DREAM binds to the p21 promoter (original) (raw)

2011

Abstract

<b>Copyright information:</b>Taken from "The repressor DREAM acts as a transcriptional activator on Vitamin D and retinoic acid response elements"Nucleic Acids Research 2005;33(7):2269-2279.Published online 22 Apr 2005PMCID:PMC1084319.© The Author 2005. Published by Oxford University Press. All rights reserved () Schematic representation of the 5′-flanking region of the p21 gene showing the location of the primers used to amplify the fragments that contain the VDRE and the RARE used in the ChIP assays. The assays were performed in untreated HL-60 cells and in cells treated with vitamin D or RA for 2 h. Representative assays for both regions performed with control and anti-DREAM antibodies are shown in the lower panels. The inputs of the assays for untreated and treated cells are shown in lanes 1 and 2, respectively. () The upper panel shows the position of the RARE of the RARβ2 promoter and the region amplified in the ChIP assays in control and RA-treated cells is shown in the lower panel. In (), ChIP assays were performed with an antibody against acetylated histone H4 (αAcH4). In the left panel are shown the results obtained with the regions amplifying the RARE and the VDRE of the p21 gene in cells treated with RA and vitamin D, respectively. The right panel illustrates the amplification of a GAPDH promoter fragment, used as a negative control, in control and vitamin-D treated cells. () Recruitment of the p160 coactivator SRC-1 to the RARE and VDRE regions of the p21 promoter in control cells and after treatment with RA or vitamin D, respectively. In all panels, relative recruitment in untreated versus ligand-treated cells was quantified and is shown as fold induction.

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