Genetic diversity of Cucurbita moschata inbred lines selected from six different populations using HFO-TAG markers (original) (raw)
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Genetic divergence in pumpkin (Cucurbita moschata L.) Genotypes.pdf
Genetic diversity using Mahalanobis's D 2 technique was studied for yield and its components on twenty one genotypes of pumpkin (Cucurbita moschata L.). Quantification of variability for each character was done using the Shannon Weaver Diversity Index. High degree of variation was exhibited within the collection, as reflected by mean diversity index value of 0.80. Data were subjected to principal component analysis (PCA), principal coordinate analysis (PCO), canonical variate analysis (CVA) and non-hierarchical clustering to identify suitable parents having distant relationship for hybridization program. The genotypes were grouped into five different clusters. Cluster IV contained the maximum number of seven genotypes whereas cluster I contained least number having only one genotype. The lowest inter-genotypic distance (0.75) was found between BD-2174 and BD-9489 where the highest (47.46) was between BARI Mistikumra-1 and BD-2150. The maximum inter cluster distance was observed between cluster II and III (17.922) and the minimum inter cluster distance was observed between cluster II and IV (6.825). The maximum intra cluster distance was noticed for the cluster V (0.261) and the minimum intra cluster distance was found in cluster I (0.00). Cluster I contained the highest mean values for pedicel length of male flower, number of male flowers/plant, fruit length, fruit breadth, single fruit weight and fruits/plant. Cluster II contained the highest mean values for days to first male and female flowering. Cluster III contained the highest mean values for leaf breadth, pedicel length of female flower and number of female flowers/plant. Leaf breadth, pedicel length of male flower, number of male flowers/plant and fruits/plant were the important components of genetic divergence in the studied materials. Based on inter cluster distance, inter genotypic distance and consideration of desirable characters for high yield potential, the genotypes G19 (BARI mistikumra-1) and G20 (BARI mistikumra-2) from cluster II; G21 (BD-2150) from cluster I and G1 (BD-2151) and G13 (BD-266) from cluster III can be selected as better parents for future hybridization program.
Bulletin of the National Research Centre
Background Cucurbita spp. is a main source of crypto-xanthine, zeaxanthin lutein folates, and natural poly-phenolic flavonoid compounds. Collection and conservation of genetic variability are helpful in genetic advancement programs. Twenty-two pumpkin genotypes (21genotypes of Cucurbita pepo L. and one genotype of C. maxima L.) were collected from different regions of Saudi Arabia. Fifteen HFO-TAGhigh frequency oligonucleotide–targeting active gene markers were used to analyze genetic variability among 22 pumpkin genotypes. Results A total of 107 alleles were detected by the 15 HFO-TAG markers, an average of 7.133 alleles per primer. Polymorphisms were found in 102 alleles, an average of 6.866 alleles per primer. The PIC values measured from all of the HFO-TAG markers were high, and ranged from 0.8940 to 0.7225, with an average 0.8212 per marker. Conclusions The results of the cluster analysis of pumpkin genotypes were separated into seven groups according to the collection region.
Genetic Divergence in Pumpkin (Cucurbita Moschata Duch Ex Poir)
A Field experiment was conducted in Research and Development farm of Lal Teer Seed Ltd. to estimate the genetic divergence among the 21 genotypes of pumpkin were estimated using D2 and principal component analyses. The genotypes under study grouped in to six clusters. The inter-cluster distance was larger than the intra-cluster distance suggesting wider genetic diversity among the genotypes of different groups. The intra-cluster value was maximum in cluster VI and minimum in cluster III. The maximum inter cluster distance average D2 value was observed between cluster I and cluster III (25.75) indicating wide range of genetic diversity between these two clusters. Thus the genetically diverged genotypes could be used as parent in hybridization program for getting desirable segregants. The lowest inter cluster divergence was observed between cluster III and IV (7.14). Cluster I showed the lowest mean values for days to first male and female flowering and highest mean values for flesh thickness, number of seeds per fruit and brix (%), while cluster VI revealed the highest mean vales for fruit diameter, single fruit weight and yield per plant. Germplasms much in use of the above mentioned characters both in cluster I and VI would offer a good scope of improvement of the crop through rational selection for hybrid breeding program.
