Association of kisspeptin in patients with poly cystic ovarian syndrome (original) (raw)
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Gynecological Endocrinology, 2020
Objective: To determine serum kisspeptin and anti-mullerian hormone (AMH) levels in women with and without polycystic ovary syndrome (PCOS). Materials and methods: This was a prospective, cross-sectional, comparative study that included 70 women with PCOS and 58 non PCOS controls. PCOS patients were diagnosed according to the Rotterdam criteria. Age, body mass index (BMI), number of menstrual cycles per year, and the Ferriman-Gallwey Score were determined for each woman. Serum levels of kisspeptin, follicle stimulating hormone (FSH), luteinizing hormone (LH), thyroid stimulating hormone (TSH), estradiol, total testosterone, dehydroepiandrosterone sulfate (DHEA-S), AMH, fasting glucose and insulin were determined. Results: Women with PCOS were younger (p < .001), with higher BMI (p ¼ .027) and glucose values (p < .001); while displaying less number of menstrual cycles per year (p < .001). Although serum kisspeptin levels were similar in both groups, age was negatively (r¼ À0.33, p ¼ .00018) and serum AMH levels were positively correlated (r ¼ 0.25, p ¼ .0039) with the serum kisspeptin levels in women with the PCOS. After adjusting for age, serum kisspeptin levels were comparable in both groups (p > .05). Serum LH, AMH, DHEA-S and total testosterone glucose, insulin levels and HOMA-IR values were significantly higher in women with PCOS as compared to controls (all p < .05). Conclusions: Serum kisspeptin levels were similar in women with and without PCOS but positively correlated with AMH serum levels in PCOS women.
Circulating Kisspeptin Levels Regulate LH Rise in PCOS
2020
The study was planned to determine the relationship between serum kisspeptin levels and LH elevation in PCOS patients. Materials and Methods: A total of 40 women were diveded in to two groups as PCOS and control. Patients in PCOS group consisted of 20 women. The control group consisted of 20 BMI-matched women without PCOS. Women in the control group were randomly selected from patients admitted with our clinic for routine gynecological examination. Venous blood samples for hormone, glucose and insulin analysis were taken following a 12 hour fasting during the early follicular phase of spontaneous or progesterone withdrawal bleeding. Serum kisspeptin levels were measured by using the Human ELISA kit. Results: The kisspeptin values of PCOS patients were found to be significantly higher than the control group (p < 0.01). Serum testosterone, LH, insulin and HOMA-IR of PCOS patients were significantly higher than the control group (p < 0.02, p < 0.01, p < 0.03 and p < 0.01, respectively). Serum kisspeptin levels are positively correlated with the serum levels of LH (r = 0.644, p < 0.01) and negatively correlated with HOMA-IR (r = 0.431, p < 0.02). Conclusion: The increase in serum kisspeptin levels is one of the reasons for the increase of LH in PCOS patients.
The Correlation between Hormonal Disturbance in PCOS Women and Serum Level of Kisspeptin
International Journal of Endocrinology, 2020
Background. Kisspeptin is a neuropeptide that upregulates gonadotropin-releasing hormone (GnRH) secretion. It is an essential element for the luteinizing hormone (LH) surge and ovulation. Women with polycystic ovary syndrome (PCOS) expose alteration in both GnRH and LH secretion levels. Objective. is paper aims to evaluate serum kisspeptin levels in healthy and polycystic ovarian syndrome women. Furthermore, it investigates the effect of obesity and age on circulating kisspeptin levels in both normal and PCOS women. Moreover, it points out the correlation between kisspeptin and other hormonal parameters. Methods and Patients. One hundred women (60 are with PCOS and 40 are normal) were enrolled in the study. Five milliliter samples of blood from all the patients and control women were obtained twice during the menstrual cycle. All the study samples were classified depending on the age factor for several subgroups. Results. Kisspeptin levels were higher in PCOS patients than those in the normal group. Kisspeptin correlated with serum free testosterone level (r � 0.26). In healthy women, preovulatory kisspeptin levels were higher than follicular kisspeptin levels (P < 0.05), while this difference was insignificant in PCOS patients. e variation in serum kisspeptin levels between overweight/obese and normal-weight women was insignificant. In normal women, serum kisspeptin levels were higher in women >35 years than those <24 years at (P � 0.03). Conclusion. e serum kisspeptin level is higher in PCOS women. Its levels fluctuate during the menstrual cycle, but these fluctuations are disturbed in PCOS women. e effect of BMI on serum kisspeptin levels is insignificant, and kisspeptin serum levels increase with age.
