Chitosan–Collagen Electrospun Nanofibers Loaded with Curcumin as Wound-Healing Patches (original) (raw)
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This study focused on the development of biomedicated electrospun nanofiber mats for preventing wound infections and accelerating wound healing. Polycaprolactone (PCL) nanofiber-18 loaded curcumin (Cur) and polyethylene glycol (PEG) were generated by an electrospinning technique. The change in surface morphology of the electrospun nanofibers to porous surface 20 after immersion was obtained by field emission scanning electron microscopy (FE-SEM). The biological characteristics of the Cur-loaded PCL-PEG nanofiber mats such as cell viability, cell 22 attachment, anti-inflammatory and antibacterial properties, and in vivo wound healing capability were examined. The blending of PEG with PCL resulted in the formation of pores on the 24 nanofibers after immersion, which supports cell viability and proliferation. The mouse myoblast cell line C2C12 showed about 80% viability on the Cur-loaded PCL-PEG nanofiber mat. SEM 26 images showed that the cells could extremely attach and spread out over the surface of the Curloaded PCL-PEG nanofiber mat. The inclusion of 0.5 wt% Cur (with respect to PCL) in both the 28 PCL and PCL-PEG blended nanofiber mats inhibited excessive production of nitric oxide (NO) in RAW264.7 mouse macrophages and exhibited good antibacterial activity against 30
Curcumin nanofibers for the purpose of wound healing
Journal of Cellular Physiology, 2018
Poor wound healing is a highly prevalent clinical problem with, as yet, no entirely satisfactory solution. A new technique, termed electrospinning, may provide a solution to improve wound healing. Due to their large surface area to volume ratio and porosity, the nanofibers created by electrospinning are able to deliver sustained drug release and oxygen to the wound. Using different types of polymers with varying properties helps strengthening nanofiber and exudates absorption. The nanofibers appear to have an ideal structure applicable for wound healing and, in combination with curcumin, can blend the anti-inflammatory and antioxidant properties of curcumin into a highly effective wound dressing. The use of suitable curcumin solvents and the slow release of curcumin from the nanofiber help in overcoming the known limitations of curcumin, specifically its low stability and limited bioavailability. Here, we review the studies which have been done on synthesized nanofibers containing curcumin, produced by the electrospinning technique, for the purpose of wound healing.
Evaluation of curcumin-loaded chitosan nanoparticles for wound healing activity
ADMET and DMPK
Background and purpose: Wound healing is a biological process that can be difficult to manage clinically. In skin wound healing, the interaction of many cells, growth factors, and cytokines reveals an outstanding biological function mechanism. Wound healing that occurs naturally restores tissue integrity, however, it is usually restricted to wound repair. Curcumin synthesised in a chitosan matrix can be used to heal skin sores. Experimental approach: The ionotropic gelation procedure required crosslinking chitosan with a tripolyphosphate (TPP) crosslinker to generate curcumin nanoparticles encapsulated in chitosan. Key results: The nanoparticles were between 200 and 400 nm in size, with a strong positive surface charge and good entrapment efficacy, according to SEM and TEM investigations. Curcumin and chitosan compatibility was investigated using FTIR spectroscopy. All batches showed consistent drug release, with the F5 batch having the highest curcumin release, at 75% after 16 ho...
Characteristics of curcumin-loaded poly (lactic acid) nanofibers for wound healing
Curcumin (Cur) is a well-known extract of the root of Curcuma longa L. that has multi biological functions such as anti-oxidation, anti-inflammatory, anti-cancer , and wound healing properties. In the present study, poly (lactic acid) (PLA) nanofibers were used as a carrier for Cur because PLA nanofibers are biocompatible and have a high-specific surface area and high porosity, which can enhance the functional properties of Cur. The chemical and biological characteristics of Cur/PLA blended nanofi-bers containing varied amounts of Cur were examined. An increase from 0.125 to 6.250 wt% Cur in PLA caused a decrease in the diameters of the nanofibers from 971 ± 274 to 562 ± 177 nm. At Cur concentrations of \1.250 wt%, PLA and Cur showed good miscibility in the blended nanofibers, as shown by FTIR analysis and tensile tests. The inclusion of Cur in the blended nanofibers at concentration as low as 0.125 wt% promotes the attachment and proliferation of cells. The in vivo wound healing capability of Cur-loaded PLA nanofibers was assessed in a mouse model; treatment with Cur-loaded PLA nanofibers significantly increased the rate of wound closure (87 %) by day 7 compared with that of PLA nanofibers (58 %). The results of this study suggest that Cur-loaded nanofibers with appropriate Cur concentration are nontoxic and have potential as component of wound-healing patches.
Progress in Biomaterials, 2017
Active wound dressings play a significant role in burn and chronic wound treatment. In this study, electrospinning process is used to fabricate a novel three-layer active wound dressing based on e-polycaprolactone (PCL), polyvinylalcohol (PVA), and curcumin (CU) as a biologically active compound. The main purpose for developing such a system is to control wound exudates, which remains a challenge, as well as enjoying the anti-bacterial property. Electrospinning process parameters are optimized by response surface methodology to achieve appropriate nanofibrous electrospun mats, and then, a three-layer dressing has been designed in view of water absorbability, anti-bacterial, and biocompatibility characteristics of the final dressing. The results illustrate that a three-layer dressing based on PCL/curcumin containing PVA as a middle layer with optimized thickness which is placed over the incision, absorbs three times exudates in comparison with pristine dressing. Anti-bacterial tests reveal that the dressing containing 16% curcumin exhibits anti-bacterial activity without sacrificing the acceptable level of cell viability.
