Engineered Nanostructured Materials for Ofloxacin Delivery (original) (raw)
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Journal of Porous Materials, 2020
Silver nanoparticles are highly efficient antibacterial agents. The applications of silver nanoparticles in the biological environment are always obstructed by oxidation that causes aggregation of nanoparticles which lowers its antibacterial activity. In this research, silver nanoparticles were coated on the surface of mesoporous silica forming mesoporous silver nanoshells (Ag@ MSNs). The prepared nanoparticles were characterized by their zeta potential, particle size, UV absorption spectroscopy, and transmission electron microscopy. Additionally, Fourier transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC), and X-ray diffraction (XRD) were also performed. The antimicrobial activity of the prepared Ag@MSNs was evaluated against gram-positive and gram-negative pathogens. The results showed that Ag@MSNs are monodispersed with an average size distribution 272 ± 61 nm. Ag@MSNs are highly stable with zeta potential value of 43.3 ± 6.5 mV and an absorption peak at 235 nm. Small-angle X-ray diffraction demonstrated a narrow and strong peak at 2ϴ = 1.71 ο indicating that the Ag@MSNs have an ordered porous structure. Surface coating of mesoporous silica nanoparticles with silver shells increased the contact surface area with the bacterial membrane. Thus, Ag@MSNs significantly inhibited the growth of Staphylococcus aureus and E-coli. In conclusion the present study gives new insights into the synthesis of antimicrobial nano-formulation that can offer a variety of uses in industry and biomedical applications.
International Journal of Nanomedicine, 2021
It is a fact that the use of antibiotics is inducing a growing resistance on bacteria. This situation is not only the consequence of a drugs’ misuse, but a direct consequence of a widespread and continuous use. Current studies suggest that this effect could be reversed by using abandoned antibiotics to which bacteria have lost their resistance© , but this is only a temporary solution that in near future would lead to new resistance problems. Fortunately, current nanotechnology offers a new life for old and new antibiotics, which could have significantly different pharmacokinetics when properly delivered; enabling new routes able to bypass acquired resistances.© In this contribution, we will focus on the use of porous silica nanoparticles as functional carriers for the delivery of antibiotics and biocides in combination with additional features like membrane sensitizing and heavy metal-driven metabolic- disrupting therapies as two of the most interesting combination therapies.
International Journal of Pharmaceutics, 2014
In this paper, we report the synthesis, characterization (FT-IR, XRD, BET, HR-TEM) and bioevaluation of a novel ␥-aminobutiric acid/silica (noted GABA-SiO 2 or ␥-SiO 2 ) hybrid nanostructure, for the improved release of topical antibiotics, used in the treatment of Staphylococcus aureus infections. GABA-SiO 2 showed IR bands which were assigned to Si-O-Si (stretch mode). The XRD pattern showed a broad peak in the range of 18-30 • (2Â), indicating an amorphous structure. Based on the BET analysis, estimations about surface area (438.14 m 2 /g) and pore diameters (4.76 nm) were done. TEM observation reveals that the prepared structure presented homogeneity and an average size of particles not exceeding 10 nm. The prepared nanostructure has significantly improved the anti-staphylococcal activity of bacitracin and kanamycin sulfate, as demonstrated by the drastic decrease of the minimal inhibitory concentration of the respective antibiotics loaded in the GABA-SiO 2 nanostructure. These results, correlated with the high biocompatibility of this porous structure, are highlighting the possibility of using this carrier for the local delivery of the antimicrobial substances in lower active doses, thus reducing their cytotoxicity and side-effects.
Silica-Antibiotic Hybrid Nanoparticles for Targeting Intracellular Pathogens
Antimicrobial Agents and Chemotherapy, 2009
We investigated the capability of biodegradable silica xerogel as a novel carrier of antibiotic and the efficacy of treatment compared to that with the same dose of free drug against murine salmonellosis. The drug molecules (31%) entrapped in the sol-gel matrix remained in biologically active form, and the bactericidal effect was retained upon drug release. The in vitro drug release profiles of the gentamicin from the xerogel and that from the xerogel-polyethylene glycol (PEG) were distinctly different at pH 7.4. A delayed release of gentamicin was observed from the silica xerogel network (57% in 33 h), and with the addition of 2% PEG, the release rate reached 90% in 33 h. Administration of two doses of the silica xerogel significantly reduced the Salmonella enterica serovar Typhimurium load in the spleens and livers of infected AJ 646 mice. The silica xerogel and xerogel-PEG achieved a 0.45-log and a 0.41-log reduction in the spleens, respectively, while for the free drug there was no reduction. On the other hand, silica xerogel and xerogel-PEG achieved statistically significant 1.13-log and 1.15-log reductions in the livers, respectively, while for the free drug the reduction was a nonsignificant value of 0.07 log. This new approach, which utilizes a room-temperature synthetic route for incorporating therapeutic drugs into the silica matrix, should improve the capability for targeting intracellular pathogens.