Journal of Advanced Biotechnology and Experimental Therapeutics, 2022
Polycystic ovary syndrome (PCOS) is a heterogeneous genetic disorder categorized by hyperandrogenism that affects early reproductive age in females. KISS1 has played role in regulating the hypothalamic-pituitary-gonad axis. It also plays a key role in human reproductive function. Imbalance-of-function mutations is often found KISS1 gene of patients with polycystic ovary syndrome. Blood samples were collected from 120 patients (60 control are divided into 30 normal weight and 30 obese) and (60 PCOS) females are divided into 30 normal weight and 30 obese. DNA was extracted and genotyped for KISS1 variants by HRM-PCR and measured the level of kisspeptin by ELIAS, while LH, FSH, DHEA and free testosterone by CLIA. The value of LH, Testosterone, DHEA-S and kisspeptin is elevated in the patient group, while the decline of FSH in serum level patients value, rs372790354 G > A and rs4889 G>A was associated with PCOS in dominant, recessive, co-dominant (P-value< 0.05), rs37279054 AA was not found the effect of obese group and linked with normal weight PCOS put present study no effect on the parameters, rs4889 GG/GA was the effect on all subgroups except the genotype GA not effected on obese female, the highly significant (P-value<0.05) of rs4889 GA influenced on measured of WHR, LH/FSH ratio and DHEA-S in the patient compared to control, rs4889 GG/AA was influenced on normal-weight patient compare to an obese patient, the WHR was higher in an obese patient in both genotype. While the level of kisspeptin in normal weight with genotype AA was higher level compared to obese and (P-value<0.05). We concluded that the KISS1 levels were higher in PCOS females compared to controls and decreased with increasing BMI, KISS1 polymorphism rs372790354 G>A and rs4889 G>A may be associated with the pathophysiology of PCOS and lead to increased serum level of LH that due to hyperandrogenism.
The role of Kisspeptin levels in polycystic ovary syndrome: a systematic review and meta-analysis
Arch Gynecol Obstet., 2019
PURPOSE: Polycystic ovarian syndrome (PCOS) is a complex and not fully elucidated pathology. This prevalent endocrinopathy affects patients in reproductive age, impacts on estrogen-dependent diseases, as well as in infertility. In this context, Kisspeptin (KP) may be considered a potential biomarker for PCOS diagnosis and follow-up. Here, we aimed to verify the levels of KP in obese and non-obese patients with PCOS, their relationship with other hormones, in comparison to healthy controls. METHODS: A systematic review and meta-analysis were performed according to the PRISMA guidelines. We searched MEDLINE, EMBASE, PsycINFO, Global Health, The Cochrane Library, Health Technology Assessment Database, and Web of Science for eligible studies. A random effects model meta-analysis of standardized mean difference (SMD) was conducted and the I2 was used to assess heterogeneity. Meta-regression was conducted through mixed-effects model. RESULTS: A total of 12 studies were included, comprising 660 PCOS patients and 600 controls. The KP levels were lower in the control group (0.76: 0.17-1.35; 95% CI). In the subgroup analyses, patients were divided in non-overweight/obese (BMI < 25) and overweight/obese (BMI ≥ 25) groups. The meta-regression revealed a difference between the obese and non-obese groups (z = 2.81; p = 0.0050). CONCLUSIONS: PCOS patients showed higher KP levels than control, and obese non-PCOS patients also showed altered KP levels. All studies had poor descriptions of sample collection, pre-analytical and analytical procedures, which is critical considering structural characteristics of the KP molecule.
Role of kisspeptin in polycystic ovary disease in patients of ALNajaf ALAshrif City, Iraq
Journal of pharmaceutical science and research , 2019
The aim of the study was to arrive at some biochemical and hormonal evidence predicting ovarian polycystic ovaries in women with primary or secondary infertility. Samples were collected at the fertility center laboratories in Sadr Medical City, Najaf, Iraq. The blood was taken for complete blood cell (CBC) testing on the samples. Samples were taken 88 samples (70 of which were samples of women with polycystic ovaries and 18 sample of control women). A serum was then taken for biochemical tests, The hormones LH, FSH, prolactin, Estradiol, Kisspeptin1, Interleukin 37 were measured by ELISA method while lipid profile and total protein by spectrophotometer method. The result showed a significant increase (p<0.05) of Kisspeptin-1 ,Prolactin, cholesterol ,triglyceride ,low density lipoprotein ,very low density lipoprotein, total protein and RBC in women with polycystic ovary disease compared with control group, While significant decrease (p<0.05) of high density lipoprotein and Interleukin-37 in women with polycystic disease compared with control group. And the results showed non-significant (p > 0.05) in both Luteinizing hormone, Follicle stimulation hormone, Estradiol hormone, platelet, WBC. The significant increase in the level of Kisspeptin and some of the biochemical and physiological parameters measured in the present study may provide an indicator of increased infertility in women with ovarian disease.
