Histopathological Analysis and Comparison between Experimentally Fabricated Hydroxyapatite from Nile Tilapia Bone and Mineral Trioxide Aggregate as A Direct Pulp Capping Agents on Dog Pulp (In Vivo Study) (original) (raw)
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Summary: Aim: The aim of this study is to observe the inflammatory response and dentinogenesis following direct pulp capping with three different materials and to see the differences in the reparative dental hard tissue regarding its quality characteristics. Materials and Methods: For the purpose of this study we used experimental animals according to Ordinance №15 since 3th, Feb., 2006 on the minimal requirements for protection and welfare of laboratory animals. The animals were two-month old male Wistar rats. They were conducted under general anaesthesia . Then on the first upper molars were prepared first class cavities until reaching pulp exposure. The prepared teeth were divided into three groups according to the pulp-capping material- Enamel Matrix Derivative (EMD), Beta-tricalcium phosphate and Calcium Hydroxide. All of the teeth were filled with glass-ionomer cement (Ketac Molar Easymix, 3M Espe). 8, 15 and 30 days after the procedure the rats were euthanized under anaesthes...
The aim of this study was to evaluate the effect of two different pulp capping materials regarding dentin bridge formation after direct pulp capping for one week and two months. A total of 32 teeth were prepared for this study using two male dogs. Class V cavities were prepared on the buccal surface then exposure was done using a small round bur. Teeth were randomly divided into two main groups according to the applied capping materials (n=16); group I: MTA and group II: experimental Propolis paste. Prepared cavities were finally restored using resin modified glass ionomer. Then these two main groups were further divided into two subgroups (n=8) according to the observation period; one week and two months. Results revealed that no dentin bridge was formed after one week with both tested materials. On the other hand at two months observation period; there was dentin bridge formation in both MTA and Propolis groups and the difference was statistically insignificant. There was no statistically significant difference between both materials at each observation period. Experimental Propolis pulp capping material was able to induce reparative dentin bridge formation after two months. Experimental Propolis paste and MTA are successful direct pulp capping materials regarding dentin bridge formation.
Regenerative engineering and translational medicine, 2020
Dentin has been considered a promising scaffold for bone regeneration. This study investigated the effects of two different demineralization and deproteinization methods on the mechanical and physicochemical properties of dentin as a potential scaffold for tissue engineering approaches. Eighty dentin discs were divided into five groups according to the treatment process: control (C), no treatment; DEM1, complete demineralization with HCl; DEM2, partial demineralization with different concentrations of EDTA; DEP1, complete deproteinization using NaOCl solution; and DEP2, partial deproteinization by boiling water. The treated dentin discs were characterized using ATR-FTIR and SEM. The compressive strength, elastic modulus, and microhardness values of all C and treated samples were measured. They were analyzed using one-way ANOVA, Kruskal-Wallis, and Weibull analysis. FTIR showed significantly reduced mineral/matrix ratio in demineralized groups (DEM1 and 2) (p < 0.001), while increase of mineral/matrix ratio in DEP1 was not significant (p = 0.31). SEM observations revealed open dentinal tubules in DEM1 group and lower amounts in the other groups. The C showed the highest compressive strength (78.18 ± 16.19 MPa), and the DEM1 showed the lowest (0.84 ± 0.32 MPa). The treated groups showed lower Weibull moduli (m), when compared with the C group. The DEP2 (642 ± 318.9) and DEM1 (2.9 ± 1.37) groups showed the highest and lowest modulus of elasticity, respectively. DEM1 showed the least hardness values (10.27 ± 3.09) compared to the other groups (p < 0.001). Based on the results, the DEM1 showed confirmed demineralization and lowest mechanical properties. The highest mechanical properties belonged to DEM2 and DEP2 groups which both were partially treated. Depending on the purpose of the regeneration, the dentin processing method can be selected. Whenever the high mechanical properties are more important in scaffold selection, DEM2 and DEP2 are the best choices. Lay Summary This study investigated the effects of two different demineralization and deproteinization methods including DEM1, demineralization with HCl; DEM2, demineralization with EDTA; DEP1, deproteinization using NaOCl solution; and DEP2, deproteinization by boiling water on the mechanical and physicochemical properties of dentin as a potential scaffold. Based on the results, the DEM1 showed confirmed demineralization and lowest mechanical properties. The highest mechanical properties belonged to DEM2 and DEP2 groups which both were partially treated. Depending on the purpose of the regeneration, the dentin processing method can be selected. Whenever the high mechanical properties are more important in scaffold selection, DEM2 and DEP2 are the best choices. Future studies should be conducted to assess the osteoinductive/osteoconductive potential Yasaman Erfan and Tahereh Sadat Jafarzadeh Kashi contributed equally to this work.
