Identification and Characterization of Amentoflavone from Six Species of Juniperus Against H2O2 Induced Oxidative Damage in Human Erythrocytes and Leucocytes (original) (raw)
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A Study on the In Vitro Antioxidant Activity of Juniper ( Juniperus communis L.) Fruit Extracts
Analytical Letters, 2006
This study aimed at evaluating the in vitro antioxidant activity of water and ethanol extracts of juniper (Juniperus communis L., Family Cupressaceae) fruit. The antioxidant properties of both Juniper extracts were studied using different antioxidant assays, including reducing power, free radical scavenging, superoxide anion radical scavenging, hydrogen peroxide scavenging, and metal chelating activities. Both the water and the ethanol extracts exhibited strong total antioxidant activity. The concentrations of 20, 40, and 60 mg/mL of water and ethanol extracts of juniper fruit showed 75%, 88%, 93%, 73%, 84%, and 92% inhibition on peroxidation of linoleic acid emulsion, respectively. On the other hand, 60 mg/mL of standard antioxidant 47 such as butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), and a-tocopherol exhibited 96, 96, and 61 inhibitions on peroxidation of linoleic acid emulsion, respectively. However, both extracts of juniper had effective reducing power, free radical scavenging, superoxide anion radical scavenging, hydrogen peroxide scavenging, and metal chelating activities at these same concentrations (20, 40, and 60 mg/mL). Those various antioxidant activities were compared to BHA, BHT, and a-tocopherol as standard antioxidants. In addition, total phenolic compounds in both aqueous and ethanolic juniper extracts were determined as gallic acid equivalents. Accordingly, these results indicate that juniper has in vitro antioxidant properties and these may be major reasons for the inhibition of lipid peroxidation properties.
Free Radicals and Antioxidants, 2018
Background: Antioxidant plays a vital role in scavenging of free radicals, thus, providing protection against oxidative stress. Now the modern research is directed towards the discovery of herbal antioxidants and immnomodulators due to their lesser side effects. Objective: The aim of the present study is to evaluate the antioxidant and immunomodulatory potential of methanolic extract of Juniperus squamata from the Apharwat region of Gulmarg. Materials and method: The antioxidant activity of methanol extract of Juniperus squamata was evaluated by using 1, 1-diphenyl, 2-picrylhydrazyl (DPPH) scavenging, reducing power, hydroxyl radical scavenging, hydrogen peroxide scavenging activity and lipid peroxidation. Furthermore, the immunomodulatory potential of the extracts was investigated through the Delayed type hypersensitivity and phagocytic carbon clearance assay. Results: The highest phenolic content of 780 GAE/g (Total phenolic content) was observed in the methanolic extract, while the lowest Total phenolic content of 459 mg GAE/g was achieved in the petroleum ether extract. At a concentration of 700 g/mL, DPPH radical scavenging activity was found to be highest in methanolic extract (82.12%). Methanol extract was found to be an efficient scavenger of hydrogen peroxide radical and lipid peroxidation with IC 50 values of 161.44 ± 0.08 μg/mL and 78.65 μg/mL respectively. Administration of plant extract (100 and 200 mg/kg body weight) increased the DTH response and phagocytic carbon clearance significantly. Conclusion: The results indicate that the methanolic leaf extract of Juniperus squamata has good antioxidant and immunomodulatory potential.
Journal of the turkish chemical society, section a: chemistry, 2024
The purpose of the research was to determine Juniperus phoenicea L.'s elemental content, antioxidant activity, and phytochemical composition. Phytochemical screening was performed on four plant extracts (water, ethanol, chloroform, and ether). The aqueous and ethanol extracts were also analyzed for their total phenols, total flavonoids, and total antioxidant contents. The levels of macroelements (Na, Mg, Ca) and microelements (Fe, Cu, Zn) in the plant were determined by flame photometry and atomic absorption spectrometry. Moisture, ash, total protein, and total alkaloids were also determined. The results showed that the aqueous and ethanol extracts contained various phytochemicals, such as carbohydrates, proteins, phenols, tannins, flavonoids, alkaloids, coumarins, anthocyanins, saponins, and glycosides. The ethanol extract had higher concentrations of most phytochemicals than the aqueous extract, except for carbohydrates and proteins. The chloroform and ether extracts had lower concentrations of phytochemicals than the aqueous and ethanol extracts. The moisture, ash, total protein, and total alkaloid contents of the plant were 13%, 5.52%, 10.78%, and 1.84%, respectively. The total phenol contents, total flavonoid contents, and total antioxidant activity of the ethanol extract were 49.36±5.24 mg/g, 20.61±2.08 mg/g, and 34.82±2.44 mg/g, respectively. The corresponding values for the aqueous extract were 46.26±2.47 mg/g, 14.80±1.12 mg/g, and 37.32±3.29 mg/g, respectively. The order of abundance for macroelements was Ca (26860±950 mg/kg) > Na (1705.4±85 mg/kg) > Mg (944.4±38 mg/kg), whereas for microelements it was Fe (315.4±18 mg/kg) > Cu (55.52±3 mg/kg) > Zn (35.66±2 mg/kg). These results indicate that Juniperus phoenicea L. is a rich source of phytochemicals and elements that may have potential health benefits.