Biological forum, 2023
Genetic variability and its proper characterization are of vital importance in hybridisation programme of a crop for making a successful rapid improvement. In India, there is a wide range of genetic variability in pumpkin but, not much attention has been given to its improvement programme. Moreover, pumpkins (Cucurbita spp.) are grown as a summer crop in Terai region of West Bengal and no suitable genotypes are available for winter season cultivation. Hence, the present study focuses on identifying and conserving the superior genotypes for off-season to facilitate the future breeding programme. The present experiment was carried out at the experimental field of the department, Vegetable and Spice Crops,
Genetic divergence among pumpkin (Cucurbita moschata Duch ex. Poir) landraces of Assam
The Pharma Journal , 2022
Pumpkin (Cucurbita moschata Duch.) is one of the most popular summer vegetable crops and belongs to the Cucurbitaceae family. Thirty diverse pumpkin landraces representing eight districts of Assam comprised the investigation, carried out at the Instruction-cum-Research (ICR) Farm of Assam Agricultural University, Jorhat, during Rabi 2018-2019. Genetic divergence in 30 pumpkin landraces for 27 different traits using Mahalanobis D 2 statistics and Tocher's clustering generated five clusters of the 30 pumpkin landraces. Diversity analysis revealed that the highest genetic diversity was evident between the cluster pairs II-V, I-V, I-V and IV-V, respectively; the landraces from these clusters may be selected as parents in future hybridization programs to obtain superior combinations and heterosis in segregating generation. The single landrace Dibrugarh-1 belonging to cluster IV stood most diverse among 30 landraces and recorded a high cluster mean for yield per plant, which can serve as a suitable parent for the hybridization programme. Landraces Karbi Anglong-1, Haflong-1, Jorhat-1, Majuli-1 and Lakhimpur-1 belonged to the best mean cluster group V; they showed the highest intra-cluster distance, indicating the presence of heterogeneity among the landraces and they can be parents in hybridization programme.
The genetic variation and relationships among 16 pumpkin accessions of Cucurbita moschata & Cucurbita maxima were assessed based on variation in fruit shape, skin color, flesh and size of fruits. Polyacrylamide gel electrophoresis of seed protein and molecular markers revealed by SCoT and ISSR techniques. The SDS-PAGE electropherogram showed 12 bands; two (75 and 145 kDa) were found in accessions (1-8) and one (85 kDa) was found in accessions 9-16. Other three bands were unique to accessions 1, 2 & 11. Five ISSR primers produced 79 markers ranging in molecular size from 130 to 2140 bp. Six SCoT primers produced 173 markers ranging from 135 to 2660 bp. The ISSR polymorphism among the examined accessions was 100% in the case of primers 49A, 44 B and HB-12, and was 92% for primer HB-15. Similarly, 100% polymorphism was scored for the primers SCoT8, SCoT11, SCoT12 and SCoT14. The lowest polymorphism was 93.94% in case of primer SCoT1. Our data based on protein, RAPD and ISSR data using the SYSTAT version 7.0 program clearly distinguished accessions from each other.
