidants, 2015
Fractionated irradiation is one of the important radiotherapy regimens to treat different types of neoplasia. Despite of the immense therapeutic gains accrued by delivering fractionated irradiation to tumors, the radiation burden on skin increases significantly. Low doses of irradiation to skin adversely affect its molecular and metabolic status. The use of antioxidant/s may help to alleviate the radiation-induced changes in the skin and allow delivering a higher dose of radiation to attain better therapeutic gains. Curcumin is an antioxidant and a free radical scavenging dietary supplement, commonly used as a flavoring agent in curries. Therefore, the effect of 100 mg/kg body weight curcumin was studied on the antioxidant status of mice skin exposed to a total dose of 10, 20 and 40 Gy γ-radiation below the rib cage delivered as a single fraction of 2 Gy per day for 5, 10 or 20 days. Skin biopsies from both the curcumin treated or untreated irradiated groups were collected for the biochemical estimations at various post-irradiation times. The irradiation of animals caused a dose dependent decline in the glutathione concentration, glutathione peroxidase, and superoxide dismutase activities and increased the lipid peroxidation in the irradiated skin. Curcumin treatment before irradiation resulted in a significant rise in the glutathione concentration and activities of both the glutathione peroxidase and superoxide dismutase enzymes in mouse skin, whereas lipid peroxidation declined significantly. The present study indicates that curcumin treatment increased the antioxidant status of mouse exposed to different doses of fractionated γ-radiation.
Journal of Ethnopharmacology, 2010
Aim of the study: To investigate the protective effect and possible mechanism of Curcuma comosa hexane extract on CCl 4 -induced liver injury in adult male mice. Materials and methods: Hepatotoxicity was induced by an intraperitoneal injection of CCl 4 and was evaluated after 24 h from the elevations of plasma alanine transaminase (ALT) and aspartate transaminase (AST) activities, and histological analysis of liver injuries. Hexane extract of Curcuma comosa was given at different time points from 1 to 72 h, prior to CCl 4 administration and the protection from liver injury was assessed. Results: CCl 4 -induced damage to liver cells was resulted in elevations of plasma ALT and AST activities. Pretreatment with Curcuma comosa hexane extract 24 h at a dose of 100, 250, and 500 mg/kg BW resulted in a dose-dependent prevention of the increases in plasma ALT and AST activities as well as time dependent. The protective effect of the extract at a dose of 500 mg/kg BW was seen at 12-24 h. Pretreatment of the extract completely prevented elevation of plasma ALT and AST activities, and centrilobular necrosis. The protective effect of Curcuma comosa was associated with restoration of hepatic glutathione content, and CYP2E1 catalytic activity, and its mRNA and protein levels as well as increase in activity of glutathione-S-transferase (GST). Conclusion: Curcuma comosa has a potent protective property against CCl 4 -induced hepatic injuries via the activation of detoxifying mechanisms (GST) as well as reduction of the bioactive toxic metabolites. Therefore, Curcuma comosa may be beneficial for prevention of hepatotoxicity.