In vitro evaluation of the suitability potential probiotic of lactobacilli isolates from the gastrointestinal tract of chicken (original) (raw)
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Pure and Applied Biology , 2019
Probiotic Lactobacilli are associated with normal microbial ecosystem of gastrointestinal tract (GT) of humans and chickens and plays an essential role in in-vivo interactions occurring in GT and hence exert health benefits beyond inherent basic nutrition. In recent study, some potential probiotic Lactobacillus bacteria were screened from chicken intestine. Twenty strains have been selected keeping in notice their growth in Lactobacillus selective media i.e. De Man, Rogosa and Sharpe agar (MRS). They were subjected to gram staining, catalase activity and oxidase test. Out of these, 5 strains have been selected for detailed studies on the basis of best antimicrobial activity and Lactobacillus specific biochemical tests. These five strains were named as (LS1, LS2, LS3, LS4 and LS5) and their probiotic potential has been evaluated. Out of these five strains, LS4 has shown maximum antimicrobial activity (25.10 ± 2.008) by producing antimicrobial bacteriocins like compounds, which have inhibitory property against potential pathogenic strains. All the strains were stable in both acidic and alkaline pH. These strains were also tolerant to different concentrations of bile salts and sodium chloride (NaCl). Their temperature tolerance was also evaluated and observed that is an important selection criteria for probiotics. This study is extremely promising that underscores the important role of Lactobacillus strains, having probiotic effects, which may play an important role in food industry as starter-culture, co-culture and bio-protective cultures to standardize and enhance quality and safety of preserved food and beverages.
Selection of lactobacilli for chicken probiotic adjuncts
Journal of Applied Microbiology, 1998
M . G AR R IG A, M . P AS C UA L, J .M . M O NF OR T AN D M . HU GA S . 1998. During inhibitory activity screening of 296 strains of lactic acid bacteria from the gastro-intestinal tract of chicks, 77 strains showed inhibition against enteric indicator strains (Salmonella enteritidis and Escherichia coli)
Lactobacilli Isolated from Chicken Intestines: Potential Use as Probiotics
Journal of Food Protection, 1999
Lactobacillus strains were tested for their in vitro probiotic properties. Cell surface hydrophobicity was found to be very high for Lactobacillus fermentum subsp. cellobiosus and Salmonella Gallinarum; high values could indicate a greater ability to adhere to epithelial cells. Studies on Lactobacillus animalis indicated relative cell surface hydrophobicities smaller than those of L. fermentum subsp. cellobiosus and L. fermentum. L. animalis and Enterococcus faecalis were able to coaggregate with L. fermentum subsp. cellobiosus and L. fermentum, respectively, but not with Salmonella Gallinarum. After mixed-culture studies for determining suitable growth behavior, the pair of strains L. animalis plus L. fermentum subsp. cellobiosus was selected for an attempted challenge against Salmonella Gallinarum. Double and triple mixed-culture studies indicated that selected lactobacillus strains were able to retain their beneficial characteristics in the presence of Salmonella Gallinarum such ...
Probiotic Properties of Lactobacillus Isolates from Chicken Intestines
2019
1Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia. 2Agro-Biotechnology Institute Malaysia (ABI), c/o MARDI Headquarters, 43400 Serdang, Selangor, Malaysia. 3Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia. 4School of Bioscience and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia.
Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 2014
Lactobacillus spp. isolated from different portions of chickens' gastrointestinal tract were evaluated concerning their ability to survive in a water-in-oil (W/0) emulsion containing sesame and sunflower oil. After sixty days of emulsion storage under refrigeration, three of five strains tested survived in number equal to or higher than 10(6)cfu/g. Lactobacillus reuteri 2M14C, which presented the highest survival in W/O emulsion (10(7)cfu/g), was tested for its capacity to resist throughout the passage through gnotobiotic mice gastrointestinal tract and for the ability to stimulate murine peritoneal macrophages phagocytosis. This strain remained at a number above 10(9)cfu/g feces during ten days of monoassociation, and monoassociated mice showed phagocytic activity significantly greater than the germ-free controls (P<0.05). The results suggest that the formulation can be used to incorporate viable Lactobacillus spp. cells in animal feed. Moreover, the results suggest that L. ...