Czech Journal of Food Sciences, 2019
The content of phytochemicals, total phenolics, total flavonoids and antioxidant potential of extracts of Juniperus communis L. and Juniperus oxycedrus L. berries were determined. Ethanol, ethyl acetate and chloroform were used for the process of extraction. Phytochemical monitoring was based on already known methods, while in vitro antioxidant activities were done by DPPH assay. Phytochemical screening showed a wide spectrum of phytochemicals. Ethanolic extract of Juniperus communis L. possesses the strongest antioxidant activity (IC50 = 28.55 ± 0.24 µ/ml), as well the higher contents of total phenolics, 189.82 ± 0.27 mg of gallic acid equivalent per g of dried weight extract (mg GAE/g extract DW), and total flavonoids, 42.85 ± 0.13 mg of rutin equivalents per g of dried weight extract (mg RE/g extract DW). The results indicated the potential application of the tested extracts as significant antioxidants.
International journal of Pharmacy and Pharmaceutical Sciences, 2014
Objective: Today ' Methods: For evaluation antioxidant potentials, DPPH radical scavenging, determination of reducing power and phenolics were used. Gallic acid and quercetin were used as antioxidant standards. s attentions are focused on the finding new natural antioxidant compounds because of their fewer side effects than synthetic antioxidants. The aim of this study was to determine the antioxidant potentials of Juniperus excelsa fruit and its fractions by different methods. Results: The highest DPPH radical scavenging was observed in n-butanol fraction (IC 50 Conclusion: n-butanol fraction of Juniperus excelsa fruit had the highest radical scavenging, reducing power and phenolic compounds. In other words, a relationship between antioxidant potentials and phenolic compounds was found. Anyway, this fraction is a strong source of antioxidant compounds and can be used as a natural antioxidant. = 135.9±2.5 µg/ml) of Juniperus excelsa fruit. Also, this fraction possesses the highest reducing power (in 61.4±2.6 µg/ml with absorbance 0.5) and phenolic contents (82. 9±1.1 mg/g).
Industrial Crops and Products, 2015
This study investigates the phenolic compound content antioxidant and haemolytic activities in four extracts (methanol, water, hexane and dichloromethane) of Juniperus oxycedrus subsp. oxycedrus root bark. The methanol extract was the most concentrated in total phenolics (76.1 ± 2.8 mg GAE/g DW), flavonoids (39 ± 2.5 mg CE/g DW) and tannins (31.3 ± 2.1 mg CE/g DW). HPLC-MS n analysis of the methanol extract led to the identification of proanthocyanidin oligomers, quercetin hexose, quercitrin, and isorhamnetin hexose. The highest antioxidant activities were found in the methanol extract which exhibited the lowest IC 50 in all the antioxidant assays i.e. DPPH, ABTS, oxygen singlet, hydroxyl radical and superoxide anion scavenging assays, inhibition of the -carotene bleaching and lowest EC 50 in iron reducing assays. Incubation of the four extracts (20 mg/ml) with human erythrocytes for one hour led to haemolytic activities between 2.05% and 4.37%. The present findings suggest than the root bark could be used as food ingredient.
