DNA-templated assembly of nanoscale architectures (original) (raw)

DNA-templated assembly of nanoscale architectures for next-generation electronic devices

Faraday Discussions, 2006

We report the assembly and structural characterization of a Y-shaped DNA template incorporating a central biotin moiety. We also report that this template may be used to assemble nanoscale architectures, which demonstrate the potential of this and related approaches to the fabrication of next-generation electronic devices. Of particular significance is the finding that it is possible to selectively metallize the above DNA template to obtain a three-electrode configuration. Also of particular significance is the finding that a biotin modified nanoparticle will recognize and bind selectively the central biotin moiety of the same template, once functionalized by the protein streptavidin.

Self-Assembled DNA-Based Structures for Nanoelectronics

Journal of Self-Assembly and Molecular Electronics, 2013

Recent developments in structural DNA nanotechnology have made complex and spatially exactly controlled self-assembled DNA nanoarchitectures widely accessible. The available methods enable large variety of different possible shapes combined with the possibility of using DNA structures as templates for high-resolution patterning of nano-objects, thus opening up various opportunities for diverse nanotechnological applications. These DNA motifs possess enormous possibilities to be exploited in realization of molecular scale sensors and electronic devices, and thus, could enable further miniaturization of electronics. However, there are arguably two main issues on making use of DNA-based electronics: (1) incorporation of individual DNA designs into larger extrinsic systems is rather challenging, and (2) electrical properties of DNA molecules and the utilizable DNA templates themselves, are not yet fully understood. This review focuses on the above mentioned issues and also briefly summarizes the potential applications of DNA-based electronic devices.

Templates for sequential assembly of DNA based nanostructures

5th IEEE Conference on Nanotechnology, 2005., 2005

Elements of the design, synthesis, cloning, amplification, isolation and characterization of template strands of DNA applicable to the parallel construction of nanostructures via sequential assembly processes are described. Particularly, codes have been filed within bacteria which can be accessed to obtain one micron long single stranded DNA molecules which contain multiple copies of a 32nm repetitive sequence. Characterization of these template strands has been performed using Atomic Force Microscopy.

Electronic nanostructures templated on self-assembled DNA scaffolds

Nanotechnology, 2004

We report on the self-assembly of one-and two-dimensional DNA scaffolds, which serve as templates for the targeted deposition of ordered nanoparticles and molecular arrays. The DNA nanostructures are easy to reprogram, and we demonstrate two distinct conformations: sheets and tubes. The DNA tubes and individual DNA molecules are metallized in solution to produce ultra-thin metal wires.

Overview of New Structures for DNA-Based Nanofabrication and Computation

2000

Summary This paper presents an overview of recent experimental progress by the Duke DNA NanoTech Group in our efforts to utilize novel DNA nanostructures for computational self-assembly as well as for templates in the fabrication of functional nano-patterned materials. We have prototyped a new DNA tile type known as the 4x4 (a cross-like structure composed of four four-arm junctions) upon

Connecting the Nanodots: Programmable Nanofabrication of Fused Metal Shapes on DNA Templates

Nano letters, 2011

b S Supporting Information N anoscale metallic structures hold great potential for both electronic and plasmonic applications. Their construction requires the controlled placement of metal with nanometer resolution and the ability to couple to the resulting structures, either electrically or optically. Fabrication of such structures is challenging and has pushed current lithographic techniques to their limits. For this reason, scientists have sought to selfassemble such nanostructures rather than use the conventional top-down approaches.

A polycatenated DNA scaffold for the one-step assembly of hierarchical nanostructures

Proceedings of the National Academy of Sciences, 2008

A unique DNA scaffold was prepared for the one-step self-assembly of hierarchical nanostructures onto which multiple proteins or nanoparticles are positioned on a single template with precise relative spatial orientation. The architecture is a topologically complex ladder-shaped polycatenane in which the ''rungs'' of the ladder are used to bring together the individual rings of the mechanically interlocked structure, and the ''rails'' are available for hierarchical assembly, whose effectiveness has been demonstrated with proteins, complementary DNA, and gold nanoparticles. The ability of this template to form from linear monomers and simultaneously bind two proteins was demonstrated by chemical force microscopy, transmission electron microscopy, and confocal fluorescence microscopy. Finally, fluorescence resonance energy transfer between adjacent fluorophores confirmed the programmed spatial arrangement between two different nanomaterials. DNA templates that bring together multiple nanostructures with precise spatial control have applications in catalysis, biosensing, and nanomaterials design.

Bottom-Up Fabrication of DNA-Templated Electronic Nanomaterials and Their Characterization

Nanomaterials

Bottom-up fabrication using DNA is a promising approach for the creation of nanoarchitectures. Accordingly, nanomaterials with specific electronic, photonic, or other functions are precisely and programmably positioned on DNA nanostructures from a disordered collection of smaller parts. These self-assembled structures offer significant potential in many domains such as sensing, drug delivery, and electronic device manufacturing. This review describes recent progress in organizing nanoscale morphologies of metals, semiconductors, and carbon nanotubes using DNA templates. We describe common substrates, DNA templates, seeding, plating, nanomaterial placement, and methods for structural and electrical characterization. Finally, our outlook for DNA-enabled bottom-up nanofabrication of materials is presented.

ChemInform Abstract: DNA Nanoarchitectures: Steps Towards Biological Applications

ChemInform, 2014

DNA's remarkable molecular recognition properties, flexibility and structural features make it one of the most promising scaffolds to design a variety of nanostructures. During the past decades, two major methods have been developed for the construction of DNA nanomaterials in a programmable way, both generating nanostructures in one, two and three dimensions: the tile-based assembly process, which provides a useful tool to construct large and simple structures, and the DNA origami method, suitable for the production of smaller, more sophisticated and well defined structures. Proteins, nanoparticles and other functional elements have been specifically positioned into designed patterns on these structures. They can also act as templates to study chemical reactions, help in the structural determination of proteins and be used as platform for genomic and drug delivery applications. In this review we examine recent progresses towards the potential use of DNA nanostructures for molecular and cellular biology.

DNA-programmed assembly of nanostructures

Organic & Biomolecular Chemistry, 2005

DNA is a unique material for nanotechnology since it is possible to use base sequences to encode instructions for assembly in a predetermined fashion at the nanometre scale. Synthetic oligonucleotides are readily obtained by automated synthesis and numerous techniques have been developed for conjugating DNA with other materials. The exact spatial positioning of materials is crucial for the future development of complex nanodevices and the emerging field of DNA-nanotechnology is now exploring DNA-programmed processes for the assembly of organic compounds, biomolecules, and inorganic materials. h e m i s t r y 2 0 0 5 O r g . B i o m o l . C h e m . , 2 0 0 5 , 3 , 4 0 2 3 -4 0 3 7 4 0 2 3