Semen quality parameters in outbred male mice from four different selected lines (original) (raw)
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Sperm quality parameters are increased and asymmetric in house mouse hybrids
2019
Spermatogenesis is a tuned cascade of processes producing sperm; impairment of any phase of this process can affect fitness of males. The level of impairment can be pronounced in hybrids between genetically divergent populations. To explore the effect of hybridization on sperm quality we produced F1 hybrids from 29 wild derived strains of two house mouse subspecies, M. m. musculus and M. m. domesticus, which diverged 0.5 MY ago. The measured sperm quality traits did not significantly differ between intrasubspecific crosses. Effects of intersubspecific hybridization were dependent on sperm trait and cross direction. The proportion of sperm head abnormalities was increased in F1 intersubspecific hybrids. The frequency of dissociated sperm heads was increased in the M. m. musculus × M. m. domesticus (♀×♂) F1 but decreased in M. m. domesticus × M. m. musculus (♀×♂) F1 hybrids, with the difference in medians being more than 180%. We deduce that the dissociated sperm heads trait is associ...
PLoS ONE, 2014
Being subject to intense post-copulatory selection, sperm size is a principal determining component of male fitness. Although previous studies have presented comparative sperm size data at higher taxonomic levels, information on the evolution of sperm size within species is generally lacking. Here, we studied two house mouse subspecies, Mus musculus musculus and Mus musculus domesticus, which undergo incipient speciation. We measured four sperm dimensions from cauda epididymis smears of 28 wild-caught mice of both subspecies. As inbred mouse strains are frequently used as proxies for exploring evolutionary processes, we further studied four wild-derived inbred strains from each subspecies. The subspecies differed significantly in terms of sperm head length and midpiece length, and these differences were consistent for wild mice and wild-derived strains pooled over genomes. When the inbred strains were analyzed individually, however, their strain-specific values were in some cases significantly shifted from subspecies-specific values derived from wild mice. We conclude that: (1) the size of sperm components differ in the two house mouse subspecies studied, and that (2) wild-derived strains reflect this natural polymorphism, serving as a potential tool to identify the genetic variation driving these evolutionary processes. Nevertheless, we suggest that more strains should be used in future experiments to account for natural variation and to avoid confounding results due to reduced variability and/or founder effect in the individual strains. OPEN ACCESS Citation: Albrechtová J, Albrecht T, Ď ureje L, Pallazola VA, Piálek J (2014) Sperm Morphology in Two House Mouse Subspecies: Do Wild-Derived Strains and Wild Mice Tell the Same Story? PLoS ONE 9(12): e115669.
Initial characterization of an outbreed mouse model for male factor (in)fertility
Andrology, 2013
We analysed an outbreed mouse line which was selected for the phenotype 'high fertility' for 158 generations. During this selection period the mouse strain increased the number of offspring per litter from 10.4 to 17.1 and the total litter weight up to ~160%. In this study, we initially characterize the reproductive phenotype of high fertility males. Surprisingly, male bucks of the fertility line (FL1) show reduced percentage of motile and progressive motile spermatozoa; however, other sperm motility characteristics (e.g. velocity parameters) are improved compared with an unselected control line. Cytometrical investigation of the testicular cell-type composition indicated a significant increased concentration of diploid cells by a concomitant reduction in haploid cells in the testicular parenchyma of FL1. Furthermore, total testosterone concentrations in blood are dramatically increased in FL1 (>20 ng/mL). In line with increased testosterone levels, we observed increased ...
An evaluation of the mouse sperm morphology test and other sperm tests in nonhuman mammals
Mutation Research/Reviews in Genetic Toxicology, 1983
The authors are members of the Gene-Tox Work Group on Sperm Tests in Animals and Men. This paper was prepared as an interim report to evaluate the utility of a particular assay system. A.J. Wyrobek was the chairman of the reviewing committee. ** Operated by Union Carbide Corporation under contract W-7405-eng-26 with the U.S. Department of Energy. By acceptance of this article, the publisher or recipient acknowledges the right of the U.S. Government to retain a nonexclusive, royalty-free license in and to any copyright covering the article. Although the review described in this article has been funded wholly or in part by the U.S. Environmental Protection Agency through interagency agreement DOE 40-1123-80, EPA No. 80-D-X0953 to the Oak Ridge National Laboratory, it has not been subjected to the agency's required peer and policy review, and therefore, does not necessarily reflect the views of the agency and no official endorsement should be inferred. The protocols stated, suggested use of the assay in a screening program, and research recommended should not be taken to represent agency policy on these matters.
