P126 Effect of different materials on the proliferation and migration of articular chondrocytes (original) (raw)

Purpose: Cell viability and matrix accumulation in scaffolds are important measurement outcomes of engineering cartilage tissues in vitro. Early assessments that predict longer-term outcomes would aid in more rapid screening of multiple scaffold-cell combinations. The objective of this study was to measure media metabolites for multiple chondrocyte-laden scaffolds at very early time points in culture, to evaluate their ability to predict extracellular matrix accumulation in the same samples at longer times. Methods and Materials: Porcine chondrocytes were encapsulated in 16 distinct formulations of a crosslinked elastin-like polypeptide (ELP). Constructs were cultured for 28 days, and culture media and pyruvate. On day 28, samples were assayed for accumulated sGAG. Regressions for accumulated sGAG on each metabolite were P126 Effect of different materials on the proliferation and migration of articular chondrocytes.