Bioethanol production from sugarcane molasses by instant dry yeast (original) (raw)
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IOP Conference Series: Earth and Environmental Science
Some bioethanol industries in Indonesia that use sugarcane molasses as raw material often face low level of ethanol content from fermentation process. The molasses condition and the temperature fluctuation are supposed to be the cause. This research aimed to determine the effect of sugarcane molasses pre-treatment with H 2 SO 4 and fermentation temperature in bioethanol production by using instant dry yeast of Saccharomyces cerevisiae. Factorial Randomized Block Design with 2 factors was used in the research. The first factor was pre-treatment effect in sugarcane molasses (with and without pre-treatment) and the second was fermentation temperature (29 0 C, 32 0 C and 35 0 C). The data were analysed using analysis of variance or ANOVA and continued with further test Duncan Multiple Range Test or HSD (Honestly Significant Difference) with confidence interval of 5%. The best treatment was determined using Multiple Attribute Test. Results showed that sugarcane molasses pretreatment with H 2 SO 4 and fermentation temperature had a significant effect (α= 0.05) on bioethanol production. The best result was obtained from sugarcane molasses medium that given H 2 SO 4 pre-treatment and fermentation temperature of 32 0 C with total decrease value of total soluble solids (% brix) of 10.9 % brix, total decrease sugar of 12.15%, reducing sugar consumption of 57.21 g/L, ethanol content of 8.30 % and yield ethanol of 68.67 %.
2007
The present study analyzes the influence of modification of sugar crystallization from three to two stages and tried to optimize both sugar and ethanol production from sugar cane source. The efficiency of sugar crystallization process was determined by simulation at Wonji Shoa Sugar Factory (WSSF). Economical advantages in optimizing the sugar and ethanol productions were compared with the factory's existing working norms. Batch fermentation of molasses samples using saccharomyces cerevisiae was carried out at fermentation temperature of 30 (+1) o C, pH of 5.5 (+ 0.1) and ammonia supplement of 2 g/L in Erlenmeyer flask of 250 mL. The parameters varied were total sugar as invert (TSAI) in the fermentation broth of prepared molasses from both two and three crystallization stages and the amount of dry yeast in the inoculums (1,3,5 g/L). The measured values were concentration of the produced alcohol and the residual sugar. From the obtained data of alcohol concentration and residual...
Microbiology and Biotechnology Letters
Bioethanol has recently attracted much attention as a sustainable and environmentally friendly alternative energy source. This study aimed to develop a potential process for bioethanol production by fed-batch fermentation using instant dry yeast. To obtain the highest cell growth, we studied the influence of the initial sugar concentrations and pH of sugarcane molasses in batch fermentation. The batch system employed three levels of sugar concentrations, viz. 10%, 15%, 20% (w/v), and two levels of pH, 5.0 and 5.5. The highest cell growth was achieved at 20% (w/v) and pH 5.5 of molasses. The fed-batch system was then performed using the best batch fermentation conditions, with a molasses concentration of 13% (w/v) which resulted in high ethanol concentration and fermentation efficiency of 15.96% and 89%, respectively.
Bioprocess and Biosystems Engineering, 2011
Molasses ''B'' is a rich co-product of the sugarcane process. It is obtained from the second step of crystallization and is richer in fermentable sugars (50-65%) than the final molasses, with a lower non-sugar solid content (18-33%); this co-product also contains good vitamin and mineral levels. The use of molasses ''B'' for ethanol production could be a good option for the sugarcane industry when cane sugar prices diminish in the market. In a complex medium like molasses, osmotolerance is a desirable characteristic for ethanol producing strains. The aim of this work was to evaluate the use of molasses ''B'' for ethanol production using Saccharomyces cerevisiae ITV-01 (a wild-type yeast isolated from sugarcane molasses) using different initial sugar concentrations (70-291 g L-1), two inoculum sizes and the addition of nutrients such as yeast extract, urea, and ammonium sulphate to the culture medium. The results obtained showed that the strain was able to grow at 291 g L-1 total sugars in molasses ''B'' medium; the addition of nutrients to the culture medium did not produce a statistically significant difference. This yeast exhibits high osmotolerance in this medium, producing high ethanol yields (0.41 g g-1). The best conditions for ethanol production were 220 g L-1 initial total sugars in molasses ''B'' medium, pH 5.5, using an inoculum size of 6 9 10 6 cell mL-1 ; ethanol production was 85 g L-1 , productivity 3.8 g L-1 h-1 with 90% preserved cell viability.
