Deterrent activity of plant lectins on cowpea weevil Callosobruchus maculatus (F.) oviposition (original) (raw)
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Insecticidal Activity of Plant Lectins and Potential Application in Crop Protection
Molecules, 2015
Lectins constitute a complex group of proteins found in different organisms. These proteins constitute an important field for research, as their structural diversity and affinity for several carbohydrates makes them suitable for numerous biological applications. This review addresses the classification and insecticidal activities of plant lectins, providing an overview of the applicability of these proteins in crop protection. The likely target sites in insect tissues, the mode of action of these proteins, as well as the use of lectins as biotechnological tools for pest control are also described. The use of initial bioassays employing artificial diets has led to the most recent advances in this field, such as plant breeding and the construction of fusion proteins, using lectins for targeting the delivery of toxins and to potentiate expected insecticide effects. Based on the data presented, we emphasize the contribution that plant lectins may make as tools for the development of integrated insect pest control strategies.
Lectins with Insecticidal and Insectistatic Activities
2018
Lectins are an important group of proteins which are spread in all kingdoms of life. Their most lighted characteristic is associated to their specific carbohydrate binding, although function has been not even identified. According to their carbohydrate specificity, several biological activities have been assessed, finding that lectins can be used as mitogenic agents, biomarkers, and cytotoxic and insecticide proteins. Lectins have been classified according to several features such as structure, source, and carbohydrate recognition. The Protein Research Group (PRG) has worked on Colombian seeds from the family of Fabaceae and Lamiaceae plants, isolating and characterizing their lectins, and found more than one lectin in some plants, indicating that according to its specificity, different lectins can have different biological activities. In the case of legume domain lectins, they have shown the biggest potential as insecticide or insectistatic agents due to the glycosylation pattern i...
Phytoparasitica, 2006
Lectin fromGlycine max L. was extracted and purified by affinity chromatography using asialofetuin-linked porous amino-activated silica beads. The concentration-dependent effect of lectin was studied on freshly laid eggs (0–8 h old) of the melon flyBactrocera cucurbitae (Coquillett); lectin failed to influence egg hatching. However, treating second instar larvae (64–72 h old) with increasing concentrations of lectin significantly reduced the development period, number of pupae and number of emergingB. cucurbitae, and was negatively correlated with the increase in the lectin concentration. The LC50 value, 54µg ml−1, was calculated on the basis of adult emergence. Treatment of the larvae (64–72 h old) with the LC50 concentration resulted in a decrease in pupal weight. The activity of three hydrolase enzymes (esterases, acid and alkaline phosphatases), one oxidoreductase (catalase) and one group transfer enzyme (glutathione S-transferase) was assayed in second instar larvae at the LC50 concentration of lectin after exposure for 24, 48 and 72 h. The activity of esterases increased significantly (P<0.01) at the three exposure intervals, whereas the activities of the three other hydrolyses and the transferases were significantly suppressed (P<0.01).
Plant Lectins with Insecticidal and Insectistatic Activities
Insecticides - Agriculture and Toxicology
Lectins are an important group of proteins which are spread in all kingdoms of life. Their most lighted characteristic is associated to their specific carbohydrate binding, although function has been not even identified. According to their carbohydrate specificity, several biological activities have been assessed, finding that lectins can be used as mitogenic agents, biomarkers, and cytotoxic and insecticide proteins. Lectins have been classified according to several features such as structure, source, and carbohydrate recognition. The Protein Research Group (PRG) has worked on Colombian seeds from the family of Fabaceae and Lamiaceae plants, isolating and characterizing their lectins, and found more than one lectin in some plants, indicating that according to its specificity, different lectins can have different biological activities. In the case of legume domain lectins, they have shown the biggest potential as insecticide or insectistatic agents due to the glycosylation pattern in insect midgut cells. This review attempts to identify the characteristics of plant legume lectin domains that determine their insecticidal and insectistatic activities.
Entomotoxic Potential of Plant Lectins as an Environment Friendly Tool to Control Insect Pests
Environmental Science Archives, 2023
A large number of insect pests infest crops at various stages including, pre-and post-harvest periods. Since immemorial times, agriculturists have applied numerous protective strategies to control insect infestation viz. variable cultural practices, crop rotation etc. and in modern times the application of chemical pesticides. Chemical pesticides impart environmental (soil, water) toxicity leading to health hazards and also have a negative impact on non-target species, thereby, disrupting natural biological control along with the development of resistance among target pests. Plant lectins combine specifically with the carbohydrate components of glycoproteins, glycolipids and other glycoconjugates in the pest and interfere with insect metabolism. Due to this property plant lectins can be utilised as defence proteins against phytophagous pests. Some of the plant lectins have been tested for their promising entomotoxic potential. This review demonstrates the entomotoxic potential of some candidate lectins and their impact on insect pests.
Natural Products: Plant Lectins as Important Tools in Controlling Pest Insects
Biorational Control of Arthropod Pests, 2009
Gal/GalNAc Ricin, SNA-I + Siaa2-6Gal/GalNAc *Insecticidal activity has been reported for many (+++), several (++), a few (+) or no (?) lectins from this plant lectin family. GNA, Galanthus nivalis agglutinin; ASAL, Allium sativum leaf agglutinin; ASAII, Allium sativum bulb agglutinin II; ACA, Allium cepa agglutinin; LOA, Listera ovata agglutinin; WGA, Triticum aestivum (wheat germ) agglutinin; UDA, Urtica dioica agglutinin; OSA, Oryza sativa agglutinin; BPA, Bauhinia purpurea agglutinin; ConA, Canavalia ensiformis agglutinin; PHA, Phaseolus vulgaris agglutinin; PSA, Pisum sativum agglutinin; GS-II, Griffonia simplicifolia agglutinin; SNA-I, Sambucus nigra agglutinin.
Entomologia experimentalis et applicata, 2001
Taro Colocasia esculenta (L. Schott) and rice (Oryza sativa L.) form a major part of the staple diet of pacific islanders. Pest constraints hamper the sustainability of taro and rice production in the Pacific region. Insect feeding trials were conducted in vitro to determine the effects of plant lectins against planthopper pests of taro and rice. Lectins were incorporated into artificial diet at 0.1% (w/v) level. The lectins Galanthus nivalis agglutinin (GNA) and concanavalin A (Con A) showed significant antimetabolic effects towards third instar nymphs of taro planthopper (Tarophagous proserpina Kirkaldy) whilst Pisum sativum agglutinin (PSA) showed no significant effects toward the insect. Psophocarpus tetragonolobus agglutinin (PTA) showed significant antimetabolic effects towards third instar nymphs of rice brown planthopper (Nilaparvata lugens Stål). PTA also reduced honeydew excretion levels of rice brown planthopper, over a 24-hour period, demonstrating antifeedant properties of the protein.
Plant lectins as defense proteins against phytophagous insects
Phytochemistry, 2011
One of the most important direct defense responses in plants against the attack by phytophagous insects is the production of insecticidal peptides or proteins. One particular class of entomotoxic proteins present in many plant species is the group of carbohydrate-binding proteins or lectins. During the last decade a lot of progress was made in the study of a few lectins that are expressed in response to herbivory by phytophagous insects and the insecticidal properties of plant lectins in general. This review gives an overview of lectins with high potential for the use in pest control strategies based on their activity towards pest insects. In addition, potential target sites for lectins inside the insect and the mode of action are discussed. In addition, the effect of plant lectins on non-target organisms such as beneficial insects as well as on human/animal consumers is discussed. It can be concluded that some insecticidal lectins are useful tools that can contribute to the development of integrated pest management strategies with minimal effect(s) on non-target organisms.