Aim Find a reasonable substitution for current DPC materials. Methods The hTDM properties were evaluated by assessing its alkalinity, antibacterial activity, and potentiality to induce cell migration compared to MTA. The acute inflammatory response was evaluated 3 days after iatrogenic pulp exposure by histological examination. However, both chronic inflammatory response and dentin-pulp complex regeneration were evaluated after 3 months. Results The results of in vitro assessment revealed high alkalinity of MTA while hTDM exhibited optimum alkalinity. Moreover, the higher antibacterial activity and potentiality of inducing cell migration than MTA. The in vivo assessment ensured the ability of hTDM in optimizing the inflammatory response and dentin-pulp complex regeneration. However, MTA prolonged the inflammatory response and induced the formation of a thick calcific bridge toward the pulp tissue. Conclusion hTDM semi-rigid scaffold is a promising novel DPC material.
Al-Azhar Journal of Dental Science, 2021
Objectives: The purpose of this study was to evaluate the pulpal response of dogs' teeth after direct pulp capping using Biodentine (BD) and compared it with Mineral Trioxide Aggregate (MTA). Materials and Methods: Following the splitmouth design, forty intact teeth in two healthy Mongrel dogs were randomly assigned to two experimental groups; group I: BD and group II: MTA. Standardized Class V cavities were prepared on the vestibular surface of each tooth where the pulp exposure was performed with a dental explorer. The pulp-exposed teeth were immediately capped with one of the tested materials. The prepared cavities were then finally restored with glass-ionomer cement. After termination of the observation periods (one week and three months), the animals were euthanized. Then, teeth were extracted for histopathological evaluations. Data collected and statistically analyzed by using Fisher's exact test. The significance level was set at P ≤ 0.05. Results: Histopathological analysis showed complete dentin bridge formation and an absence of inflammatory pulp response. Statistical analysis showed no significant differences between the BD and MTA experimental groups during the observation periods. However, a significantly higher thickness of the dentin bridge was found in the group of teeth treated with BD at three months. Conclusion: BD may be considered an interesting alternative to MTA. Both materials produced favorable pulpal responses that were similar in nature
In vitro reparative dentin: a biochemical and morphological study
European Journal of Histochemistry, 2013
In this study, starting from human dental pulp cells cultured in vitro, we simulated reparative dentinogenesis using a medium supplemented with different odontogenic inductors. The differentiation of dental pulp cells in odontoblast-like cells was evaluated by means of staining, and ultramorphological, biochemical and biomolecular methods. Alizarin red staining showed mineral deposition while transmission electron microscopy revealed a synthesis of extracellular matrix fibers during the differentiation process. Biochemical assays demonstrated that the differentiated phenotype expressed odontoblast markers, such as Dentin Matrix Protein 1 (DMP1) and Dentin Sialoprotein (DSP), as well as type I collagen. Quantitative data regarding the mRNA expression of DMP1, DSP and type I collagen were obtained by Real Time PCR. Immunofluorescence data demonstrated the various localizations of DSP and DMP1 during odontoblast differentiation. Based on our results, we obtained odontoblast-like cells which simulated the reparative dentin processes in order to better investigate the mechanism of odontoblast differentiation, and dentin extracellular matrix deposition and mineralization.
