Organ- and cell cycle-dependent expression of genes (original) (raw)

2011

Abstract

<b>Copyright information:</b>Taken from "The genes encoding ORC subunits are E2F targets and the two genes are differently expressed in proliferating and endoreplicating cells"Nucleic Acids Research 2005;33(17):5404-5414.Published online 22 Sep 2005PMCID:PMC1236721.© The Author 2005. Published by Oxford University Press. All rights reserved () Expression pattern of genes in different organs. Measurements were normalized to the amount of or and, then all the values made relative to the amount of present in the sample of aerial part of these seedlings (the lowest of all). Samples were prepared from aerial parts and root system of 12 day-old seedlings, young and mature rosette leaves, cauline leaves, stems, flowers at different stages or growth. () MM2d suspension cultured cells were sucrose-starved for 24 h and the amount of different AtORC mRNAs was determined by real-time RT–PCR, using the normalization procedure described for panel A. () MM2d suspension cultured cells, sucrose-starved for 24 h, were stimulated to re-enter the cell cycle, as described (). The amount of mRNA of several cell cycle marker genes () was determined at the indicated times after sucrose addition by real-time RT–PCR, as described in panel A. and were used as G1 markers, and histone , as S-phase markers and , as a G2/M marker (panel C). The mRNA levels of each gene (panel D) were determined at the indicated times after sucrose addition by real-time RT–PCR, as described in panel A. Numbers on top of the bars in panels C and D indicate the fold increase at the maximum level of expression relative to the value, in each case, obtained at time zero (arrested cells). In all cases, the RT–PCR measurements were repeated, at least, 2–3 times but error bars have been omitted for simplicity.

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