Polish journal of microbiology / Polskie Towarzystwo Mikrobiologów = The Polish Society of Microbiologists, 2005
Lactobacilli were isolated from chicken gastrointestinal tract and examined for their potentially probiotic properties towards their inhibitory activity against poultry enteropathogenic bacteria. Biochemical tests, ITS-PCR and cell wall protein analysis were used to characterize the Lactobacillus isolates. The identification of isolated Lactobacillus strains based on phenotypic properties was not always satisfactory. ITS-PCR together with protein profile were found to be helpful in strain identification. Lactobacilli were tested for the inhibitory activity against selected strains of poultry enteropathogenic bacteria (Salmonella Enteritidis, Escherichia coli and Clostridium perfringens). Examined supernatants from Lactobacillus broth cultures demonstrated major antimicrobial activity against C. perfringens. Lower antimicrobial activity were observed against E. coli and Salmonella Enteritidis. The strongest inhibition effect were obtained using supernatant of Lactobacillus acidophilu...
A total of 113 lactic acid bacteria (LAB) were isolated from crop, gizzard, intestine and ceca regions of broiler chicken. Thirteen Lactobacillus isolates were selected based on their ability to grow on acidic pH and inhibition of enteric bacterial pathogens. They were identified using 16S rRNA and recA gene sequencing and evaluated for probiotic properties in vitro. These isolates showed effective inhibition against enteric bacterial pathogens like Escherichia coli and fungal pathogens, such as Aspergillus niger, A. flavus, Penicillium expansum, P. roqueforti, Candida albicans and Eurotium species. Analysis of 16S rRNA and recA gene sequences revealed that isolates belonged to Lactobacillus plantarum. They exhibited tolerance to bile salts (0.4%), gastric and intestinal conditions with bile salt hydrolase activity, suggesting their ability to survive during gastrointestinal transit and exert their probiotic action on host organism. They showed cell surface hydrophobicity (39–58%), auto-aggregation (60–80%) and co-aggregation with enteric pathogens like E. coli (49–62%) and Listeria monocytogenes (26–38%). Thus, they could potentially be bactericidal to pathogens and prevent their colonization on intestinal epithelium. They are non-pathogenic (γ-hemolytic) and negative for mucin degradation. Among these isolates, L. plantarum VJI21 and VJC1 showed more adherence to HT-29 cells than the enteric pathogen E. coli and prevented pathogen adherence to intestinal cells. L. plantarum VJI21 and VJC1, having potent probiotic properties, are considered good candidates for further studies in vivo towards the development of probiotic feed supplement in the poultry industry.
International Journal of Biotechnology for Wellness Industries, 2016
This study was aimed at selecting novel strains of Lactobacillus from crop of Algerian poultry. One hundred forty (140) lactobacilli strains were isolated and examined for their potentiality probiotic properties. From these isolated strains, nine appear to possess a probiotic value and highlighted a noticeable heterogeneity. The isolate L. plantarum G1 showed the best inhibitory activity against several indicator strains. Furthermore, the results showed that culture and neutralized supernatants exhibited varying degrees of inhibitory activity against strains of enterobacteria from poultry origin. The tested strains were acid resistant and were also bile tolerant. Antibiotic resistance, co-aggregation activity and hydrophobicity percentage were strain-dependent. Moreover, six strains were able to adhere to epithelial cells. Finally, six Lactobacillus strains, such as strain L. plantarum G1, L. plantarum PC2, L. viridesencs G3, L. helveticus PC6, L. delbrueckii ssp delbrueckii G7 and L. fermentum PC8, showed essential probiotic properties. The identity of the best strain L. plantarum G1 was confirmed by 16S rRNA gene sequence using PCR.
Identification and probiotics properties of Lactobacillus strains from poultry crop
World Poultry Science Association 2nd Mediterranean Summit of Wpsa Antalya Turkey 4 7 October 2009, 2009
Sixteen lactobacilli strains were isolated and identified from poultry crop and examined for their potentiality probiotic properties. Results showed that the strain tested were resistant to pH3, resistant to a number of antibiotics and were also bile tolerant. During inhibitory activity screening, Lactobacillus bifermentans PC 1 showed the best inhibition of a range of indicator strains tested. Culture supernatants and neutral supernatants exhibited varying degrees of inhibitory activity against strains of enterobacteria.
Polish journal of veterinary sciences
The probiotic potential of a Lactobacillus salivarius 3d strain isolated from chicken faeces was assessed in one day old chickens. Lactobacillus salivarius 3d was administered per os at a concentration of 10(8) cfu in 100 microl of PBS. The chickens were then challenged with pathogenic bacteria: Salmonella Enteritidis, Campylobacter jejuni and Clostridium perfringens. Samples of caecal contents and livers were collected after 1, 2, 3, 7 and 14 days after infection. Lactobacilli and pathogenic bacterial cell counts were determined in the samples. This study showed that L. salivarius 3d reduced the number of Salmonella Enteritidis and Clostridium perfringens in the group of chickens treated with Lactobacillus. Therefore it may be concluded that L. salivarius 3d may be used as a potential probiotic for chickens.