Electrospun curcumin/polycaprolactone/copolymer F‐108 fibers as a new therapy for wound healing
Journal of Applied Polymer Science, 2019
The application of fibers associated with drugs is a promising alternative to meet the clinical needs of tissue repair. Curcumin exhibits great cicatricial potential because it has numerous pharmacological properties. This research aimed to produce fibers of polycaprolactone and copolymer F-108 associated with curcumin and to evaluate in vivo their action on the process of wound healing. The fibers were produced by electrospinning technique and characterized by scanning electron microscopy (SEM), X-ray diffractometry (XRD), and fluorescence microscopy. They were applied in cutaneous wounds of rats for the analysis of photoacoustic permeation and histological study. The characterization showed that the electrospinning allowed the preparation of homogeneous material with curcumin. The fibers benefited healing of the wounds and allowed the permeation of curcumin at all stages. The use of PCL/F-108 fibers allowed the elaboration of a new curcumin delivery system, improving its bioavailability and action in the healing of excisional wound.
An Insight into Biofunctional Curcumin/Gelatin Nanofibers
Nanofibers - Synthesis, Properties and Applications, 2021
Electrospinning (ESPNG) was used to synthesize ultrathin (UT) and uniform nanofibers (from 5 nm to a few hundred nanometers) of various materials which have biomedical applications (BAs) such as dressing of wounds, drug discharge, and so on and so forth. In the first half of the report, there is an audit on the nanofibers having low diameter so that it could have larger surface area to volume proportion, likewise with that it would have sufficient porosity and improved mechanical properties required for wound healing. Nanofibrous mats (NMs) with high biocompatibility could be utilized during healing of wounds by sustained release of curcumin (Cc) and oxygen. The ESPNG was understood through in-depth numerical investigation in the present report. Furthermore, the process parameters (PMs) were reviewed in depth for their contributions in synthesizing UT - Curcumin/Gelatin (Cc/G) nanofibers (NFs) of optimum diameter. The aim of the discussion was to demonstrate that simply optimizing b...
Design of Curcumin-Loaded Electrospun Polyhydroxybutyrate Mat as a Wound Healing Material
Nano Biomedicine and Engineering, 2020
Nanotechnology and tissue engineering have accelerated wound healing. Polyhydroxyalkanoates, with suitable physical, biological and mechanical properties, can be considered as a good candidate in tissue repair and regeneration. In this study, nanofibrous mats of polyhydroxybutyrate (PHB) containing curcumin as a wound healing agent, were designed by electrospinning method. The samples were evaluated by microscopic and mechanical analyses, cell assays and microbial tests. The results of microscopic images showed that the diameter of fibers increased with the increase in the curcumin concentration. The elongation and elasticity modulus of nanofibers increased and decreased respectively, with the increase in the amount of curcumin. Drug release study indicated that increasing the curcumin concentration into nanofibers accelerated rate of drug release. Cytotoxicity results of nanofibrous samples with lower curcumin showed better biocompatibility. The strongest antibacterial activity was shown by the sample with 3% curcumin. In addition, Curcumin-loaded nanofibrous PHB can be potential candidates for wound healing.
Curcumin-loaded electrospun PHBV nanofibers as potential wound-dressing material
Journal of Drug Delivery Science and Technology, 2018
Advances in nanotechnology have led to the generation of many new wound-dressing materials with high surface area and high productivity at a low cost. The present study aims to fabricate curcumin-loaded nanofiber-based wound-dressing materials to enhance the bioavailability of curcumin and trigger the wound healing process. Curcumin (Cur) (0.1%, 0.3%, and 0.5% [w/v])-loaded poly(3-hydroxy butyric acid-co-3-hydroxy valeric acid) (PHBV) nanofibers were successfully produced via electrospinning. Morphological analysis revealed that the average fiber diameter of the nanofibers varied from 207±56 to 519±15 nm, depending on the curcumin concentration. The stiffness of nanofibers decreased with curcumin concentration. According to the release data, approximately 25% of the curcumin released in 30 min after the burst release. In addition, PHBV and PHBV/Cur nanofibers were not toxic to L929 mouse fibroblasts and enhanced the cell attachment and proliferation. Degradation studies, FTIR analysis, and swelling ratio measurements were also performed on nanofibers. Our results demonstrate that curcumin-loaded PHBV nanofibers can be used in wound-healing applications.
Journal of Cellular Biochemistry, 2019
Electrospun composite scaffolds show high ability to be used in regenerative medicine and drug delivery, due to the nanofibrous structure and high surface area to volume ratio. In this study, we used nanofibrous scaffolds fabricated by chitosan (CS), poly(vinyl alcohol) (PVA), carbopol, and polycaprolactone using a dual electrospinning technique while curcumin (Cur) incorporated inside of the CS/PVA fibers. Scaffolds were fully characterized via scanning electron microscopy, water contact angle, tensile measurement, hydration, protein adsorption, and wrinkled tests. Furthermore, viability of the buccal fat pad‐derived mesenchymal stem cells (BFP‐MSCs) was also investigated using MTT assay for up to 14 days while cultured on these scaffolds. Cell cycle assay was also performed to more detailed evaluation of the stem cells growth when grown on scaffolds (with and without Cur) compared with the culture plate. Results demonstrated that Cur loaded nanofibrous scaffold had more suitable c...