2000
Knowledge of genetic relationships among genotypes is essential for the effective utilisation of germplasm, especially for poorly characterised species. Random amplified polymorphic DNA (RAPD) analysis provides a quick and reliable method for resolving genetic relationships. Although Cucurbita moschata Duch, also known as tropical pumpkin, is one of the most important vegetable crops in Africa, being adapted to a wide range of climatic and soil conditions, it is a scientifically neglected species. The objectives of this study were to (1) analyse the amount of genetic diversity in C. moschata landraces grown in south-central Africa and classify the landraces to assist in selection of parent genotypes for improvement of fruit characteristics. Cluster analysis, based on 39 polymorphic and 105 monomorphic DNA fragments amplified by 16 primers, was used to show relationships among 31 genotypes obtained from Zambia and Malawi. The analysis revealed four clusters, with genotypes from Malawi mainly clustering in three clusters while all genotypes from Zambia and three from Malawi clustered in one cluster. The pair-wise mean genetic distance was 0.32 ± 0.04 for samples from Malawi and 0.26 ± 0.04 for samples from Zambia. The possible application of the resulting classification in breeding of C. moschata is discussed.
This study was carried out to determine the efficacy of inter simple sequence repeats (ISSR) and sequence-related amplified polymorphism (SRAP) techniques for molecular characterization some Cucurbita genotypes including naked (hull-less) seed pumpkin. Plant material consisted of sixteen genotypes belonging to Cucurbita pepo L., and four genotypes belonging to Cucurbita moschata Duchesne, and four genotypes belonging to Cucurbita maxima Duchesne. A larger part of the Cucurbita pepo genotypes (7 Turkish and 4 foreign) were seed pumpkin and some of them were naked seed types used as appetizer and/or for oil production. Morphological characterization was also done according to UPOV criteria. The sixty bands were obtained by using 8 ISSR primers and all these bands were found polymorphic. In the SRAP study, 8 primer combinations were used, a total of 71 bands were scored and all these bands were polymorphic. In the ISSR analyses, the genetic similarity coefficients varied between 0.07 and 0.96, while in SRAP it was between 0.13 – 1.0. The correlation coefficient between ISSR and SRAP genetic similarity data was very high (r = 0.947). The genetic variation as measured by ISSR and SRAP markers revealed high diversity among Cucurbita genotypes (H = 0.30, I = 0.46 and 100 % polym.). The genetic diversity among Turkish naked seed pumpkin genotypes (H = 0.09, I = 0.14 and 26.7 % polym.) was only a little less than that of the foreign accessions (H = 0.12, I = 0.18 and 32.1 % polym.). Some differences were observed between molecular and morphological studies, and it was concluded that only morphological or molecular analyses but a combination of both approaches are often more reliable in genetic variability studies in Cucurbita genus.
Genetic Resources and Crop Evolution, 2015
Cucurbita pepo is a highly diverse, economically important member of the Cucurbitaceae. C. pepo encompasses hundreds of cultivars of pumpkins, squash, and gourds. Although C. pepo has been scrutinized with various types of DNA markers, the relationships among the cultivar-groups of C. pepo subsp. pepo, the more widely grown subspecies, have not heretofore been adequately resolved. We assessed genetic relationships among 68 accessions of Cucurbita pepo, including 48 from C. pepo subsp. pepo, using polymorphisms in 539 high frequency oligonucleotide-targeting active gene (HFO-TAG) fragments, that preferably represent coding regions of the genome. Dissimilarities among accessions were calculated, a dendrogram was constructed, and principal component analyses were conducted.
2021
Background Plant genetic resources constitute the most valuable assets of countries. It is of great importance to determine the genetic variation among these resources and to use the data in breeding studies. Cucurbita maxima species in the cucurbitaceae family have high genetic diversity, but its genetic diversity at the molecular level is inadequately characterized. Methods and Results To determine the genetic diversity among genotypes of Cucurbita maxima species of squash, which is widely grown in Erzincan, 14 different squash genotypes collected were examined based on the morphological parameters and molecular characteristics. SSR (Simple sequence repeat) markers were used to determine genetic diversity at the molecular level. The analysis of morphological characterization within genotypes showed a wide variability in morphological traits of plant, flower, fruit, and leaf. Seven SSR markers yielded a total of 23 polymorphic bands, the number of alleles per marker ranged from 2 t...