Production of Ethanol From Sugar Cane Molasses and Evaluation of Its Quality
The objective of the present study was to produce ethanol from final sugar cane molasses and to evaluate its quality. Final cane molasses samples were obtained from Elguneid Sugar Factory. Urea was added in different concentrations (0.15%, 0.5%, and 0.25%) and used as nitrogen source to determine optimum concentration. Experiments were conducted using four treatments depending upon molasses sugar concentration which was calculated as percentages (10, 15, 20 and 25). The pH of the mash was adjusted to 4.8 using conc. sulphuric acid. 5% baker's yeast (DCL) was added. The fermentation was conducted for 72 hours, at 33ْ C. The microbiological analysis revealed absence of bacteria, yeasts and moulds in dilutions 10 -3 , 10 -4 , 10 -5 of molasses samples. The yield ethanol obtained was 20 ml per 100 gm of molasses, and the maximum concentration of ethanol (96%) could be obtained when the main medium of production) molasses) includes 0.25% urea and 20% sugar concentration.
2012
A repeated batch fermentation system was used to produce ethanol using Saccharomyces cerevisiae strain (NCIM 3640) immobilized on sugarcane (Saccharum officinarum L.) pieces. For comparison free cells were also used to produce ethanol by repeated batch fermentation. Scanning electron microscopy evidently showed that cell immobilization resulted in firm adsorption of the yeast cells within subsurface cavities, capillary flow through the vessels of the vascular bundle structure, and attachment of the yeast to the surface of the sugarcane pieces. Repeated batch fermentations using sugarcane supported biocatalyst were successfully carried out for at least ten times without any significant loss in ethanol production from sug-arcane juice and molasses. The number of cells attached to the support increased during the fermentation process, and fewer yeast cells leaked into fermentation broth. Ethanol concentrations (about 72.65~76.28 g/L in an average value) and ethanol productivities (abou...
Mycobiology, 2012
A repeated batch fermentation system was used to produce ethanol using Saccharomyces cerevisiae strain (NCIM 3640) immobilized on sugarcane (Saccharum officinarum L.) pieces. For comparison free cells were also used to produce ethanol by repeated batch fermentation. Scanning electron microscopy evidently showed that cell immobilization resulted in firm adsorption of the yeast cells within subsurface cavities, capillary flow through the vessels of the vascular bundle structure, and attachment of the yeast to the surface of the sugarcane pieces. Repeated batch fermentations using sugarcane supported biocatalyst were successfully carried out for at least ten times without any significant loss in ethanol production from sugarcane juice and molasses. The number of cells attached to the support increased during the fermentation process, and fewer yeast cells leaked into fermentation broth. Ethanol concentrations (about 72.65~76.28 g/L in an average value) and ethanol productivities (about 2.27~2.36 g/L/hr in an average value) were high and stable, and residual sugar concentrations were low in all fermentations (0.9~3.25 g/L) with conversions ranging from 98.03~99.43%, showing efficiency 91.57~95.43 and operational stability of biocatalyst for ethanol fermentation. The results of the work pertaining to the use of sugarcane as immobilized yeast support could be promising for industrial fermentations.
Abstract: India is the second largest producer of sugarcane in the world. Hence the sugarcane juice can be a good raw material for the production of ethanol in our country. Ethanol is primarily used for potable purpose, for the production of various chemicals and in blending with petrol and diesel. Lab scale fermentation of sugarcane juice was carried out using yeast species saccharomyces cerevecia. The fermentation was carried out in different batches with varying sugar concentration. The obtained results are tabulated. Inversion of sugarcane juice was done prior to the fermentation and the results obtained were compared with the results obtained from fermentation non pre-inverted sugarcane juice. This showed a marked difference in the fermentation time for the same conversion. The comparison of process of production of ethanol from sugarcane juice and molasses s also made and the probable process modification for shift of molasses based distilleries to juice based distilleries is proposed. Keywords: Saccharomyces cerevecia, Inversion, Fermentation
Impact of Storage Time, Rain and Quality of Molasses in the Production of Bioethanol
Due to environmental concerns and the rising prices of petroleum products, usually bio-ethanol is being produced in greater amount from sugar cane molasses in Pakistan. In this work various properties related to molasses are being analyzed by performing experiments at the Distillery Plant, AASML (Al-Abbas Sugar Mills Limited), Mirpurkhas, Sindh, Pakistan. Brix, Sucrose, pH, ethyl alcohol, RS (Reducing Sugar), TSI (Total Sugar as an Invert), TS (Total Sugar), purity and presence of micro-organisms are