Molecules, 2019
In order to evaluate the antioxidant properties of aqueous and methanol extracts of needles and berries of Juniperus oxycedrus subsp. oxycedrus (Joo) species, various antioxidant capacity assessment tests (free radical scavenging assays (DPPH• and ABTS•+ tests), ferrous ions (Fe 2+) chelating activity and reducing power assay (FRAP) were conducted. In all of the tests, the extracts exhibited strong antioxidant activity. Furthermore, in-vitro cytotoxic activity assays of the methanolic extracts showed potent cytotoxic effects against two breast cancer cell lines (MDA-MB-468 and MCF-7), with no cytotoxicity towards normal cells (PBMCs). Reactive oxygen species generation was presumed to be a potential reason for the observed cytotoxic effects. According to all the above, and considering its appropriate composition of mineral elements and phenolic compounds, Joo could offer a beneficial and natural source of bioactive compounds that can be either used on the preventive side as it could potentially be used in the clinic without toxicity.
Antioxidant activities of Juniperus foetidissima essential oils against several oxidative systems
Revista Brasileira de Farmacognosia, 2011
The present study aimed to evaluate the antioxidant activity of essential oils obtained from branchlets of male and female trees as well as fruits of Juniperus foetidissima Willd., Cupressaceae, from Iran. For this purpose, essential oils of J. foetidissima were phytochemically analyzed and different concentrations of them were tested in five oxidative systems: 1) low-density lipoprotein oxidation; 2) linoleic acid peroxidation; 3) red blood cell hemolysis; 4) hemoglobin glycation; and 5) insulin glycation assays. In all employed systems, antioxidant effects were observed from the three tested oils though in varying degrees. The most promising activities of the oils were observed against hemoglobin and insulin glycation. Antioxidant activities of the oils did not appear to be dose-dependent. In addition, no consistent superiority in antioxidant effects was observed from a single oil in different assays. In view of the current results, J. foetidissima branchlet and fruit oils could be regarded as effective natural products with anti-glycation activity.
Juniperus sibirica Burgsdorf. as a novel source of antioxidant and anti-inflammatory agents
In order to examine antioxidant properties of methanol extracts of cones and needles of the unexplored Juniperus sibirica Burgsdorf. (Cupressaceae) species, various assays which measure free radical scavenging ability were carried out: DPPH, hydroxyl, superoxide anion and nitric oxide radical scavenger capacity test and reducing power (FRAP) assay. In all of the tests the extracts showed a potent antioxidant effect compared with BHT, a well-known synthetic antioxidant. In addition, the anti-inflammatory activity considering inhibitory potency toward COX-1 and 12-LOX was observed. Both extracts showed markedly anti-inflammatory activity, with needles having somewhat higher potency concerning both assays, reaching IC 50 at 1.29 mg/mL towards COX-1 and IC 50 at 1.34 mg/mL towards 12-LOX. Besides, in the extracts examined the total phenolic and flavonoid amounts were also determined, together with presence and content of the selected flavonoids: luteolin-7-O-glucoside, apigenin-7-O-glucoside, luteolin, apigenin, rutin and quercetin, which were studied using LC-MS/MS technique. LC-MS/MS analysis showed a noticeable content of natural products according to which the examined J. sibirica Burgsdorf. species could well be regarded as a promising new source of bioactive natural compounds, which can be used both as a food supplement and a remedy.
2014
the chemical composition of essential oils of juniper berries (Juniperus communis l.) were analyzed using GC/fID and GC/MS. Antioxidant properties were defined by 7 different in vitro models. the antioxidant activity attributable to electron transfer made juniper berry essential oil a strong antioxidant. IC 50 for hydroxyl radical (oH) scavenging and for chelating capacity were 0.0235 ig.(cm 3)-1 and 0.0246 ig.(cm 3)-1 respectively. the essential oil exhibited hydrogen peroxide scavenging activity and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (aBtS +) radical cation scavenging activity-the activity of 10 mg of juniper berry oil is equivalent to 4.77 mM trolox. the antioxidant activity of the oil attributable to hydrogen atom transfer was lower. IC 50 for 2,2-Diphenyl-1-picrylhydrazyl radical scavenging (DPPH) was found to be 944 ig.(cm 3)-1. lipid peroxidation inhibition by the essential oil in both stages, i.e. hydroperoxide formation and malondialdehyde formation, was less efficient than the inhibition by BHT. Through in vivo analyses with Saccharomyces cerevisiae yeast, the essential oil effect on the levels of the antioxidant enzymes SoD, catalase, and glutathione peroxidase was established.