Is there Channeling of Intermediates in the Chloroplast? (original) (raw)

1987

Abstract

In experiments designed to examine the relationship between light activation of reductive pentose phosphate cycle enzymes and induction we were surprised to find that the active site concentrations of NADP-1inked glyceraldehyde-3-P dehydrogenase and aldolase exceed substrate concentrations. The potential activity of these enzymes is then not sufficient to support observed rates of photosynthetic CO2 fixation. Since photosynthetic CO2 fixation does occur either our estimates are in error or some mechanism exists for channeling metabolites between enzymes in the chloroplast. Experiments with broken chloroplast extracts are consistent with transfer of P2-glycerate between P-glycerate kinase and glyceraldehyde-3-P dehydrogenase without diffusion into the surrounding medium.

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