The Current View on the Use of Reconstruction Materials in Dentistry
Acta Polytechnica CTU Proceedings, 2017
The hardest tissue in the human body is the enamel which covers the anatomical crowns of teeth. It must be resistant to mechanical stress and the chemical attack of many substances from food, drinks and products of the metabolism of bacteria present in the oral cavity. These low pH substances dissolve the mineral components of enamel, cause tooth demineralization, and lead to decay or erosion damage with the irreversible loss of dental hard tissues and the necessity of their reconstruction. The range of dental materials intended for dental tissue reconstruction is extensive. Dental amalgam can be mechanically applied into the strongly stressed lateral segments of teeth. The use of amalgam is, however, in decline, with the possible health risks attributed to it, coupled with the need to extensively prepare tooth tissue promoting a shift towards using aesthetically and biologically favourable dental ceramic and polymeric materials instead. Current developments also concentrate on thes...
Induction of reparative dentin by calcium silicate-based material as direct pulp capping agent
Bangabandhu Sheikh Mujib Medical University Journal
This study was performed to examine whether calcium silicate could induce reparative dentin formation without eliciting any adverse effect in direct pulp capping of premolar teeth. Twenty participants who need extraction of their 4 healthy permanent premolar teeth for orthodontic reasons were included in this study. Following the surgical procedure, the exposed pulp tissue was treated either with calcium silicate or covered with calcium hydroxide paste. On day 3, 7, 14 and 28, the experimental teeth was extracted and examined using light microscopy and histometric analysis to observe the inflammatory changes and the amount of reparative dentin formation. The results showed that in the calcium silicate treated teeth, substantial amounts of dentine-like tissue was formed on day 14 and mostly located on the exposure site. It was also observed in the calcium hydroxide treated teeth but dentin-like tissue located at a distance from the exposure site. The total amount of reparative dentin...
Characterization of Bioactive Restoration/Dentine Interface
Egyptian Dental Journal
Objectives: To characterize the bonding interfaces of a calcium silicate-based restorative material (Biodentine) and CPP/ACP-modified glass ionomer with dentine. Also, this study was designed to evaluate the influence of dentine-surface treatment with poly acrylic acid on the bonding of both materials to dentine. Methods: Sixty caries-free human molars were used in this study. Specimens were randomly divided into three groups according to type of restorative material used (n=20); calcium silicatebased (Biodentine, Septodont, France), conventional GIC Fuji VII-EP, GC Corporation, Tokyo, Japan) or CPP/ACP-modified glass ionomer (Fuji VII-EP, GC Corporation, Tokyo, Japan). Each group was divided into 2 subgroups relative to the dentine surface treatment method (n=10); the dentine in control group was left without treatment, while the dentine surface in test groups was conditioned with poly acrylic acid (PAA) for 10s prior to the application of restorative. In each subgroup,5 specimens were double-labeled with fluorescein and rhodamine-B dyes then observed under a confocal laser scanning microscopy (CLSM). While the remaining 5 specimens were observed under scanning electron microscopy (SEM) then subjected to EDX elemental analysis. Results: CLSM micrographs showed that the mineral rich zone (MRZ) was obvious in nonconditioned Biodentine groups compared to conditioned groups. Both GICs groups exhibited obvious ion-exchange zones. In the non-conditioned Biodentine groups, polygonal crystal deposition was noticed beneath the hybrid layer. These findings were confirmed by the outcome of micromorphological analysis of restoration/tooth interface using SEM. Conclusions: Calcium silicate-based restorative material has a great affinity to exchange ions with tooth substrate and to bond chemically with dentine. The results of this study showed that calcium silicate-based restorative material (Biodentine) can be applied directly on dentine surface without any pre-surface treatment.