Pharmaceutics, 2021
This review focuses on the design of mesoporous silica nanoparticles for infection treatment. Written within a general context of contributions in the field, this manuscript highlights the major scientific achievements accomplished by professor Vallet-Regí’s research group in the field of silica-based mesoporous materials for drug delivery. The aim is to bring out her pivotal role on the envisage of a new era of nanoantibiotics by using a deep knowledge on mesoporous materials as drug delivery systems and by applying cutting-edge technologies to design and engineer advanced nanoweapons to fight infection. This review has been divided in two main sections: the first part overviews the influence of the textural and chemical properties of silica-based mesoporous materials on the loading and release of antibiotic molecules, depending on the host–guest interactions. Furthermore, this section also remarks on the potential of molecular modelling in the design and comprehension of the perfo...
ACS Applied Biomaterials, 2020
Pseudomonas aeruginosa (PA) is an opportunistic pathogen, which causes serious lung infections in immunocompromised patients. Traditional oral intake of large quantities of small-molecule antibiotics to treat bacterial infections leads to off-target toxicity and development of drug-resistant species. Improved delivery systems of antibiotics to the targeted site of bacterial infections would help reduce the need for a high intake of antibiotics. Colistin (Col), an antibacterial peptide, is considered the last resort treatment for multidrug resistant (MDR)-PA. To approach the problem of development of antibacterial resistance and off-target toxicity due to the use of excessive amounts of antibiotics, we have designed a targeted drug delivery nanoassembly, which delivers antibiotics to extracellular and intracellular bacteria. The nanoassembly is composed of (1) drug (Col)-loaded mesoporous silica (MSN) core (Col@MSN), (2) liposomal shell (Col@ MSN@LL), and (3) PA-targeting LL-37 peptide (Col@MSN@LL-(LL-37)). The liposomal shell prevents premature drug release before the nanoassembly approaches the targeted bacteria. The liposome bilayer degrades upon excreted lipase present in the local environment of PA, releasing encapsulated Col. There is a significant increase in Col release (∼90% release within 40 h) in the presence of bacteria compared to the absence of bacteria (only ∼75% release after 80 h). A 6.7-fold increase in the antimicrobial efficacy of Col encapsulated in Col@MSN@LL-(LL-37) was seen compared to free Col. All studies were done using a clinical strain of PA14. Col@MSN@LL-(LL-37) successfully targets and inhibits intracellular PA14 within the lung epithelial cells. Only 7% PA14 viability is seen after treating the lung epithelial cells with Col@MSN@LL-(LL-37). No significant cytotoxicity was observed with Col@MSN@LL-(LL-37). Therefore, this discussed lipid-coated targeted nanoassembly can be considered as a successful antibiotic delivery platform.
International Journal of Pharmaceutics, 2016
This work reports the non-surfactant templated synthesis and characterization of a new tyrosine-silica/ antibiotics (TyR-SiO 2 /ATBs) nanocomposite, as well as both in vitro and in vivo cytotoxicity and antimicrobial activity against the microbial pathogen Staphylococcus aureus. The in vitro microbiological tests proved that the obtained nanobiostructure significantly enhance the antimicrobial activity of three commonly used antibiotics against S. aureus (i.e. erythromycin (ERI), gentamicin (GEN), and cloxacillin (CLO)) as revealed by the increased diameters of the growth inhibition zones and the decreased minimal inhibitory concentration values, as well as by the inhibitory effect of sub-lethal antibiotic concentrations on the ability of the respective pathogenic strains to adhere and colonize different substrata. These results, correlated with the lack of toxicity against mesenchymal stem cells along with an appropriate in vivo biodistribution highlight the promising therapeutic potential of this carrier that allows a decrease of the required active doses while significantly lessening the harmful side effects of the medication on the host organism. 2016 Elsevier B.V. All rights reserved.
Pharmaceutics, 2021
Mesoporous silica nanoparticles (MSNs) are very promising nanomaterials for treating bacterial infections when combined with pharmaceutical drugs. Herein, we report the preparation of two nanomaterials based on the immobilization of ciprofloxacin in mesoporous silica nanoparticles, either as the counter-ion of the choline derivative cation (MSN-[Ch][Cip]) or via anchoring on the surface of amino-group modified MSNs via an amide bond (MSN-Cip). Both nanomaterials were characterized by TEM, FTIR and solution 1H NMR spectroscopies, elemental analysis, XRD and N2 adsorption at 77 K in order to provide the desired structures. No cytotoxicity from the prepared mesoporous nanoparticles on 3T3 murine fibroblasts was observed. The antimicrobial activity of the nanomaterials was determined against Gram-positive (Staphylococcus aureus and Bacillus subtilis) and Gram-negative (Klebsiella pneumoniae) bacteria and the results were promising against S. aureus. In the case of B. subtilis, both nano...
Microporous and Mesoporous Materials, 2012
Mesoporous silica (SBA-15) with peracetic acid (PAA) adsorbed on its mesopores together with silver nanoparticles attached to their pore walls show a strong bactericidal effect on antibiotic-resistant and biofilm forming Staphylococcus aureus, considerably larger than that expected from the summation of the independent effects of both antimicrobials. Ag nanoparticles were formed in situ, by loading silver nitrate in the pores of the amino-functionalized mesoporous silica, followed by the addition of a reducing agent. The size of the silver nanoparticles could be tuned depending on the amount of silver precursor. The ordered mesoporous structure of SBA-15 is preserved after the formation of Ag nanoparticles and subsequent PAA loading which allows for the sustained release of both agents. Therefore, in this work, a longterm antimicrobial releasing nanostructured carrier with a synergistic bactericidal action is presented.