Differential IFN‐γ production by adult and neonatal blood CD56+ natural killer (NK) and NK‐like‐T cells in response to Trypanosoma cruzi and IL‐15 (original) (raw)

2013, Parasite Immunology

SummaryEarly interferon‐gamma (IFN‐γ) release by innate cells is critical to direct type 1 immune response able to control intracellular pathogens like Trypanosoma cruzi. Although CD56bright natural killer (NK) cells are reported to be potent early IFN‐γ producers, other CD56+ cells like CD56dim NK cells and NK‐like T cells have recently been shown to also release IFN‐γ. We have here studied the contribution of each CD56+ lymphocyte populations in early IFN‐γ production in both adults and neonates. On this purpose, we analysed the kinetics of IFN‐γ production by RT‐PCR, ELISA and flow cytometry from 2 h onwards after T. cruzi and IL‐15 stimulation and sought for the responding CD56+ cells. CD56bright and CD56dimCD16− NK cells were the more potent IFN‐γ early producers in response to IL‐15 and parasites in adults and neonates. In both age groups, the majority of IFN‐γ producing cells were NK cells. However, on the contrary to neonates, CD3+CD56+ NK‐like T cells and CD3+CD56− ‘classic...

Sign up for access to the world's latest research.

checkGet notified about relevant papers

checkSave papers to use in your research

checkJoin the discussion with peers

checkTrack your impact