Comparison of Celisa and Blood Smears to Assess Anaplasma SPP Incidence in Dwarf Goats from Beoumi (Cote D?Ivoire) (original) (raw)

2018, International journal of advanced research

geographical areas. This makes the diagnosis difficult and their prevalence often underestimated (Ohashi et al., 2005). They are often the cause of several weeks of inactivity, sometimes leading to considerable losses in production and fertility on farms (Sharff, 2010; Moses and Phillip, 2013). Material and methods:-Study zone This study is a cross-sectional study. It was conducted from December 2015 to September 2016 on goats from the Béoumi sub-prefecture in central Côte d'Ivoire, in humid West Africa, between the tropic of cancer and the equator (FAO, 2005). This sub-prefecture covers an area of 1780 km 2 for 73475 inhabitants (INS, 2014). It is limited to the north by the sub-prefecture of Bodokro, to the south by the department of Sakassou, to the east by the department of Bouaké, to the west by the sub-prefectures of Kounahiri and Gohitafla (Fig. 1). Relief consists of a set of trays. Climate is of Baoulean type, characterized by a dry season and a wet season, with rainfall between 1200 and 1600 mm / year (FAO, 2005). Two rivers irrigate the sub-prefecture namely the Bandama River and the Kan River which is its tributary. This site was chosen because of the presence of a large number of goat farmers. Samples collection Study was conducted on a total of 270 dwarf goats coming from two different zones (Zone A and Zone B). These animals were divided into two groups. The first group including 135 dwarf goats were come from all the localities located at more than 15 km of the rivers (Zone A). This area is characterized by savannah which is covered with grass and shrubs. This herbaceous carpet is abundant during the rainy seasons but is destroyed by the fires in the dry season. The second group including also 135 goats were come from all the zones located close to the rivers Bandama and Kan between 0.5 and 1 km (zone B). These two rivers do not dry during the dry season; their bank promotes the development of vegetation likely to host some vectors of Anaplasma. Two types of sample were collected. The first type (270 blood sample) was taken out on the level of the jugular vein for the realization of the serological examinations (ELISA). The second type (270 blood samples) was taken out on the level of auricular vein for the parasitological examinations (blood Smears).