The Interaction between Apolipoprotein E and Alzheimer's Amyloid β-Peptide Is Dependent on β-Peptide Conformation (original) (raw)

The research investigates the interaction between apolipoprotein E (apoE) and amyloid β-peptide (Aβ) in the context of Alzheimer's disease (AD). It establishes that the binding affinity of Aβ peptides to human recombinant apoE3 and apoE4 is significantly influenced by the conformational state of the Aβ peptide, with a KD value of 20 nM observed for high β-sheet content Aβ peptides. The findings suggest that apoE preferentially binds to Aβ peptides with a β-sheet conformation, which could help explain the observed in vivo complexing of Aβ and apoE in senile plaques, emphasizing the role of Aβ conformation in AD pathogenesis.