Animal Reproduction Science, 2006
A total of 2140 ejaculates from 156 adult males pertaining to four groups of bucks were analysed and the following traits were recorded: pH, ejaculate volume (V), mass motility (Mm), individual motility (Mi), concentration (Cn), total number of spermatozoa per ejaculate (TSE), percentage of sperm viability (Vi), percentage of sperm with acrosome integrity (NAR), percentage of sperm normalcy (Nr), percentage of sperm morphological abnormalities of head (H), neck-midpiece (Nm) and tail (T) and presence of proximal and distal cytoplasmic droplet (Dp, Dd). Principal component (PC) analysis and phenotypic correlations were performed in order to examine the relationships between qualitative and quantitative traits of rabbit semen. The repeatability of the traits measured was also estimated. Phenotypic correlations between sperm traits were estimated as the residual correlation from an analysis of variance, including the effects of: genetic type of the male, order of the ejaculate, day of collection and the permanent non-additive random effect of the male to which the observation corresponds. The repeatability of these traits was analysed separately, in a set of univariate analyses, using VCE software. The previously defined mixed model was used for this analysis. The principal component analysis was performed using the Princomp procedure of the SAS v.8 package. The first four PC explained 62% of total variation: 23%, 18%, 12% and 8%, respectively. Percentage of sperm viability, NAR, Nr, T and Nm were the predominant variables in the first PC. Mass motility,
Phenotyping male infertility in the mouse: how to get the most out of a 'non-performer
Human Reproduction Update, 2010
† Background Somatic cells in the testis Spermatogenesis The hormonal control of spermatogenesis Post-testicular sperm maturation Fertililization † Methods † Results Breeding experiments Histology Defects in spermiogenesis Sperm tail development and structure Hormone analysis Spermatogenic efficiency Analyzing post-testicular sperm maturation and fertilizing ability † Conclusion background: Functional male gametes are produced through complex processes that take place within the testis, epididymis and female reproductive tract. A breakdown at any of these phases can result in male infertility. The production of mutant mouse models often yields an unexpected male infertility phenotype. It is with this in mind that the current review has been written. The review aims to act as a guide to the 'non-reproductive biologist' to facilitate a systematic analysis of sterile or subfertile mice and to assist in extracting the maximum amount of information from each model. methods: This is a review of the original literature on defects in the processes that take a mouse spermatogonial stem cell through to a fully functional spermatozoon, which result in male infertility. Based on literature searches and personal experience, we have outlined a stepby-step strategy for the analysis of an infertile male mouse line. results: A wide range of methods can be used to define the phenotype of an infertile male mouse. These methods range from histological methods such as electron microscopy and immunohistochemistry, to hormone analyses and methods to assess sperm maturation status and functional competence.
BIOEDUKASI
Coexistence with the same sex is often discussed in recent times, but its effect on reproductive health has not been widely publicized. This study used male (Mus musculus) animals to determine the effect of co- rearing female animals on the quantity and quality of spermatozoa. The research type carried out was purely experimental with a completely randomized design with 4 treatments, namely control (P0) rearing which contained only 5 male mice, P1 rearing consisting of 3 males and 2 females, and P1 rearing consisting of 3 males and 2 females, P2 consisting of 2 males and 3 females while P3 contained 1 male and 4 female. Each treatment consisted of 6 replications. Animals are kept from 4 weeks old to 10 weeks old. Parameters observed are concentration, motility, and normal morphology of spermatozoa, and data analysis was tested by Kruskall-Wallis Test followed by Duncan’s test. the best spermatozoa compared to other treatments. The results showed that there were significant differenc...
International Journal of Andrology, 1984
Changes in the concentration, motility and morphology, of epididymal sperm have been evaluated in males of between 40 and 100 days of age in BALB, CBA, C3H and C57BL strains of mice. Particular attention was paid to sperm morphology, and a multiple entry classification system was used for its quantitation. At 40 days in each of the 4 strains studied sperm were few in numbers with poor motility and highly atypical morphology, particularly in the head region. From 80 days of age values for the 3 parameters were within the normal range. At equivalent ages during puberty the 4 strains differed in the concentration and motility of epididymal sperm, indicating difference in the time of onset of puberty between the strains. However, once adulthood was reached the strains differed only in the percentage of normal forms and in the frequency distribution of sperm with the various morphological abnormalities.