Isolation and Characterization of Carbohydrates (original) (raw)

Starch from potato and glycogen from chicken liver were isolated for the general test for polysaccharides, which are the molisch's test and the iodine reaction test, hydrolysis of polysaccharides (acidic and enzymatic) and for the tests such as Benedict's, Barfoed's, Seliwanoff's, and Bial's Orcinol Tests. Quantitative analysis was performed with glucose solution employing absorption spectrometry which determined the concentration of the given unknown sample using the equation of the line obtained from the graph. The absorbance of the unknown solution was 0.620 resulting to the quantifying its concentration which was 0.000896 M.