Optimization of Production Technique and Nutritional Evaluation of Leaf Protein Concentrate from Tobacco (Nicotiana tabacum) (original) (raw)
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Journal of Food Science, 1980
Tobacco leaf proteins are well balanced, containing high lesels o f essential ;nino acids. This communication reports the content o f soluble leaf protein during different stages of development, and it s quality as predicted by chemical analysis and confirmed by biological evaluation. Results show tobacco leaf protein are at the top o f the plant protein range in nutritive quality and thus could provid e viable food by-products from the HLC process. Both 1. 1 protein an d soluble leaf proteins could be excellent supplements for cereal diet s consumed by world populations .
Journal of Medicinal Plants Studies, 2020
Protein is an important primary metabolite of our living systems. There are several studies based upon analysing the different, individual protein sources from various plant species are still on searching mode. On parallel with this context, traditionally edible plants has been proven to be an enriched source of protein based nutrition and largely utilized by most of the ethnic communities for their survival. The primary objective of the current research investigation is to estimate the total protein content from 33 traditionally used edible plant leaves categorized in trees, shrubs and herbs and to draw a comparative conclusion that which plant leaf contains better protein concentration. The protein content was estimated by UV-Vis spectrophotometric technique using the conventional Lowry's method. Bovine Serum Albumin (BSA) was used as standard reagent against which unknown protein concentration of plants had been estimated. All the proteins were extracted by using phosphate buffer (pH 7.4). From the results, it has been found that under tree category, Psidium guajava shows the highest (98.51 mg BSA Equivalent/ g of Fresh Weight) and Dillenia indica shows the lowest (13.73 mg BSAE/ g of FW) amount of protein content. In case of shrubs, Justicia adhatoda showed the maximum (86.37 mg BSAE/ g of FW) and Ocimum canum shows the minimum (10.59 mg BSAE/ g of FW) amount of protein content. Among the herbs, red Amaranthus viridis contains highest (97.43 BSAE/ g of FW) and Marsilea quadrifolia contains the lowest (15.04 mg BSAE/ g of FW) content of protein. The study results showed that Psidium guajava contains highest amount of protein among all the 33 plants. The study findings conclude that the protein content obtained from the leaves of different plant categories varies in their quantity and further supports the fact that plant leaves can be used as a potential source of nutrient consumption in near future.
The nutritive value of mixtures of white leaf protein and food proteins
Journal of the Science of Food and Agriculture, 1985
White leaf protein products were obtained from Medicago sativa by precipitation with isopropanol and from Chenopodiurn quinoa by heat fractionation and ultrafiltration/diafiltration. The isopropanol precipitated protein concentrate from Medicago contained less protein and more ash than the heat fractionated preparation from Chenopodiurn. The biological value of the protein fraction obtained from Medicago, estimated on rats using the Thomas-Mitchell balance method, was 59. The biological value of the protein preparation from Chenopodiurn was low (36). The preparations obtained were mixed with soya bean meal, wheat gluten, bone-meat meal, fish meal, skim milk and whey in a protein ratio 1:l. In every case, except fish meal, the biological value of the mixtures was higher than that of each component separately. This improvement of the nutritive value can not be explained from the amino acid composition; possibly the lysine availability was low. White leaf protein can, with advantage, supplement other food proteins.
Proceedings of the International Conference on Science and Education and Technology 2018 (ISET 2018), 2018
Tobacco is a plantation commodity used as the main ingredient of cigarettes. Nicotiana tabacum extract as biopesticides are nicotine content, d-limonene, pyridine, and indole on tobacco used as pest control of plants. This study examined the Effect of Nicotiana tabacum Extract Concentration as Biopesticide on protein content of Robusta coffee beans and skin. The research was conducted in March until August 2017 at Kebondalem, Jambu, Semarang with altitude of 711 meters above sea level. The study was designed a singlefactor of complete randomized block design. The concentration consisted of 0%, 0.1%, 0.2%, 0.3%, 0.4% named as (T0, T1, T2, T3 and T4) each treatment repeated 3 times. The results showed that the treatment without Nicotiana tabacum extract was very significant rather than with Nicotiana tabacum extract. The results of this research shows that the highest content of coffee beans protein was 0, 75% (T0), and the lowest was 0.47% T4) While the highest content of coffee skin protein content was 1, 46% (T0), and the lowest was 0.38% (T4).
