Calcium ionophore A23187 and lymphocyte activation (original) (raw)

1976

Abstract

Publisher Summary This chapter discusses the calcium ionophore and lymphocyte activation. Lymphocyte transformation induced by the mitogenic plant lectins, phytohemagglutinin, and concanavalin A, requires extracellular ionized calcium. These lectins induce prompt increases in Ca2+ uptake. These evidence suggest a central role for Ca2+ in lymphocyte activation. To evaluate the role of calcium in the activation of these cells, effects produced by the calcium ionophore were examined. In doing so, six biochemical parameters of activation were studied: (1) calcium uptake, (2) cyclic nucleotide accumulation, (3) membrane protein phosphorylation, (4) phosphatidylinositol turnover, (5) amino acid transport, and (6) DNA synthesis. Human lymphocytes were purified from peripheral venous blood by dextran sedimentation and isopyenic centrifugation. Ionophore concentrations under 1μg/ml were noncytotoxic. No consistent changes in cyclic GMP values were observed at noncytotoxic concentrations. Increases in lymphocyte membrane protein phosphorylation were seen with the ionophore.

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