Kisspeptin And Galanin-like Peptide (GALP) Levels In Women With Polycystic Ovary Syndrome
2016
The aim of the study was to compare kisspeptin and GALP levels between women with and without PCOS and to analyze the correlation between kisspeptin, GALP and PCOS-related hormonal and metabolic disturbances. Patients and methods: The study included 87 PCOS women, and a control group of 42 healthy women with a regular menstrual pattern and no signs of hyperandrogenisms. Circulating levels of luteinizing hormone (LH), follicle-stimulating hormone (FSH), testosterone (T), sex-hormone binding globulin (SHBG), thyroid stimulating hormone (TSH), prolactin, fasting insulin, kisspeptin and GALP were measured. Results: Kisspeptin (P<0.05) and GALP (P>0.05) levels were significantly higher in the PCOS women than in the controls. In the normal weight PCOS women kisspeptin correlated positively with GALP (P<0.01), waist circumference (WC) (P<0.05) and free androgen index (FAI) (P<0.05) and negatively with fasting glucose (P<0.05). In this group GALP correlated positively with...
Diabetes & Metabolic Syndrome: Clinical Research & Reviews, 2019
Background: Polycystic ovary syndrome (PCOS) is a heterogeneous disorder characterized by a diverse collection of reproductive and metabolic abnormalities. kisspeptin (KISS) is novel peptides associated with regulation of metabolism, food intake, puberty and reproduction. The aim of the present study was to estimate KISS level in patients with PCOS, and to evaluate the possible relationship between KISS level with anthropometric measures as well as clinic-morphological features of PCOS. Materials and methods: cross section control study enrolled 90 control group and 105 patients with PCOS and they were stratified according to their body mass index (BMI) to; underweight (n ¼ 9, BMI ˂19), normal weight (n ¼ 25, BMI ¼ 19.1e25), over weight (n ¼ 34,BMI ¼ 25.1e30), obese grade I (n ¼ 12, BMI ¼ 30.1e35) , obese grade II (n ¼ 13, BMI 35.1e40) and obese grade III (n ¼ 12, BMI˃40).Circulating KISS levels were measured using ELISA. Results: Our results revealed that, KISS levels were higher in PCOS patients compared to controls. Among PCOS group, there were significant lower level of KISS levels in underweight, overweight and obese compared to normal weight group. Even more importantly, KISS levels decreased with increasing of BMI as the following, grade I, grade II and grade III. Moreover, it was negatively correlated to anthropometric measures, glycemic, lipid profile and positively correlated the phenotype characteristics of PCOS. Linear regression test observed that hirsutism score, HOMA-IR and LH were the main predictors of KISS levels in PCOS. Conclusion: circulating KISS is an important regulator of body weight and reproduction especially in PCOS women.
Journal of biomedical science, 2018
Kisspeptin is involved in female reproduction. This study was designed to i- estimate kisspeptin levels in women with polycystic ovary syndrome (PCOS), in comparison with controls, ii- study the correlations between kisspeptin and PCOS-related reproductive hormones, and iii- investigate the relation between KISS1 gene polymorphisms and hormone levels in women suffering from PCOS. The investigation was a clinically designed study on 28 women with PCOS, and 30 normal, healthy women with no signs of PCOS as controls. Blood samples were collected between day 3 and day 6 of the menstrual cycle in both groups at 8:00 a.m., and circulating levels of LH, FSH and kisspeptin were estimated. DNA was extracted from whole blood and all coding exons of KISS1 gene were sequenced. Women with PCOS had higher LH levels and BMI compared to controls. Plasma kisspeptin levels were positively correlated with LH levels. There was no statistically significant difference between the groups in terms of kissp...
Journal of Pharmaceutical Negative Results
background: (PCOS) is among the most common types of gynecological hormonal abnormalities and metabolic problems that have an impact females throughout adolescence, with a prevalence of 6-10%.Hyperandrogenism, unreliable menstrual periods, and polycystic ovaries are the hallmarks of PCOS. The (HPG) axis is controlled by kisspeptins, peptides made by the KISS1 gene.Objectives: This investigation's objective about used to be to analyze the affiliation of kiss1 Gene SNPs (rs 12998 ) with Endocrine Parameters in PCOS .Methodology: A case -control find out about enrolled one hundred sixty members of which eighty female have PCOS and eighty symbolize the manage crew who had-been interestingly healthful. Patients with PCOS had been chosen patients from our health center and the teaching hospital's gynecology and obstetrics department in Tikrit. Assessment of serum ( kisspeptin, estradiol ,LH, FSH and testosterone) used to be performed the use of ELISA approach and the maker company...