2018
Leaf meal, protein and residues were produced from fresh leaves of Amaranthus spinosus, Amaranthus viridis, Telfairia occidentalis, Vernonia amygdalina, Bidens pilosa, Cnidoscolus aconitifolius, Manihot spp and Basella alba L and characterized for their proximate composition and some bio-active components. The leaf protein concentrate (LPC) in addition were characterized for their functional properties while three LPC with the highest crude protein (CP) content were analyzed for their amino acid profile and compared with FAO recommended pattern. Leaf meals (LM) contained on the average 16.25% ash (8.47–23.97%; CV%: 32.30), 24.96% crude protein (11.85 – 33.87%; CV%: 30.40), 9.94% crude fat (CF) (6.34 – 17.41%; CV%: 33.95) and 12.22% crude fibre (CFi) (5.73–18.45%; CV%: 32.33). In LPC products, the CP increased by 17.49-41.98%, CF by 11.82-35.73% while Cfi decreased by 24.38 – 83.71%. About 45 g of LPC from Manihot spp, 69g from T. occidentalis and 52 g of C. aconitifolius could be co...
Tobacco Seeds By-Product as Protein Source for Piglets
Open Journal of Veterinary Medicine, 2013
Tobacco seed cake is a by-product with interesting characteristics for animal nutrition, due to its high protein content. The focus of this study is to evaluate if tobacco seed cake, administered in feed, can affect principal serum metabolic parameters of weaned piglets in order to establish if it can be used as both a delivery system of edible vaccines and an alternative protein source in piglets diet. A total of 48 weaned piglets were divided in two homogeneous groups for weight, control (CG) and treatment (TG); TG and CG were fed ad libitum with two isoenergetic and isoproteic experimental diets (CP: 17.68% CG and 17.64% TG) differentiated for the inclusion of 4% of tobacco seed cake in the TG diet in replacement of wheat bran and soybean meal. Growth performances were evaluated and feed intake was measured weekly. Blood samples were collected on days 0, 20 and 43 to evaluate hematocrit and principal metabolic parameters, in order to assess the health status of the animals. The administration of tobacco did not impaire health status and growth performance of piglets. The use of bioenergy co-products from non-food crops may represent a good approach creating an integration between biofuel and food production, with consequent benefits for food security and environmental impact.
Nutritional Value of Commercial Protein-Rich Plant Products
Plant foods for human nutrition (Dordrecht, Netherlands), 2018
The goal of this work was to analyze nutritional value of various minimally processed commercial products of plant protein sources such as faba bean (Vicia faba), lupin (Lupinus angustifolius), rapeseed press cake (Brassica rapa/napus subsp. Oleifera), flaxseed (Linum usitatissimum), oil hemp seed (Cannabis sativa), buckwheat (Fagopyrum esculentum), and quinoa (Chenopodium quinoa). Basic composition and various nutritional components like amino acids, sugars, minerals, and dietary fiber were determined. Nearly all the samples studied could be considered as good sources of essential amino acids, minerals and dietary fiber. The highest content of crude protein (over 30 g/100 g DW) was found in faba bean, blue lupin and rapeseed press cake. The total amount of essential amino acids (EAA) ranged from 25.8 g/16 g N in oil hemp hulls to 41.5 g/16 g N in pearled quinoa. All the samples studied have a nutritionally favorable composition with significant health benefit potential. Processing ...
Critical parameters in cost-effective alkaline extraction for high protein yield from leaves
Biomass and Bioenergy, 2014
Leaves are potential resources for feed or food, but their applications are limited 6 due to a high proportion of insoluble protein and inefficient processing. To overcome these 7 problems, parameters of alkaline extraction were evaluated using green tea residue (GTR). 8 Protein extraction could be maximized to 95% of total protein, and, after precipitation by pH 9 adjustment to 3.5, 85% of extracted protein was recovered with a purity of 52%. Temperature, 10 NaOH amount, and extraction time are the protein yield determining parameters, while pH 11 and volume of extraction liquid are critical parameters for production cost. The cost of energy 12 and chemicals for producing 1t GTR proteins is minimized to 102€, and its nutritional value 13 is comparable to soybean protein. Furthermore, this technology was successfully applied to 14 other sources of biomass and has potential to be used as a part of an integrated bio-refinery 15 process. 16