Brief Report: Graft-Versus-Host Reaction in F1 Hybrid Mice Injected with Pre-Immunized Parental Thymus Cells (original) (raw)
Related papers
Evidence for a small pool of immunocompetent cells in the mouse thymus
Experimental Cell Research, 1969
The graft-versus-host reactivity of thymus cells from normal mice was compared with the reactivity of thymus cells from mice previously injected with cortisone or X-irradiated with 400 R. The reactivity of the cortisone-resistant cells was ten times higher than the reactivity of normal thymus cells, whereas X-irradiation did not increase the relative reactivity of the thymus cells. The cortisone-resistant cells, which are known to be located in the thymus medulla, were found to have the same volume distribution as normal lymph node lymphocytes.
The American journal of anatomy, 1984
A small but definite proportion of T-lymphocyte-like cells have been reported in nu/nu (nude) mouse spleen despite the congenital absence of a thymus in these animals. We have determined the number and the characteristics of such cells using flow cytometry. The level of T-like cells increased with age. In 4-month-old nu/nu CBA spleen, 14% of all cells expressed some Thy 1 antigen. However, only 4% expressed mature T-cell levels, and only the 2% with the highest Thy 1 also showed a normal distribution of Ly 1 and Ly 2 antigens. These T-like cells were slightly larger than normal nondividing T lymphocytes. We have assessed the total functional capacity of T-like cells in nu/nu CBA spleen using a high-cloning-efficiency limit-dilution culture system. Almost all precursor cells capable of forming clones when stimulated with concanavalin A in the presence of irradiated spleen cells and growth factors, and almost all precursors of those clones that were cytolytic in a lectin-mediated tumo...
Proliferation and cytotoxicity of thymus lymphocytes in vitro
Bulletin of Experimental Biology and Medicine, 1977
The proliferative and cytolytic activity of lymphocytes from the spleen and intact thymus was compared after alloimmunization. The number of living cells and of DNA-synthesizing cells in a monoculture of thymocytes was 90-97% less, and in a mixed culture of thymus cells about 80% less than the corresponding number of spleen cells. The index of stimulation of immune thymocytes was several times greater than that of immune spleen cells. The peak of cytotoxicity was observed on the fourth to fifth day of stimulation, when the cytolytic activity of the immune thymocytes was close to the activity of immune spleen cells. The low DNA synthesis and the considerable cytotoxic activity of the immune thymocytes mean that stimulation of thymus cells in vitro can be used to obtain a cell population with a high content of cytolytic T lymphocytes.
Journal of Experimental Medicine, 1970
By means of an assay of graft-versus-host activity some properties of the thymic humoral factor which confers immunocompetence upon lymphoid cells in vitro have been studied. Allogeneic and xenogeneic thymic preparations were found to activate lymphoid cells from neonatally thymectomized mice, enabling initiation of a graft-versus-host response. Thus, this thymus factor is apparently neither strain nor species specific. The active principle of calf thymus extracts was found to be in the supernatant after prolonged ultracentrifugation. When exhaustive dialysis and ultrafiltration through Diaflo membranes were performed, the active thymus agent was found to pass through both the dialysis sac and Diaflo UM-2 membranes. The molecule which confers immunocompetence upon lymphoid cells thus seems to be of molecular weight of an order of magnitude of 1000 or less. Dialyzed thymus preparations injected into neonatally thymectomized mice also restored the capacity of spleen cells of these mic...
Development of T cells in SCID mice grafted with fetal thymus from AKR mice or F344 rats
European Journal of Immunology, 1993
To examine the development of Tcells within an allogeneic or xenogeneic environment, we engrafted the fetal thymus from AKR mice or F344 rats under the kidney capsule of SCID mice (mTG and rTG mice). Tlymphopoiesis developed in SCID mice 2 months after transplantation, although the ratio of CD4/CD8 in both experimental groups was different from that of normal control. T cells in mTG mice did not show in vim proliferation or cytotoxicity against either host-type C.B-17 (H-2d) or donor-type AKR (H-2k) cells, while they exerted potent activities against third-party B10 (H-2b) cells. In contrast, T cells in rTG mice exhibited proliferation against both host-type C.B-17 and donor-type F344 rat cells. Consistently, graft-vs. -host disease symptoms developed in these mice and histological examination showed impressive infiltration of lymphocytes into the skin or into the mucosal layers of the stomach. Activated state of T cells in rTG mice was also evidenced by the positive expression of interleukin-2 receptor. Taken together, fetal thymus appears to contain progenitor cells which are sufficient for in vivo reconstitution of T lymphopoiesis, but species-specific environment is important for the induction of tolerance. In mTG mice, Vp6+ T cells reactive to donor Mls" determinants and Vf33+ T cells reactive to host MlsC determinants were deleted, suggesting that tolerance was regulated mainly by clonal deletion. By contrast,Vpll+ T cells reactive to Mlsf determinants were not deleted possibly due to the lack of their ligands.