Protein Engineering of Industrial Enzymes (original) (raw)

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The paper discusses the advancements in protein engineering techniques applied to industrial enzymes, highlighting the significance of these enzymes in biocatalysis and their various applications in areas such as chemical production and laundry detergent formulations. It emphasizes the modifications made to enhance the stability, efficiency, and specificity of enzymes for commercial use, alongside the challenges faced in utilizing natural enzymes in industrial processes. Key examples include the engineering of subtilisin, a serine protease, to improve its performance under harsh conditions commonly present in detergent applications.

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Enzymes for industrial applications

OCL, 2017

Protéus is a biotechnology company specializing in the discovery, engineering and production of enzymes for industrial applications, as well as in the development of innovative bioprocesses involving these enzymes. Protéus is a subsidiary of the PCAS Group, actor in fine chemicals and specialty products and producer of high-value complex molecules. Enzymes allow considering unique functionalizations that are difficult to achieve by conventional chemical means. Examples involving the screening of our ready to use toolbox of lipases, the engineering of the well-known lipase CalB and the specific modifications of lipids will be presented.

A roadmap to directed enzyme evolution and screening systems for biotechnological applications

Biological Research, 2013

Enzymes have been long used in man-made biochemical processes, from brewing and fermentation to current industrial production of fi ne chemicals. The ever-growing demand for enzymes in increasingly specifi c applications requires tailoring naturally occurring enzymes to the non-natural conditions found in industrial processes. Relationships between enzyme sequence, structure and activity are far from understood, thus hindering the capacity to design tailored biocatalysts. In the fi eld of protein engineering, directed enzyme evolution is a powerful algorithm to generate and identify novel and improved enzymes through iterative rounds of mutagenesis and screening applying a specifi c evolutive pressure. In practice, critical checkpoints in directed evolution are: selection of the starting point, generation of the mutant library, development of the screening assay and analysis of the output of the screening campaign. Each step in directed evolution can be performed using conceptually and technically diff erent approaches, all having inherent advantages and challenges. In this article, we present and discuss in a general overview, challenges of designing and performing a directed enzyme evolution campaign, current advances in methods, as well as highlighting some examples of its applications in industrially relevant enzymes.

Molecular biology interventions for activity improvement and production of industrial enzymes

Bioresource Technology, 2020

Metagenomics and directed evolution technology have brought a revolution in search of novel enzymes from extreme environment and improvement of existing enzymes and tuning them towards certain desired properties. Using advanced tools of molecular biology i.e. next generation sequencing, site directed mutagenesis, fusion protein, surface display etc. now researchers can engineer enzymes for improved activity, stability, and substrate specificity to meet the industrial demand. Although many enzymatic processes have been developed up to industrial scale, still there is need to overcome limitations of maintaining activity during catalytic process. In this article recent developments in enzymes industrial applications and advancements in metabolic engineering approaches to improve enzymes efficacy and production are reviewed.

Industrial enzyme applications

Current Opinion in Biotechnology, 2002

The effective catalytic properties of enzymes have already promoted their introduction into several industrial products and processes. Recent developments in biotechnology, particularly in areas such as protein engineering and directed evolution, have provided important tools for the efficient development of new enzymes. This has resulted in the development of enzymes with improved properties for established technical applications and in the

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