Quantitative Proteomic Analysis of Histone Modifications (original) (raw)

In eukaryotic cells, chromosomal DNA is packaged into a compact structure, chromatin, with the use of four core histones (H2A, H2B, H3, and H4). The fundamental repeating unit of chromatin is the nucleosome, which is composed of an octamer of the core histones, around which ~147 base pairs of DNA are wrapped. Nucleosomes are in turn folded into progressively higher-order structures. Dynamic chromatin remodeling plays a critical role in regulating diverse DNA-based biological processes, such as transcription of RNA, DNA replication, and DNA repair, as well as chromosome condensation and segregation. 1 *