Comparison of morpho-agronomic traits versus RAPD and ISSR markers in order to evaluate genetic diversity among Cuminum cyminum L. accessions (original) (raw)

2013, Australian Journal of Crop Science

Cuminum cyminum is a valuable medicinal plant belongs to family Apiaceae. In this study genetic variation among 42 cumin accessions were collected from different regions of Iran plus two accessions from Syria and Afghanistan were assessed based on three marker systems namely, ISSR, RAPD and morpho-agronomic traits. In overall, banding patterns of 22 ISSR primers and 13 RAPD primers revealed 202 (67.32%) and 85 (54.90%) polymorphic bands, respectively. The range of similarity coefficient in ISSR and RAPD markers were 0.48-0.92 and 0.25-0.94, respectively. Using primers as pairwise combination in this study did not offer higher polymorphism but provided different band pattern. Specific grouping were carried out by each cluster analysis including ISSR, RAPD, ISSR+RAPD and morpho-agronomic markers based on their similarity matrix making 8,7,6 and 3 groups respectively. The results showed that grouping based on molecular markers and morpho-agronomic traits are different so these two systems could not discriminate accessions as a same way. All of Mantel tests between extracted similarity matrices from each marker system were significant except between ISSR marker and morpho-agronomic traits. It could be concluded that among three different molecular data sets, the RAPD and RAPD+ISSR data have a significant and closer relationship to morpho-agronomic data.

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