Measuring light-irradiance in cultures of phototrophic micro-organisms (original) (raw)

The Effect of Temperature on Photodiodes.pdf

In this work the effect of temperature and magnetic flux intensity as well on the output of three samples of photodiodes was studied . The current value in both light and darkness was calculated and found.

A Comparison Study of the Optical Measurement Sensitivity of the Phototransistor and Photodiode

Uluslararası Muhendislik Arastirma ve Gelistirme Dergisi, 2018

Depending on the amount of light they receive, the light-sensitive circuit elements generate electrical signals such as current, voltage, and resistance. Thus, these sensors are widely used in light measurement systems, light control systems, and opto-couplers. In this study underhand, the electrical values produced by the application of increasing light on the photodiodes and phototransistors are analysed. According to values obtained, the 9.44 standard deviation shows that photodiode has a better performance than phototransistor in terms of light sensitivity.

Measurement of light absorption and determination of the specific rate of light uptake in cultures of phototrophic microorganisms

Applied and Environmental Microbiology, 1996

This article describes a novel method for the measurement of light absorption by cultures of phototrophic microorganisms. The rate of light absorption is calculated as the difference between the rate of light output from a culture containing cells and the rate of light output from a culture containing only growth medium. The specific rate of light uptake is calculated by dividing the rate of light absorption by the total biomass present in the culture. Application of the method to several case studies shows that light output from a culture varies widely depending on the absorption and scattering characteristics of the suspension.

A model for light distribution and average solar irradiance inside outdoor tubular photobioreactors for the microalgal mass culture

Biotechnology and Bioengineering, 1997

A mathematical model to estimate the solar irradiance profile and average light intensity inside a tubular photobioreactor under outdoor conditions is proposed, requiring only geographic, geometric, and solar position parameters. First, the length of the path into the culture traveled by any direct or disperse ray of light was calculated as the function of three variables: day of year, solar hour, and geographic latitude. Then, the phenomenon of light attenuation by biomass was studied considering Lambert-Beer's law (only considering absorption) and the monodimensional model of Cornet et al. (1900) (considering absorption and scattering phenomena). Due to the existence of differential wavelength absorption, none of the literature models are useful for explaining light attenuation by the biomass. Therefore, an empirical hyperbolic expression is proposed. The equations to calculate light path length were substituted in the proposed hyperbolic expression, reproducing light intensity data obtained in the center of the loop tubes. The proposed model was also likely to estimate the irradiance accurately at any point inside the culture. Calculation of the local intensity was thus extended to the full culture volume in order to obtain the average irradiance, showing how the higher biomass productivities in a Phaeodactylum tricornutum UTEX 640 outdoor chemostat culture could be maintained by delaying light limitation.

A Simple Incubation Tank for Photosynthesis Measurements With Six Light Intensities

Annales de Limnologie-International …, 2009

The aim of the study was to design and put together a compact, easy-to-assemble and costefficient incubation system for aquatic plant photosynthesis measurements. Incubation tank consisting of glass sidewalls and mirror inner walls was constructed. The tank was split into six incubation cells and two water collecting cells. Each incubation cell was built of mirror with reflective side turned into the incubation cells to prevent the self shading of plants and minimized the variance of light intensity within the cell. The wall of each incubation cell facing the source of light was made of 3 mm glass and was covered by light absorbing film. To produce different light intensities single light source (fluorescent tubes) and light absorbing film (3M Scotchtint TM Sun Control RE50NEARL) were chosen. The sidewall of each incubation cell was covered with metal-coated neutral sun control film to produce six distinct light intensities. The different transparency of the sidewalls was achieved by lamination of the chosen film in increasing numbers of layers. The effect of the lamination on optical properties of the film was also studied. The variation of photon flux density within a cell was 3.9%. Continuous use of the system and occasional repetitive measurements of film’s transparency showed that it maintained its neutral optical properties over a long period of time.

A 5-Year Study of a New Kind of Photosynthetically Active Radiation Sensor¶*

Photochemistry and Photobiology, 2003

Light-emitting diodes (LED), which are designed as quasimonochromatic light sources, can also function as spectrally selective photodiodes. This provides a new kind of photosynthetically active radiation (PAR) sensor that is inexpensive and has much better stability over time than interference filters used in some PAR sensors. The action spectrum of photosynthesis in green plants has principle peaks in the blue and red regions. LED with response peaks in the UV-A (380 nm) and red (620 nm) regions have been used to measure PAR at or near solar noon in an ongoing study begun on 30 April 1996. The sum of the signals from the two LED is highly correlated with measurements by a calibrated filterless PAR sensor (Apogee QSO; Logan, Utah) from 13 September 1997 to 16 January 2002 (r 2 5 0.97). The sum of the LED signals is also highly correlated with measurements by a calibrated filter PAR sensor (LI-COR LI-190SA; Lincoln, Nebraska) from 20 April 1998 to 16 January 2002 (r 2 5 0.97). Thus, pairs of spectrally selective LED can function as PAR detectors in economical PAR radiometers. The separate 380 and 620 nm responses also permit an assessment of the differential impact of aerosol events on blue and red PAR and phototropic radiation.

Comparison of the sensitivities and accuracies of optoelectronic transducers for solar irradiance measurement

Sustainable Energy Research, 2023

This paper compares the sensitivities and accuracies of four different optoelectronic transducers in measuring solar irradiance for the fabrication of portable and low-cost pyranometers. Trans-impedance conditioning circuits were designed for two phototransistors (BP103 and SFH3310) and two photodiodes (BPW21 and BPW34). The Arduino Mega 2560 was used as an interface between the analogue signal produced by the conditioning circuits and the digital output ports. The transducers with a standard pyranometer RSRA_05V were arranged on a vero board and exposed to the sun. Statistical analysis of the experimental results produced the least root mean square error value of 6.58794 Wm −2 for phototransistor BP103 during the dry season followed by 13.35216 Wm −2 for phototransistor SFH3310 during the dry season. The correlation coefficients of BPW21, BPW34, BP103, and SFH3310 with the standard pyranometer are 0.9489, 0.9916, 0.9976, and 0.9905, respectively. The experimental results obtained from the phototransistors BP103 and SFH3310 strongly correlated with those of the standard pyranometer coupled with lower root mean square error and mean bias error values than those of the photodiodes. Thus, the phototransistors BP103 and SFH3310 are more accurate and effective for measuring solar irradiance. This study contributes to the development of low-cost and accurate solar radiation meters.